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Identification of resistance to corn-borer of transgenic maize inbred lines and hybrids with GFM Cry1A gene

Published online by Cambridge University Press:  30 October 2009

Yuan Ying ,
Li Xiao-Hui ,
Kong Xiang-Mei ,
Jiang Zhi-Lei ,
Lin Chun-Jing ,
Li Cong-Cong ,
Liu Na ,
Sun Chuan-Bo  and
Liu De-Pu
Yuan Ying
Affiliation:
Center of Agri-biotechnology, Jilin Academy of Agricultural Sciences, Changchun 130124, China
Li Xiao-Hui
Affiliation:
Center of Agri-biotechnology, Jilin Academy of Agricultural Sciences, Changchun 130124, China
Kong Xiang-Mei
Affiliation:
Center of Agri-biotechnology, Jilin Academy of Agricultural Sciences, Changchun 130124, China
Jiang Zhi-Lei
Affiliation:
Center of Agri-biotechnology, Jilin Academy of Agricultural Sciences, Changchun 130124, China
Lin Chun-Jing
Affiliation:
Center of Agri-biotechnology, Jilin Academy of Agricultural Sciences, Changchun 130124, China
Li Cong-Cong
Affiliation:
Center of Agri-biotechnology, Jilin Academy of Agricultural Sciences, Changchun 130124, China
Liu Na
Affiliation:
Center of Agri-biotechnology, Jilin Academy of Agricultural Sciences, Changchun 130124, China
Sun Chuan-Bo
Affiliation:
Center of Agri-biotechnology, Jilin Academy of Agricultural Sciences, Changchun 130124, China
Liu De-Pu*
Affiliation:
Center of Agri-biotechnology, Jilin Academy of Agricultural Sciences, Changchun 130124, China
*
*Corresponding author. E-mail: liudp@cjaas.com
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Abstract

Six transgenic maize lines with the fully modified gene GFM Cry1A were obtained and polymerase chain reaction (PCR) analysis of their T5 generation plants indicated that foreign genes could be stably inherited. Three hybrids (Simi25Bt, Tongdan24Bt and Jidan209Bt) were created using the transgenic lines and combined with regular maize inbred lines. The results of resistance identification of the transgenic inbred lines and hybrids showed that the difference of resistance among transgenic lines was very significant; there also existed a difference among individuals of the same line and among the three hybrids. Compared with the control inbred line (CK), the average of four transgenic inbred lines in the number of tunnels per stalk, larval tunnel length per plant and length of surviving larvae were decreased by 70%, 80% and 70%, respectively. Enzyme-linked immunosorbent assay (ELISA) testing showed that the average of Bt Cry1A protein expression level for six transgenic inbred lines was 0.16% total protein and the highest expression level was 0.19%. At maturity, compared with the control variety (CK), the larval tunnel length per plant of the three hybrids (Simi25Bt, Tongdan24Bt and Jidan209Bt) was decreased by 39.97%, 36.20% and 53.83%, respectively, which was a decrease of 43.36% on average. The investigation of agronomic characters showed that there was no significant difference between the improved hybrids and the control in plant height, ear length, row number per ear, kernel number per row and 100-kernels weight. It is thought that the GFM Cry1A gene can be applied to improve resistance to corn-borer, and maize inbred lines with the Bt gene can be directly applied to conventional maize breeding.

Keywords

maizeGFM Cry1A geneinbred linehybrid
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 6 , Issue 2 , August 2009 , pp. 177 - 182
Copyright
Copyright © China Agricultural University 2009

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References

Ding, QX, Xie, YJ, Dai, JR, et al. (1993) Introducing Bt crystal protein gene into maize with various injections. Science in China (Series B) 23(7): 707–713 (in Chinese).Google Scholar
He, KL, Wang, ZY, Zhou, DR, Wen, LP and Song, YY (2000) Methodologies and criteria for evaluating maize resistance to Asian maize borer. Journal of Shenyang Agricultural University 31(5): 439–443 (in Chinese with English abstract).Google Scholar
Koziel, MG, Belad, GL, Brown, C, et al. (1993) Field performance of elite transgenic maize plants expressing an insecticidal protein derived from Bacillus thuringiensis. Bio/Technology 11: 194–200.Google Scholar
Liu, ZH, Tang, JH, Li, BJ, et al. (2003) Primary study on identification of corn-borer resistance in corn inbred-lines transferred with Bt gene. Acta Agronomica Sinica 29(4): 621–625 (in Chinese with English abstract).Google Scholar
Ni, WC, Zhang, ZL and Guo, SD (1998) Development of transgenic insect-resistant cotton plants. Scientia Agricultura Sinica 2: 8–13 (in Chinese with English abstract).Google Scholar
Wang, GL and Fang, HJ (2002) Plant Genetic Engineering, 2nd ed.Beijing: Science Press (in Chinese).Google Scholar
Wang, GY, Du, TB, Zhang, H, et al. (1995) Regeneration of transgenic plants and transformation of Bt gene by particle bombardment. Science in China (Series B) 25(1): 71–76 (in Chinese).Google Scholar
Wang, SC, Wang, GY, Ding, QX, Zhang, H, Xie, YJ and Dai, JR (1999) Studies of transgenic segregation and integration in maize. Acta Genetic Sinica 26: 254–261 (in Chinese with English abstract).Google ScholarPubMed
Yuan, Y, Liu, DP, Zheng, PH, Chen, YB and Wen, G (2001) Inducement on embryo callus of maize inbred lines in Northeast China. Journal of Maize Sciences 9(1): 37–38 (in Chinese with English abstract).Google Scholar
Yuan, Y, Li, XH, Kong, XM, et al. (2007) Developing Bt maize inbred line through transforming Bt CryIA gene. Molecular Plant Breeding 5(4): 572–576 (in Chinese with English abstract).Google Scholar
Zhang, HW, Zhang, HM, Zhang, ZP, Chen, G, Wang, GY and Tan, ZB (2004) Production of Bt transgenic maize resistant to corn borer. Acta Botanica Boreali-Occidentalia Sinica 24(7): 1266–1270 (in Chinese with English abstract).Google Scholar
Zhou, DR and He, KL (1995) Integrated Pest Management (IPM) measures for the prevention and cure of Ostrinia furnacalis. Beijing: Golden Shield Press (in Chinese).Google Scholar
Zhu, WH, Wang, QD and Liu, ZH (2004) Identification of insect resistance and inheritance analysis of transgenic Bt gene in maize. Journal of Henan Agricultural University 38(2): 135–138 (in Chinese with English abstract).Google Scholar