Hostname: page-component-76fb5796d-r6qrq Total loading time: 0 Render date: 2024-04-26T06:35:59.262Z Has data issue: false hasContentIssue false
  • English
  • Français

Composition and changes of yolk proteins from silkworm Bombyx mori during embryonic development stages

Published online by Cambridge University Press:  13 June 2008

Yan Xin-Pei ,
Zhong Bo-Xiong ,
Xu Meng-Kui ,
Yao Guo-Hua ,
Chen Qi-Long  and
Tong Xiao-Fen
Yan Xin-Pei
Affiliation:
Department of Bioresource Science, College of Animal Science, Zhejiang University, Hangzhou 310029, China
Zhong Bo-Xiong*
Affiliation:
Department of Bioresource Science, College of Animal Science, Zhejiang University, Hangzhou 310029, China
Xu Meng-Kui
Affiliation:
Department of Bioresource Science, College of Animal Science, Zhejiang University, Hangzhou 310029, China
Yao Guo-Hua
Affiliation:
Department of Bioresource Science, College of Animal Science, Zhejiang University, Hangzhou 310029, China
Chen Qi-Long
Affiliation:
Department of Bioresource Science, College of Animal Science, Zhejiang University, Hangzhou 310029, China
Tong Xiao-Fen
Affiliation:
Department of Bioresource Science, College of Animal Science, Zhejiang University, Hangzhou 310029, China
*
*Corresponding author. Email: bxzhong@zju.edu.cn
Get access Rights & Permissions [Opens in a new window]

Abstract

The yolk proteins of the silkworm, Bombyx mori, are important sources of nutrition and energy during embryo development. By analysing the yolk proteins from silkworm eggs, the composition and the metabolism of yolk proteins during embryo development stages can be studied at the proteome level. Yolk proteins were obtained from unfertilized eggs and eggs at different embryo development stages by dissection and then separated and analysed by two-dimensional electrophoresis and its corresponding image analysis system. It was discovered that over 200 proteins exist in mature silkworm eggs. Some specific spots of yolk proteins were found in different silkworm strains, while few differences in yolk proteins appeared during different development stages in the same silkworm strain. This result indicates that the silkworm embryo does not utilize yolk proteins selectively by absorbing one or some special proteins, but by absorbing the yolk protein grains one by one during development.

Keywords

silkworm Bombyx moriembryo developmentyolk proteintwo-dimensional electrophoresisprotein pattern
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 2 , Issue 2 , August 2005 , pp. 99 - 106
Copyright
Copyright © China Agricultural University and Cambridge University Press 2005

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bradford, MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Analytical Biochemistry 72: 248–254.CrossRefGoogle ScholarPubMed
Chen, YL and Yamashita, O (1990) Non-selective uptake of 30 kDa plasma proteins by developing ovaries of the silkworm, Bombyx mori. Journal of Sericulture Science of Japan 59: 202–209.Google Scholar
Inagami, K (1954) Studies on the proteins of the body fluid in the silkworm: on the electrophoretic components of the body fluid proteins. Journal of Sericulture Science of Japan 23: 304–307.Google Scholar
Indrasith, LS, Sasaki, T and Yamashita, O (1988) A unique protease responsible for selective degradation of a yolk protein in Bombyx mori. Purification, characterization and cleavage profile. Journal of Biological Chemistry 263: 1045–1051.CrossRefGoogle ScholarPubMed
Irie, K and Yamashita, O (1983) Egg-specific protein in the silkworm, Bombyx mori: purification, properties, localization and titer changes during oogenesis and embryogenesis. Insect Biochemistry 13: 71–80.CrossRefGoogle Scholar
Izumi, S, Tomino, S and Chino, H (1980) Purification and molecular properties of vitellin from the silkworm, Bombyx mori. Insect Biochemistry 10: 199–208.CrossRefGoogle Scholar
Izumi, S, Kiguchi, K and Tomino, S (1984) Hormonal regulation of biosynthesis of major plasma proteins in Bombyx mori. Zoological Science 1: 223–228.Google Scholar
Maki, N and Yamashita, O (1997) Purification and characterization of a protease degrading 30 k Da yolk proteins of the silkworm, Bombyx mori. Insect Biochemistry and Molecular Biology 27: 721–728.CrossRefGoogle Scholar
Mine, E, Izumi, S, Katsuki, M and Tomino, S (1983) Development and sex-dependent regulation of storage protein synthesis in the silkworm, Bombyx mori. Developmental Biology 97: 329–337.CrossRefGoogle ScholarPubMed
Pan, ML, Bell, WJ and Telfer, WH (1969) Vitellogenic blood protein synthesis by insect fat body. Science 165: 393–394.CrossRefGoogle ScholarPubMed
Sakai, N, Mori, S, Izumi, S, Haino-Fukushima, K, Ogura, T, Maekawa, H et al. , (1988) Structures and expression of mRNAs coding for major plasma proteins of Bombyx mori. Biochimica et Biophysica Acta 949: 224–232.CrossRefGoogle ScholarPubMed
Sanchez, JC, Rouge, V, Pisteur, M, Ravier, F, Tonella, L, Moosmayer, M et al. , (1997) Improved and simplified in-gel sample application using reswelling of dry immobilized pH gradients. Electrophoresis 18: 324–327.CrossRefGoogle ScholarPubMed
Sato, Y and Yamashita, O (1991) Synthesis and secretion of egg-specific protein from follicle cells of the silkworm, Bombyx mori. Insect Biochemistry 21: 233–238.CrossRefGoogle Scholar
Wu, XF and Xu, JL (1991) The volume and physiological function of yolk protein in silkworm. Bulletin of Sericulture 3: 40–42 (in Chinese).Google Scholar
Zhan, XQ, Guan, YJ, Li, C, Chen, ZC, Xie, JY, Chen, P et al. , (2002) Differential proteomic analysis of human lung adenocarcinoma cell line A-549 and of normal cell line HBE. Acta Biochimica et Biophysica Sinica 34: 50–56 (in Chinese).Google ScholarPubMed
Zhong, BX (1997) A new method for analysis of rice protein using two-dimensional electrophoresis. Journal of Zhejiang Agricultural University 23: 128–132 in Chinese.Google Scholar
Zhong, BX (1999) Characterization and stage-specific change of proteins during the embryonic development of silkworm Bombyx mori. Acta Genetica Sinica 26: 627–633 (in Chinese).Google ScholarPubMed
Zhong, BX, Karibe, H, Komatsa, S, Ichimura, H, Nagamura, Y, Sasaki, T et al. , (1997) Screening of rice genes from a cDNA catalog based on the sequence data-file of proteins separated by two-dimensional electrophoresis. Breeding Science 47: 245–251.Google Scholar
Zhong, BX, Weng, HB and Fang, WH (2002) Preparation of protein samples for gel electrophoresis by sequential extraction. Journal of Zhejiang University Science 5: 606–610.Google Scholar
Zhu, J and Dai, YJ (1989) The progress of yolk protein molecular biology in silkworm. Foreign Agriculture–Sericulture 3: 4–11 (in Chinese).Google Scholar
Zhu, J, Indrasith, L and Yamashita, O (1986) Characterization of vitellin, egg-specific protein and 30 k Da protein from Bombyx eggs and their fates during oogenesis and embryogenesis. Biochimica et Biophysica Acta 882: 427–436.CrossRefGoogle Scholar