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Cloning and tissue expression of the adiponectin gene in geese

Published online by Cambridge University Press:  30 October 2009

Xu Guo-Qing
Affiliation:
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
Gong Dao-Qing*
Affiliation:
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
Chu Dong-Sheng
Affiliation:
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
Dong Biao
Affiliation:
National Water Fowl Germplasm Resource Pool, Taizhou 225300, China
Meng He
Affiliation:
College of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China
*
*Corresponding author. E-mail: yzgong@163.com

Abstract

Three pairs of primers were designed for amplification of the adiponectin gene in geese (Anser demeatica) based on the published sequences of chicken, duck and other adiponectin homologues. The complete 1062 bp sequence of the adiponectin gene in geese, obtained by polymerase chain reaction (PCR) amplification and splicing, contained two exons and one intron. The coding sequence was 738 bp long and included codes for 245 amino acids (GenBank Accession No. EU370686). The coding sequence and the corresponding protein of goose adiponectin shared the highest homology with ducks, 94.85% and 95.51%, respectively; homology with chicken adiponectin was next highest, while the lowest homology was to be found with mammals. The predicted molecular weight (MW) and isoelectric point (pI) were 26,603.3 Da and pH 5.19, respectively, which were highly similar to those of species considered above. Reverse transcription-PCR revealed that goose adiponectin mRNA was highly expressed in skeletal muscle, fatty tissue, heart and stomach muscles. Medium levels of expression were detected in the small intestine, stomach glandular tissue, kidney and lung, while it was only weakly expressed in the liver, spleen, ovaries and diencephalon.

Type
Review Article
Copyright
Copyright © China Agricultural University 2009

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