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Expressed sequence tag analysis and cDNA array establishment of Nicotiana tabacum: application to salinity stress

Published online by Cambridge University Press:  24 April 2009

Li Wen-Zheng
Affiliation:
Yunnan Tobacco Research Institute, Yuxi 653100, China
Song Li-Min
Affiliation:
Yunnan Tobacco Research Institute, Yuxi 653100, China
Li Yong-Ping
Affiliation:
Yunnan Tobacco Research Institute, Yuxi 653100, China
Lu Xiu-Ping
Affiliation:
Yunnan Tobacco Research Institute, Yuxi 653100, China
Luo Hong-Mei
Affiliation:
College of Life and Environmental Science, Hangzhou Teachers University, Hangzhou 310036, China
Dai Cheng-En
Affiliation:
Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China
Fang Yong-Qi
Affiliation:
Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China
Dong Hai-Tao
Affiliation:
Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China
Li De-Bao*
Affiliation:
Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China
*
*Corresponding author. E-mail: Lidb@mail.hz.zj.cn

Abstract

This study aimed to explore high-throughput cDNA array monitoring technology and to apply it to the gene expression spectrum analysis of salinity-challenged tobacco plants. A Nicotiana tabacum cDNA library was sequenced and found to consist of 5927 high-quality sequences (GenBank accession nos CV015900-CV021826). By analysing the expressed sequence tags (ESTs), the proportion of N. tabacum genes was identified at the EST level. A cDNA array was constructed based on the tentative unique transcripts (TUTs) derived from EST assembling results. A total of 42 differentially expressed genes were identified, including plasma membrane intrinsic protein 2a, ethylene-responsive proteinase and pre-mRNA splicing factor prp31 gene, suggesting that there was a complicated biological response in N. tabacum under saline stress.

Type
Research Papers
Copyright
Copyright © China Agricultural University 2009

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References

Altschul, SF, Gish, W, Miller, W, Myers, EW and Lipman, DJ (1990) Basic local alignment search tool. Journal of Molecular Biology 215: 403410.CrossRefGoogle ScholarPubMed
Apweiler, R, Bairoch, A, Wu, CH, et al. (2004) UniProt: the Universal Protein knowledgebase. Nucleic Acids Research 32: D115D119.CrossRefGoogle Scholar
Ast, G (2004) How did alternative splicing evolve? Nature Reviews Genetics 5: 773782.Google Scholar
Bohnert, HJ, Ayoubi, P, Borchert, C, et al. (2001) A genomics approach towards salt stress tolerance. Plant Physiology and Biochemistry 39: 295311.Google Scholar
Bonnet, P, Bourdon, E, Ponchet, M, Blein, JP and Ricci, P (1996) Acquired resistance triggered by elicitins in tobacco and other plants. European Journal of Plant Pathology 102: 181192.Google Scholar
Ewing, B, Hillier, L, Wendl, MC and Green, P (1998) Base-calling of automated sequencer traces using Phred. I. Accuracy assessment. Genome Research 8: 175185.Google Scholar
Hewitt, EJ (1965) Sand and Water Culture Methods Used in the Study of Plant Nutrition. Beijing: Science Press, pp. 225227 (in Chinese).Google Scholar
Honée, G, Melchers, LS, Vleeshouwers, VGAA, van Roekel, JSC and de Wit, PJGM (1995) Production of the AVR9 elicitor from the fungal pathogen Cladosporium fulvum in transgenic tobacco and tomato plants. Plant Molecular Biology 29: 909920.Google Scholar
Huang, X and Madan, A (1999) CAP3: A DNA sequence assembly program. Genome Research 9: 868877.Google Scholar
Kazan, K (2003) Alternative splicing and proteome diversity in plants: The tip of the iceberg has just emerged. Trends in Plant Science 8: 468471.Google Scholar
Keller, H, Pamboukdjian, N, Poncbet, M, et al. (1999) Pathogen-induced elicitin production in transgenic tobacco generates a hypersensitive response and nonspecific disease resistance. Plant Cell 11: 223235.Google Scholar
Mewes, HW, Frishman, D, Guldener, U, et al. (2002) MIPS: A database for genomes and protein sequences. Nucleic Acids Research 30: 3134.Google Scholar
Ozturk, ZN, Talam'e, V, Deyholos, M, et al. (2002) Monitoring large-scale changes in transcript abundance in drought- and salt-stressed barley. Plant Molecular Biology 48: 551573.Google Scholar
Pertea, G, Huang, X, Liang, F, et al. (2003) TIGR gene indices clustering tools (TGICL): A software system for fast clustering of large EST datasets. Bioinformatics 19: 651652.Google Scholar
Pieterse, CMJ and van Loon, LC (1999) Salicylic acid-independent plant defence pathways. Trends in Plant Science 4: 5258.Google Scholar
Sambrook, J and Russell, DW (2001) Molecular Cloning, 3rd ed.New York: Cold Spring Harbor Laboratory Press.Google Scholar
Shinozaki, K and Yamaguchi-Shinozaki, K (2000) Molecular responses to dehydration and low temperature: differences and cross-talk between two stress signaling pathways. Current Opinion in Plant Biology 3: 217222.Google Scholar
Staskawicz, BJ, Ausubel, FM and Baker, BJ (1995) Molecular genetics of plant disease resistance. Science 268: 661667.Google Scholar
Trail, F, Xu, JR, Miguel, PS, Halgren, RG and Kistler, HC (2003) Analysis of expressed sequence tags from Gibberella zeae (Anamorpha Fusarium graminearum). Fungal Genetics and Biology 38: 187197.Google Scholar
Yamada, S, Katsuhara, M, Kelly, WB, Michalowski, CB and Bohnert, HJ (1995) A family of transcripts encoding water channel proteins: tissue-specific expression in the common ice plant. Plant Cell 7: 11291142.Google Scholar
Zhu, JK (2001) Plant salt tolerance. Trends in Plant Science 6: 6671.Google Scholar