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Age-dependent prevalence of BK virus IgG and IgM antibodies measured by enzyme-linked immunosorbent assays (ELISA)

Published online by Cambridge University Press:  19 October 2009

Trond Flaegstad ,
Terje Traavik  and
Bjørn-Erik Kristiansen
Trond Flaegstad
Affiliation:
Departments of Microbiology, (Institute of Medical Biology), and Paediatrics (Institute of Clinical Medicine)
Terje Traavik
Affiliation:
Department of Microbiology, (Institute of Medical Biology) University of Tromsø, N–9012 Tromsø, Norway
Bjørn-Erik Kristiansen
Affiliation:
Department of Microbiology, (Institute of Medical Biology) University of Tromsø, N–9012 Tromsø, Norway
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Summary

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Enzyme immunoassays (ELISA) have been developed for the detection of BK virus IgG- and IgM-antibodies. Specific IgG is detected by an antigen-coated solid phase test; IgM by an antibody capture method.

These methods have been used to study the age-distribution of BK virus antibodies in Troms county in Northern Norway. The serum panels tested were: (a) 60 sera from paediatric patients aged 0–1 year; (b) 220 sera from healthy persons aged 1–82 years; (c) 74 sera from healthy blood donors; (d) 107 sera from healthy pregnant women.

The age-distribution of BKV-IgG antibodies showed that primary infections took place predominantly between the ages of 1 and 6 years, and that there were no sex differences, either in the age-specific prevalence or in the level of BKV-IgG.

We found no significant differences in the prevalence of BKV-IgM antibodies in healthy children and adults and pregnant women. BKV-IgM was detected in 26 of the 461 sera tested (5·6%).

Type
Research Article
Information
Journal of Hygiene , Volume 96 , Issue 3 , June 1986 , pp. 523 - 528
Copyright
Copyright © Cambridge University Press 1986

References

REFERENCES

Brown, D. W. G., Gardner, S. D., Gibson, P. E. & Field, A. M. (1984). BK virus specific IgM responses in cord sera, young children and healthy adults detected by RIA. Archives of Virology 82, 149–160.CrossRefGoogle Scholar
Brown, P., Tsai, T. & Gajdusek, D. C. (1975). Seroepidemiology of human papovaviruses. Discovery of virgin populations and some unusual patterns of antibody prevalence among remote peoples of the world. American Journal of Epidemiology 102, 331–340.CrossRefGoogle ScholarPubMed
Dei, R., Marmo, F., Corte, D., Sampietro, M. G., Franceschini, E. & Urbano, P. (1982). Age-related changes in the prevalence of precipitating antibodies to BK virus in infants and children. Journal of Medical Microbiology 15, 285–291.CrossRefGoogle Scholar
Flower, A. J. E., Banatvala, J. E. & Chrystie, I. L. (1977). BK antibody and virus specific IgM responses in renal transplant recipients, patients with malignant disease, and healthy people. British Medical Journal 2, 220–223.CrossRefGoogle ScholarPubMed
Flaegstad, T. & Traavik, T. (1985 a). Detection of BK virus antibodies measured by ELISA assay and two haemagglutination inhibition methods. A comparative study. Journal of Medical Virology 16, 351–356.CrossRefGoogle ScholarPubMed
Flaegstad, T. & Traavik, T. (1985 b). Detection of BK virus IgM antibodies by two enzyme-linked immunosorbent assays (ELISA) and a hemagglutination inhibition method. Journal of Medical Virology 17, 195–204.CrossRefGoogle Scholar
Gardner, S. D., Field, A. M., Coleman, D. V. & Hulme, B. (1971). New human papovavirus (B.K.) isolated from urine after renal transplantation. Lancel i, 1253–1257.CrossRefGoogle Scholar
Gardner, S. D. (1973). Prevalence in England of antibody to human polyomavirus (B.K.). British Medical Journal 1, 77–78.CrossRefGoogle ScholarPubMed
Gibson, P. E., Field, A. M., Gardner, S. D. & Coleman, D. V. (1981. Occurrence of IgM antibodies against BK and JC polyomaviruses during pregnancy. Journal of Clinical Pathology 34, 674–679.CrossRefGoogle ScholarPubMed
Goudsmit, J., Wertheim-van Dillen, P., van Strien, A. & van der Noordaa, J. (1982). The role of BK virus in acute respiratory tract disease and the presence of BKV DNA in tonsils. Journal of Medical Virology 10, 91–99.CrossRefGoogle ScholarPubMed
Jung, M., Krech, U., Price, P. C. & Pyndiah, M. N. (1975). Evidence of chronic persistent infections with polyomavirus (BK type) in renal transplant recipients. Archives of Virology 47, 39–46.CrossRefGoogle ScholarPubMed
Mäntyjärvi, R. A. (1979). New oncogenic human papova-viruses. Medical Biology 57, 29–35.Google Scholar
Rziha, H. J., Bornkamm, G. W. & zur Hausen, H. (1978). BK virus: I. Sero-epidemiologic studies and serologic response to viral infection. Medical Microbiology and Immunology 165, 73–81.CrossRefGoogle Scholar
Rziha, H. J., Belohradsky, B. H., Schneider, U., Schwenk, H. U., Bornkamm, G. W. & zur Hausen, H. (1978). BK virus. II. Serologic studies in children with congenital disease and patients with malignant tumors and immunodeficiencies. Medical Microbiology and Immunology 165, 83–92.CrossRefGoogle ScholarPubMed
Shah, K. V., Daniel, R. W. & Warszawski, R. M. (1973). High prevalence of antibodies to BK virus, an SV40-related papovavirus, in residents of Maryland. Journal of Infectious Diseases 128, 784–787.CrossRefGoogle Scholar
Shah, K.Daniel, R., Madden, D. & Stagno, S. (1980). Serological investigation of BK papovavirus infection in pregnant women and their offspring. Infection and Immunity 30, 29–35.CrossRefGoogle ScholarPubMed