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Expressed sequence tag analysis and cDNA array establishment of Nicotiana tabacum: application to salinity stress

Published online by Cambridge University Press:  24 April 2009

Li Wen-Zheng
Yunnan Tobacco Research Institute, Yuxi 653100, China
Song Li-Min
Yunnan Tobacco Research Institute, Yuxi 653100, China
Li Yong-Ping
Yunnan Tobacco Research Institute, Yuxi 653100, China
Lu Xiu-Ping
Yunnan Tobacco Research Institute, Yuxi 653100, China
Luo Hong-Mei
College of Life and Environmental Science, Hangzhou Teachers University, Hangzhou 310036, China
Dai Cheng-En
Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China
Fang Yong-Qi
Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China
Dong Hai-Tao
Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China
Li De-Bao*
Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China
*Corresponding author. E-mail:


This study aimed to explore high-throughput cDNA array monitoring technology and to apply it to the gene expression spectrum analysis of salinity-challenged tobacco plants. A Nicotiana tabacum cDNA library was sequenced and found to consist of 5927 high-quality sequences (GenBank accession nos CV015900-CV021826). By analysing the expressed sequence tags (ESTs), the proportion of N. tabacum genes was identified at the EST level. A cDNA array was constructed based on the tentative unique transcripts (TUTs) derived from EST assembling results. A total of 42 differentially expressed genes were identified, including plasma membrane intrinsic protein 2a, ethylene-responsive proteinase and pre-mRNA splicing factor prp31 gene, suggesting that there was a complicated biological response in N. tabacum under saline stress.

Research Papers
Copyright © China Agricultural University 2009

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