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An epidemic methicillin-resistant strain of Staphylococcus aureus in Spain

  • P. Aparicio (a1) (a2), J. Richardson (a2), S. Martin (a2), A. Vindel (a1), R. R. Marples (a2) and B. D. Cookson (a2)...

Summary

During 1990, a strain of methicillin-resistant Staphylococcus aureus became epidemic in Spain and spread in a manner analogous to that of EMRSA-1 in England. Isolates of this strain produced little protein A and were resistant to a number of antibiotics including ciprofloxacin. Beta-lactamase production was encoded by a c. 39 kb plasmid, which also conferred resistance to mercury, cadmium, ethidium bromide and propamidine isethionate.

Investigation showed that two variants, separable by supplementary and Fisk phage typing, were circulating. The B variant appeared to spread more readily than the A variant.

The opportunity was taken to compare the discriminatory power of traditional typing methods with molecular techniques. The discriminatory power of the molecular techniques used only reached the same level as the traditional methods when double enzyme digestion of total cellular DNA by EcoR I and Cla I was performed.

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Copyright

Corresponding author

*Dr J. F. Richardson, Staphylococcus Reference Laboratory. Central Public Health Laboratory. 61 Colindale Avenue. London NW9 5HT.

References

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1.Williams, REO. Epidemic staphylococci. Lancet 1959; i: 190–5.
2.Townsend, DE, Ashdown, N, Bolton, S et al. , The international spread of methicillin-resistant Staphylococcus aureus. J Hosp Infect 1987; 9: 6071.
3.Marples, RR, Cooke, EM. Current problems with methicillin-resistant Staphylococcus aureus. J Hosp Infect 1988; 11: 381–92.
4.Kerr, S, Kerr, GE, Mackintosh, CA, Marples, RR. A survey of methicillin-resistant Staphylococcus aureus in England and Wales. J Hosp Infect 1990; 16: 3548.
5.Marples, RR. Methicillin-resistant Staphylococcus aureus Curr Opin Inf Dis 1988; 1: 722–6.
6.Bouza, E, Martinez-Beltran, J. Estudio multicentro sobre la prevalencia de estafilocoeos en Espana (informe preliminar). Enfermedardes Infecciosas y Microbiologia Clinica 1988; 6: 6879.
7.Phillips, I, Andrews, JM, Burt, AJ et al. , A guide to sensitivity testing. J Antimicrob Chemother 1991; 27 Suppl D: 29.
8.Burnet, FM. The production of staphylococcal toxin. J Pathol Bacteriol 1930; 33: 116.
9.Novick, R. Properties of a cryptic high-frequency transducing phage in Staphylococcus aureus. Virology 1967; 33: 155166.
10.Jevons, MP.‘Celbenin’-resistant staphylococci. B M J 1961; 1: 124–5.
11.Kloos, WE, Schleifer, KH. Isolation and characterization of staphylococci from the human skin. II Description of four new species: Staphylococcus warneri. Staphylococcus capitis. Staphylococcus hominis and Staphylococcus simulans. Int J Syst Bacteriol 1975; 25: 6279.
12.Marples, RR, Richardson, JF, de Saxe, MJ. Bacteriological characters of strains of Staphylococcus aureus submitted to a reference laboratory related to methicillin resistance. J Hyg 1986; 96: 217–23.
13.Richardson, JF, Marples, RR. Another strain of methicillin-resistant Staphylococcus aureus epidemic in London. Lancet 1988; ii: 748–9.
14.Winkler, KCde Waarf, J, Grootsen, C, Zegers, BJM, Tellier, NF, Wertregt, CD. Lysogenic conversion of staphylococci to loss of β toxin. J Gen Microbiol 1965; 39: 321–33.
15.Blair, JE, Williams, REO. Phage typing of staphylococci. Bull World Hlth Org 1961; 24: 771–84.
16.Subcommittee on the phage typing of staphylococci. Int J Syst Bact 1975; 25: 6774.
17.Richardson, JF, Chittasobhon, N, Marples, RR. A supplementary phage set for the investigation of methicillin-resistant strains of Staphylococcus aureus J Med Microbiol 1988; 25: 6774.
18.Fisk, RT. Studies on staphylococci II. Identification of Staphylococcus aureus strains by means of bacteriophage. J Infect Dis 1942; 71: 161–5.
19.De Saxe, MJ, Notley, CM. Experiences with the typing of coagulase-negative staphylococci and micrococci. Zbl Bakt Hyg I Abt. Orig A 1978; 241: 4659.
20.Vickery, AM, Beard-Pegler, MA, Rountree, PM. Strain differentiation in methicillin-resistant Staphylococcus aureus. Pathology 1983; 15: 235–40.
21.Rippon, JE. The classification of bacteriophages lysing staphylococci. J Hyg 1956; 54. 213–26.
22.Simkovicova, M, Gilbert, RJ. Serological detection of enterotoxin from food poisoning strains of Staphylococcus aureus. J Med Microbiol 1971; 4: 1930.
23.Cowan, ST. Cowan and Steel's manual for the identification of medical bacteria. 2nd edn.Cambridge: Cambridge University Press, 1974.
24.Richardson, JF, Quoraishi, AHM, Francis, BJ, Marples, RR.Beta-lactamase negative methicillin-resistant Staphylococcus aureus in a newborn nursery: report of an outbreak and laboratory investigations. J Hosp Infect 1990; 16: 109–21.
25.Ericsson, H. Standardisation of methods for conducting sensitivity tests. Stockholm, Sweden: Karolinska Sjkhuset, 1964.
26.Richardson, JF, Marples, RR. Differences in antibiotic susceptibility between Staphylococcus epidermidis and Staphylococcus saprophyticus. J Antimicrob Chemother 1980; 6: 499510.
27.Richardson, JF, Marples, RR, Kerr, GE. Epidemiological typing of Staphylococcus aureus. Zbk Bakt Suppl 1991; 21: 389–93.
28.Maniatis, T, Fitsch, EF, Sambrook, J.Molecular cloning: a laboratory manual. Cold Spring Harbor: Cold Spring Harbor Press, 1982.
29.Pitcher, DG, Owen, RJ, Dyal, P, Beck, A. Synthesis of a biotinylated DNA probe to detect ribosomal DNA cistrons in Providencia stuartii. FEMS Microbiol Lett 1987; 48: 283–7.
30.Richardson, JF, Noble, WC, Marples, RR. Identification and epidemiological typing of the staphylococci. In: Identification methods in applied and environmental microbiology. Skinner, FA, Jones, D, eds. Society for Applied Bacteriology. Technical Series 29. In press.

An epidemic methicillin-resistant strain of Staphylococcus aureus in Spain

  • P. Aparicio (a1) (a2), J. Richardson (a2), S. Martin (a2), A. Vindel (a1), R. R. Marples (a2) and B. D. Cookson (a2)...

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