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Lack of stable inheritance of introgressed transgenefrom oilseed rape in wild radish

Published online by Cambridge University Press:  15 June 2005

Anoir Al Mouemar  and
Henri Darmency
Anoir Al Mouemar
Affiliation:
Department of Plant Protection, Faculty of Agronomy, Damas University, Syria
Henri Darmency
Affiliation:
Unité Mixte de Recherche sur la Biologie et la Gestion des Adventices, INRA, BP 86510, 21065 Dijon, France

Abstract

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Hybridization of Brassica napus L. (oilseed rape) and Raphanus raphanistrum L. (wild radish) has been demonstrated, and may be the first step towards introgression of transgenes in this wild relative. If wild radish were to display a new adaptive advantage by expressing the transgene, this could modify the ecological balance of species within the agro-ecosystem. To determine if transgenes remained stable in the hybrid, the frequency of herbicide resistance was studied over four advanced generations of hybrid progeny (G8 to G11) that were subjected to herbicide selection pressure. It is expected that hemizygous resistant plants containing an herbicide resistance transgene back-crossed to wild radish would have 50% resistant progeny. In each of the G8 to G11 generations, only 18% of the progeny from resistant plants were resistant. The chromosome complement of herbicide-susceptible progenies, analyzed at G9, was not different from that of wild populations of wild radish. Herbicide-resistant G9 progeny showed higher chromosome instability, and one third of the progeny contained a supernumerary chromosome. These results suggest that in the presence of herbicide selection pressure, the transgene for herbicide resistance would be maintained despite a lack of stabilized introgression. In the absence of selection, the frequency of resistance in the population is expected to decline.

Keywords

transgeneherbicide resistanceinheritancehybridizationintrogressionchromosomeBrassica napusRaphanus raphanistrum
Type
Research Article
Information
Environmental Biosafety Research , Volume 3 , Issue 4 , October 2004 , pp. 209 - 214
Copyright
© ISBR, EDP Sciences, 2004

References

Baranger, A, Chèvre, AM, Eber, F, Renard, M (1995) Effect of oilseed rape genotype on the spontaneous hybridization rate with a weedy species: an assessment of transgene dispersal. Theor. Appl. Genet. 91: 956–963
Baranger, A, Delourme, R, Foisset, N, Barret, P, Dupuy, P, Renard, M, Chèvre, AM (1997) Wide mapping of a T-DNA insertion site in oilseed rape using Bulk Segregant Analysis and comparative mapping. Plant Breed. 116: 553–560 CrossRef
Benabdelmouna A, Guéritaine G, Abirached-Darmency M, Darmency H (2003) Genome discrimination in progeny of interspecific hybrids between Brassica napus and Raphanus raphanistrum. Genome 46: 469–472 CrossRef
Chèvre, AM, Eber, F, Baranger, A, Renard, M (1997a) Gene flow from transgenic crops. Nature 389: 924 CrossRef
Chèvre AM, Eber F, Renard M, Darmency H (1999) Gene flow from oilseed rape to weeds. In Gene flow and agriculture: relevance for transgenic crops, B.C.P.C. Symp. Proc. N° 72, Lutman P Ed., Farnham, UK, pp 125–130
Chèvre, AM, Eber, F, Barret, P, Dupuy, P, Brace, J (1997b) Identification of the different Brassica nigra chromosomes from both sets of B. oleracea-B. nigra and B. napus-B. nigra addition lines with a special emphasis on chromosome transmission and self-incompatibility. Theor. Appl. Genet. 94: 603–611
Chèvre, AM, Eber, F, Baranger, A, Hureau, G, Barret, P, Picault, H, Renard, M (1998) Characterization of backcross generations obtained under field conditions from oilseed rape-wild radish F1 interspecific hybrids: an assessment of transgene dispersal. Theor. Appl. Genet. 97: 90–98
Chèvre, AM, Eber, F, Darmency, H, Fleury, A, Picault, H, Letanneur, JC, Renard, M (2000) Assessment of interspecific hybridization between transgenic oilseed rape and wild radish under agronomic conditions. Theor. Appl. Genet. 100: 1233–1239
Darmency, H, Lefol, E, Fleury, A (1998) Spontaneous hybridizations between oilseed rape and wild radish. Mol. Ecol. 7: 1467–1473 CrossRef
De Block, M, De Brouwer, D, Tennig, P (1989) Transformation of Brassica napus and Brassica oleracea using Agrobacterium tumefaciens and the expression of the bar and neo genes in the transgenic plants. Plant Physiol. 91: 694–701 CrossRef
Eber F, Chèvre AM, Baranger A., Vallée P, Tanguy X, Renard M (1994) Spontaneous hybridization between a male-sterile oilseed rape and two weeds. Theor. Appl. Genet. 88: 362–368
Ellstrand, NC, Prentice, HC, Hancock, JF (1999) Gene flow and introgression from domesticated plants into their wild relatives. Annu. Rev. Ecol. Syst. 30: 539–563 CrossRef
Giancola, S, Marhadour, S, Desloire, S, Clouet, V, Falentin-Guyomarc’h H, Laloui, W, Falentin, C, Pelletier, G, Renard, M, Bendahmane, A, Delourme, R, Budar, F (2003) Characterization of a radish introgression carrying the Ogura fertility restorer gene Rfo in rapeseed, using the Arabidopsis genome sequence and radish genetic mapping. Theor. Appl. Genet. 107: 1442–1451 CrossRef
Guéritaine G, Bazot S, Darmency H (2003) Emergence and growth of hybrids between Brassica napus and Raphanus raphanistrum. New Phytol. 158: 561–567
Guéritaine, G, Sester, M, Eber, F, Chèvre, AM, Darmency, H (2002) Fitness of backcross six of hybrids between transgenic oilseed rape (Brassica napus) and wild radish (Raphanus raphanistrum). Mol. Ecol. 11: 1419–1426 CrossRef
Rieger, MA, Potter, TD, Preston, C, Powles, SB (2001) Hybridization between Brassica napus L. and Raphanus raphanistrum L. under agronomic field conditions. Theor. Appl. Genet. 103: 555–560 CrossRef
Stewart CN, Halfhill MD, Warwick SI (2003) Transgene introgression from genetically modified crops to their wild relatives. Nature Rev. Genet. 4: 806–817
SYSTAT (2000) version 10, SPSS Inc., Chicago
Voss, A, Snowdon, RJ, Lühs, W, Friedt, W (2000) Intergeneric transfer of nematode resistance from Raphanus sativus into the Brassica napus genome. Acta Hort. 539: 129–134 CrossRef
Warwick, SI, Simard, MJ, Légère, A, Beckie, HJ, Braun, L, Zhu, B, Mason, P, Séguin-Swartz, G, Stewart, CN (2003) Hybridization between transgenic Brassica napus L. and its wild relatives: Brassica rapa L., Raphanus raphanistrum L., Sinapis arvensis L., and Erucastrum gallicum (Willd.) O.E. Schulz. Theor. Appl. Genet. 107: 528–539 CrossRef