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Facile immobilization of lipase based on Pickering emulsion via a synergistic stabilization by palygorskite–enzyme

Published online by Cambridge University Press:  01 August 2019

Dong Li
Affiliation:
School of Chemical Engineering, Huaiyin Institute of Technology, Key Laboratory for Palygorskite Science and Applied Technology of Jiangsu Province, Huaian 223003, PR China
Min Shen
Affiliation:
School of Chemical Engineering, Huaiyin Institute of Technology, Key Laboratory for Palygorskite Science and Applied Technology of Jiangsu Province, Huaian 223003, PR China
Guofan Sun
Affiliation:
School of Chemical Engineering, Huaiyin Institute of Technology, Key Laboratory for Palygorskite Science and Applied Technology of Jiangsu Province, Huaian 223003, PR China
Huiran Jin
Affiliation:
School of Chemical Engineering, Huaiyin Institute of Technology, Key Laboratory for Palygorskite Science and Applied Technology of Jiangsu Province, Huaian 223003, PR China
Peng Cai
Affiliation:
School of Chemical Engineering, Huaiyin Institute of Technology, Key Laboratory for Palygorskite Science and Applied Technology of Jiangsu Province, Huaian 223003, PR China
Zhihui Wang
Affiliation:
School of Chemical Engineering, Huaiyin Institute of Technology, Key Laboratory for Palygorskite Science and Applied Technology of Jiangsu Province, Huaian 223003, PR China
Yeling Jin
Affiliation:
School of Chemical Engineering, Huaiyin Institute of Technology, Key Laboratory for Palygorskite Science and Applied Technology of Jiangsu Province, Huaian 223003, PR China
Jing Chen
Affiliation:
School of Chemical Engineering, Huaiyin Institute of Technology, Key Laboratory for Palygorskite Science and Applied Technology of Jiangsu Province, Huaian 223003, PR China
Shijie Ding*
Affiliation:
School of Chemical Engineering, Huaiyin Institute of Technology, Key Laboratory for Palygorskite Science and Applied Technology of Jiangsu Province, Huaian 223003, PR China
*

Abstract

A Pickering emulsion was prepared via synergistic stabilization of a lipase and palygorskite particles. The optimum conditions for the stabilization of the Pickering emulsion, such as the concentrations of the palygorskite particles and lipase, were explored. The morphology of emulsion droplets was examined using digital optical microscopy and polarizing optical microscopy. The palygorskite–lipase co-stabilized Pickering emulsions were investigated by determination of the adsorption rate, pH and zeta potential of the aqueous dispersion, as well as by determining the contact angle values of the lipase solution on a palygorskite disc that was immersed in toluene. The catalytic performance of the immobilized lipase in the Pickering emulsion was studied via the investigation of its thermal stability, storage stability and reusability. The immobilized lipase showed greater stability than the free lipase. The lipase immobilized by Pickering emulsion retained a high level of activity even after seven periods of recycling.

Type
Article
Copyright
Copyright © Mineralogical Society of Great Britain and Ireland 2019 

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Footnotes

Associate Editor: Chun-Hui Zhou

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