Skip to main content Accessibility help
×
Home

Isolation and sequence analysis of the floral homeotic gene BAP2 in Brassica rapa

  • Lu Guang-Yuan (a1), Wu Xiao-Ming (a1), Chen Bi-Yun (a1), Gao Gui-Zhen (a1) and Xu Kun (a1)...

Abstract

The Arabidopsis homeotic gene AP2 is essential for floral meristem development and organ specification. In this study, we report the isolation and sequence comparison of the AP2 homologue of Brassica rapa (BAP2). The results showed that the BAP2 DNA sequence was 2138 bp in length and contained nine introns. It shared 90% identity with AP2 in the coding region. The putative BAP2 polypeptide contained a nuclear localization signal (NLS) sequence and two copies of highly conserved AP2 domain, suggesting that BAP2 may function similarly to AP2. Two nucleotide variations were detected in DNA sequences from wild-type and apetalous B. rapa plants at the BAP2 locus, while the putative polypeptides were identical. We propose that BAP2 is not likely to be responsible for the apetalous mutation in B. rapa.

Copyright

Corresponding author

*Corresponding author. Email: wuxm@oilcrops.cn

References

Hide All
Bowman, JL, Alvarez, J, Weigel, D and Meyerowitz, EM (1991) Genetic interactions among floral homeotic genes of Arabidopsis. Development 112: 120.
Buzza, GC (1983) The inheritance of an apetalous flower character in canola (B. napus). Crucifer Newsletters 8: 1112.
Elliott, RC, Betzner, AS, Huttner, E, et al. (1996) AINTEGUMENTA, an APETALA2 -like gene of Arabidopsis with pleiotropic roles in ovule development and floral organ growth. The Plant Cell 8: 155168.
Fray, MJ, Evans, EJ, Lydiate, DJ and Arthur, A (1996) Physiological assessment of apetalous flowers and erectophile pods in oilseed rape (Brassica napus). Journal of Agricultural Sciences 127: 193200.
Fray, MJ, Puangsomlee, P, Goodrich, J, Coupland, G, Evans, EJ, Arthur, AE and Lydiate, DJ (1997) The genetics of stamenoid petal production in oilseed rape (Brassica napus) and equivalent variation in Arabidopsis thaliana. Theoretical and Applied Genetics 94: 731736.
Fu, TD, Yang, GS, Tu, JX and Ma, CZ (2001) The present and future of rapeseed production in China. In: Liu, HL and Fu, TD (editors) Proceedings of International Symposium on Rapeseed Science. New York: Science Press, pp. 35.
Jofuku, KD, Den Boer, BGW, Montagu, MV and Okamuro, JK (1994) Control of Arabidopsis flower and seed development by the homeotic gene APETALA2. The Plant Cell 6: 12111225.
Kunst, L, Klenz, JE, Martinez-Zapater, J and Haughn, GW (1989) AP2 gene determines the identity of perianth organs in flower of Arabidopsis thaliana. The Plant Cell 1: 11951208.
Liu, YG, Mitsukawa, N, Oosumi, T and Whittier, RF (1995). Efficient isolation and mapping of Arabidopsis thaliana T-DNA insert junctions by Thermal Asymmetric Interlaced PCR. The Plant Journal 8: 457463.
Lu, GY, Yang, GY and Fu, TD (2003) An effective SSR detecting protocol in rapeseed. Chinese Journal of Oil Crop Sciences 25: 7981.
Lu, ZJ and Fu, SZ (1990) Inheritance of apetalous character in rape (Brassica napus L.) and its implication in breeding. Jiangshu Journal of Agricultural Sciences 6: 3036.
Maes, T, Van de Steene, N, Zethof, J, Karimi, M, D'Hauw, M, Mares, G, et al. (2001) Petunia AP2-like genes and their role in flower and seed development. The Plant Cell 13: 229244.
Mitchell, PJ and Tjian, R (1989) Transcriptional regulation in mammalian cells by sequence-specific DNA binding proteins. Science 245: 371378.
Moose, SP and Sisco, PH (1996) Glossy15, an APETALA2 -like gene from maize that regulates leaf epidermal cell identity. Genes and Development 10: 30183027.
Ohme-Takagi, M and Shinshi, H (1995) Ethylene-inducible DNA binding proteins that interact with an ethylene-responsive element. The Plant Cell 7: 173182.
Okamuro, JK, Caster, B, Villarroel, R, Van Montagu, M and Jofuku, KD (1997) The AP2 domain of APETALA2 defines a large new family of DNA binding proteins in Arabidopsis. Proceedings of the Natural Academy Sciences of the USA 94: 70767081.
Riechmann, JL and Meyerowitz, EM (1998) The AP2/EREBP family of plant transcription factors. Biological Chemistry 379: 633646.
Singh, D (1961) An apetalous mutant in Toria brown sarson (Brassica campestris var. Brown sarson) and its inheritance. Current Sciences 30: 6263.
Vahala, T, Oxelman, B and von Arnold, S (2001) Two APETALA2 -like genes of Picea abies are differentially expressed during development. Journal of Experimental Botany 52: 11111115.
Varagona, MJ, Schmidt, RJ and Raikhel, NV (1992) Nuclear localization signals required for nuclear targeting of the maize regulatory protein opaque-2. The Plant Cell 4: 12131227.
Weigel, D and Meyerowitz, E (1994) The ABCs of floral homeotic genes. Cell 78: 203209.
Wu, XM, Wang, HZ, Zheng, PY, Chen, BY, Xu, K and Li, XZ (2001) Morphological characterization of the flower organ of a B. rapa apetalous mutant. Chinese Journal of Oil Crop Sciences 2: 6869.

Keywords

Isolation and sequence analysis of the floral homeotic gene BAP2 in Brassica rapa

  • Lu Guang-Yuan (a1), Wu Xiao-Ming (a1), Chen Bi-Yun (a1), Gao Gui-Zhen (a1) and Xu Kun (a1)...

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed