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Characterization of trait variations in progenies of Gossypium hirsutum transformed with the genomic DNA of Gossypium barbadense

  • Du Xiong-Ming (a1), Pan Zhao-E (a1), Sun Jun-Ling (a1), Zhou Zhong-Li (a1) and Pang Bao-Yin (a1)...

Abstract

Four variant lines, HB1, HB2, HB3 and HB4, were derived from progenies of upland cotton (Gossypium hirsutum) cultivar Yumian 17 that had been transformed with genomic DNA from sea island cotton, Gossypium barbadense, using the pollen tube pathway (PTP) method. They showed significant differences from the wild-type recipient in terms of fibre quality, lint percentage, boll weight and other agronomic traits. The four variant lines also differed from the wild-type recipient in the amplification products of two SSR loci, indicating that the DNA of G. barbadense had been introduced into the genome of the recipient and was inherited stably. On the other hand, the agronomic traits of another three lines (HB5, HB6 and HB7) derived from the same transformation experiment were identical to those of the wild-type recipient, indicating that G. barbadense DNA had not integrated into the genome of these lines. A combination of the analyses on agronomic traits and SSR markers indicated that transgenic upland cotton strains produced by the PTP method could become homozygous in only a few generations and the foreign DNA introduced during the transformation experiments could be inherited stably in the transgenic progenies.

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*Corresponding author: Email: dxm630723@163.com

References

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Czernilofsky, AP, Hain, R, Herrera-Estrella, L, Lorz, H, Goyvaerts, E, Baker, BJ et al. , (1986) Fate of selectable marker DNA integrated into the genome of Nicotiana tabacum. DNA 5: 101113.
Duan, XF and Chen, SB (1985) Character variation of transformation exogenous DNA in rice. Scientia Agricultura Sinica 3: 610 (in Chinese with English abstract).
Fu, J, Yan, QQ and Xu, QG (1990) A simple way of introduction of exogenous DNA and induce variation in rice. Hunan Agricultural Science 2: 1820 (in Chinese with English abstract).
Huang, JQ, Qian, SY, Liu, GL, Weng, J, Zheng, YS and Zhou, GY (1981) Variations in the characters of upland cotton (Gossypium hirsutum) induced by exotic DNA of sea-island cotton (Gossypium barbadense). Acta Genetica Sinica 8(1): 5662 (in Chinese with English abstract).
Huang, JQ, Qian, SY, Liu, GL, Weng, J, Zheng, YS and Zhou, GY (1982) Variations in the characters of Gossypium arboreum induced by exotic DNA of sea-island cotton (Gossypium barbadense). Jiangsu Agricultural Science 11: 1821 (in Chinese with English abstract).
Jia, J (1996) Molecular germplasm diagnostics and molecular marker-assisted breeding. Scientia Agricultura Sinica 29(4): 110 (in Chinese with English abstract).
Kohli, A, Griffiths, S, Palacios, N, Twyman, RM, Vain, P, Laurie, DA et al. , (1999) Molecular characterization of transforming plasmid rearrangements in transgenic rice reveals a recombination hotspot in the CaMV 35S promoter and confirms the predominance of microhomology-mediated recombination. Plant Journal 17: 591601.
Paterson, AH, Brubaker, CL and Wendel, JF (1993) A rapid method for extraction of cotton genomic DNA suitable for RFLP or PCR analysis. Plant Molecular Biology Report 11(2): 122127 (in Chinese with English abstract).
Singh, RJ, Klein, TM, Manvais, CJ, Knowlton, S, Hymowitz, T and Kostow, CM (1998) Cytological characterization of transgenic soybean. Theoretical and Applied Genetics 96: 319324.
Wang, SC, Wang, GY, Ding, QX, Zhang, H, Xie, YJ and Dai, JR (1999) Studies of transgenic segregation and integration in maize. Acta Genetica Sinica 26(3): 254261 (in Chinese with English abstract).
Weng, J, Shen, WF, Wang, ZF, Chen, KQ, Yang, WX, Gong, ZZ et al. , (1984) Molecular proof of exogenous DNA transformation to cotton. Acta Biochemica and Biophysica 16(3): 325327 (in Chinese with English abstract).
Wu, X (1984) Research on variated effects of Gossypium hirsutum induced by multiple exogenous DNA. Hunan Agricultural Science 73(4): 57 (in Chinese with English abstract).
Xia, LQ and Guo, SD (2001) Integration and genetic stability of BT gene in the transgenic cotton with double pest-resistant genes. Chinese Science Bulletin 46(7): 565568 (in Chinese with English abstract).
Xinjian Production and Construction Corps (1999) Cotton germplasm enhancement by transformation of exogenous DNA. Crop Genetic Resources 69(3): 45 (in Chinese with English abstract).
Zhang, J, Wu, YT, Guo, WZ and Zhang, TZ (2000) Fast screening of microsatellite markers in cotton with PAGE/silver staining. Cotton Science 12(5): 267269 (in Chinese with English abstract).
Zheng, HJ, Yu, YJ, Yin, CY, Zhang, LX and Li, B (2002) Identification of dwarf variants of wheat by RAPD method and their genetics analysis. Acta Botanica Boreali-Occidentalia Sinica 22(4): 758765 (in Chinese with English abstract).
Zhou, GY, Weng, J, Huang, JQ, Zheng, YS, Qian, SY and Liu, GL (1983a) Exogenous DNA mediated phenotypic variation in cotton. In: Goldberg, RB (editor) Plant Molecular Biology. New York: Alan R Liss.
Zhou, G, Weng, J, Zeng, Y, Huang, J, Qian, S and Liu, G (1983b) Introduction of exogenous DNA into cotton embryos. Methods in Enzymology 101: 433481.

Keywords

Characterization of trait variations in progenies of Gossypium hirsutum transformed with the genomic DNA of Gossypium barbadense

  • Du Xiong-Ming (a1), Pan Zhao-E (a1), Sun Jun-Ling (a1), Zhou Zhong-Li (a1) and Pang Bao-Yin (a1)...

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