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Field evidence for the exposure of ground beetles to Cry1Ab from transgenic corn

Published online by Cambridge University Press:  15 November 2005

Claudia Zwahlen  and
David A. Andow
Claudia Zwahlen
Affiliation:
Department of Entomology, University of Minnesota, 219 Hodson Hall, St. Paul, MN 55108, USA
David A. Andow
Affiliation:
Department of Entomology, University of Minnesota, 219 Hodson Hall, St. Paul, MN 55108, USA

Abstract

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Non-target organisms associated with the soil might be adversely affected by exposure to the Cry1Ab protein from Bacillus thuringiensis (Bt) in transgenic corn (Zea mays L.). To check for such exposure, we used ELISA to test for Cry1Ab in ground beetles collected live from fields with Bt corn residues and Bt corn (Bt/Bt), Bt corn residues and non-Bt crops (Bt/non-Bt), or non-Bt corn residues and non-Bt crops (non-Bt/non-Bt). In fields with Bt corn residues (Bt/Bt and Bt/non-Bt), Cry1Ab was present in all seven species of ground beetles examined (Agonum placidum, Bembidion rupicola, Clivina impressefrons, Cyclotrachelus iowensis, Harpalus pensylvanicus, Poecilus chalcites, and Poecilus lucublandus). For the two most abundant species, P. chalcites and P. lucublandus, the proportion of beetles with Cry1Ab was significantly higher in Bt/Bt fields (0.50–1.0) and Bt/non-Bt fields (0.41–0.50) than in non-Bt/non-Bt fields (0.0). This is the first field evidence that some ground beetle species are exposed to Cry1Ab. The implications of exposure on the performance of these non-target organisms are unclear.

Keywords

genetically modified Bt cropsBacillus thuringiensisCarabidaerisk assessmentELISA (enzyme-linked immunosorbent assay)
Type
Research Article
Information
Environmental Biosafety Research , Volume 4 , Issue 2 , April 2005 , pp. 113 - 117
Copyright
© ISBR, EDP Sciences, 2005

References

Ahmad, A, Wilde, GE, Zhu, KY (2005) Detectability of Coleopteran-specific Cry3Bb1 protein in soil and its effect on nontarget surface and below-ground arthropods. Environ. Entomol. 34: 385–394 CrossRef
Clark, MS, Luna, JM, Stone, ND, Youngman, RR (1994) Generalist predator consumption of armyworm (Lepidoptera: Noctuidae) and effect of predator removal on damage in no-till corn. Environ. Entomol. 23: 617–622 CrossRef
Dutton A, Klein H, Romeis J, Bigler F (2002) Uptake of Bt-toxin by herbivores feeding on transgenic maize and consequences for the predator Chrysoperla carnea. Ecol. Entomol. 27: 441–447
French, BW, Chandler, LD, Ellsbury, MM, Fuller, BW, West, M (2004) Ground beetle (Coleoptera: Carabidae) assemblages in a transgenic corn-soybean cropping system. Environ. Entomol. 33: 554–563 CrossRef
Gugerli, P (1979) Le test immuno-enzymatique (ELISA) et son application pour le diagnostic rapide des viroses de la pomme de terre. Rev. Suisse Agric. 11: 253–260
Gugerli P (1986) Potato Viruses. In Bergmeier HU, ed, Methods of Enzymatic Analysis, VCH-Verlagsgesellschaft, Weinheim, vol XI, pp 430–446
Head, G, Brown, CR, Groth, ME, Duan, JJ (2001) Cry1Ab protein levels in phytophagous insects feeding on transgenic corn: implications for secondary exposure risk assessment. Entomol. Exp. Appl. 99: 37–45 CrossRef
Hladilek EE (2003) The role of ground beetles (Coleoptera: Carabidae) in detrital food webs. M.S. thesis, University of Minnesota, St. Paul
Howald R, Zwahlen C, Nentwig W (2003) Evaluation of Bt oilseed rape on the non-target herbivore Athalia rosae. Entomol. Exp. Appl. 106: 87–93
Ingerson-Mahar J (2002) Relating diet and morphology in adult carabid beetles. In Holland JM, ed, The Agroecology of Carabid Beetles, Intercept, Andover, pp 111–136
Kromp, B (1999) Carabid beetles in sustainable agriculture: A review on pest control efficacy, cultivation impacts and enhancement. Agr. Ecosyst. Environ. 74: 187–228 CrossRef
Lövei, GL, Sunderland, KD (1996) Ecology and behavior of ground beetles (Coleoptera: Carabidae). Annu. Rev. Entomol. 41: 231–256 CrossRef
Lozzia, GC, Rigamonti, IE (1998) Preliminary study on the effects of transgenic maize on non target species. IOBC-Integrated Control Cereal Crops 21: 171–180
Meissle, M, Vojtech, E, Poppy, GM (2005) Effects of Bt maize-fed prey on the generalist predator Poecilus cupreus L. (Coleoptera: Carabidae). Transgenic Res. 14: 123–132 CrossRef
Raps, A, Kehr, J, Gugerli, P, Moar, WJ, Bigler, F, Hilbeck, A (2001) Immunological analysis of phloem sap of Bacillus thuringiensis corn and of the nontarget herbivore Rhopalosiphum padi (Homoptera: Aphididae) for the presence of Cry1Ab. Mol. Ecol. 10: 525–533 CrossRef
Riddick, EW, Dively, G, Barbosa, P (1998) Effect of a seed-mix deployment of Cry3A-transgenic and nontransgenic potato on the abundance of Lebia grandis (Coleoptera: Carabidae) and Coleomegilla maculata (Coleoptera: Coccinellidae). Ann. Entomol. Soc. Am. 91: 647–653 CrossRef
Toft S, Bilde T (2002) Carabid diets and food value. In Holland JM, ed, The Agroecology of Carabid Beetles, Intercept, Andover, pp 81–110
Wandeler H, Bahylova J, Nentwig W (2002) Consumption of two Bt and six non-Bt corn varieties by the woodlouse Porcellio scaber. Basic Appl. Ecol. 3: 357–365
Zwahlen, C, Hilbeck, A, Gugerli, P, Nentwig, W (2003a) Degradation of the Cry1Ab protein within transgenic Bacillus thuringiensis corn tissue in the field. Mol. Ecol. 12: 765–775 CrossRef
Zwahlen C, Hilbeck A, Howald R, Nentwig W (2003b) Effects of transgenic Bt corn litter on the earthworm Lumbricus terrestris. Mol. Ecol. 12: 1077–1086