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Chromosomes of Cichlasoma trimaculatum (male), Heros managuense (female) and their hybrid offspring

Published online by Cambridge University Press:  29 January 2010

Chen You-Ling*
Affiliation:
College of Life Sciences, Fujian Normal University, Fuzhou 350108, China
Zhang Qiu-Jin
Affiliation:
College of Life Sciences, Fujian Normal University, Fuzhou 350108, China
Wang Yan-Yin
Affiliation:
College of Life Sciences, Fujian Normal University, Fuzhou 350108, China
Mao Jian-Ping
Affiliation:
College of Life Sciences, Fujian Normal University, Fuzhou 350108, China
*
*Corresponding author. E-mail: chenyouling2000@126.com

Abstract

The F1 hybrid offspring of the distant hybridization between Cichlasoma trimaculatum (male) and Heros managuense (female) were successfully obtained through artificial fertilization, and the chromosome specimen of kidney cells of the two parents and their offspring were prepared using a low colchicine concentration air drying method, Howell and Black's (1980) silver–nucleolar organizer region (Ag–NOR) staining method and Sumner's (1972) C-band staining method. The karyotypes, Ag–NORs and C-bands of C. trimaculatum, H. managuense and their offspring were observed. The results showed that the diploid numbers of the two parents and the F1 hybrid offspring were all 2n=48. For C. trimaculatum, the karyotype formula was 2n=2m+8sm+26st+12t, NF=58. Two Ag–NORs were seen on the tips of the short arm of chromosome st7, and the entire chromosomes of st9 and st13 and the centromeres of the other chromosomes had positive C-bands. For H. managuense, the karyotype formula was 2n=2m+6sm+28st+12t, NF=56. Two Ag–NORs were located on the tips of the short arm of chromosome sm2 and positive C-bands were found in the centromere of all chromosomes. In the F1 hybrid offspring, the chromosome formula was 2n=2m+8sm+30st+8t, NF=58. Two Ag–NORs were seen on the tips of the short arm of chromosome sm2; all chromosomes presented positive C-bands in the centromere. No heteromorphic sex chromosome was found. After comparison with the two parents, the F1 hybrid offspring was suggested to be the filial generation of the two hybrid parents rather than the gynogenesis of H. managuense induced by heterogeneous sperm of C. trimaculatum.

Type
Research Papers
Copyright
Copyright © China Agricultural University 2009

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References

Cao, LP, Ding, WD, Jia, YY, Xia, DQ and Wu, TT (2006) Analysis of chromosome karyotypes of Oreochromis aurea (female), Siniperca chuatsi (male) and their offspring. Journal of Agricultural Biotechnology 14: 187190 (in Chinese).Google Scholar
Chang, ZJ and Yu, QX (1993) Studies on the Ag-stained karyotypes of Cyprinus carpio and Carassius auratus. Wuhan University Journal of Natural Sciences 3: 110114 (in Chinese).Google Scholar
Chen, YL, Wang, YY, Wu, WS and Xiao, WY (2005) Analysis of chromosome complement C-banding and Ag-NORs of Astronotus ocellatus and Pterophyllum scalare. Chinese Journal of Zoology 40: 8490 (in Chinese).Google Scholar
Desprez, D, Melard, C, Hoareau, MC, Bellemene, Y, Bosc, P and Baroiller, JF (2003) Inheritance of sex in two ZZ pseudofemale lines of tilapia Oreochromis aureus. Aquaculture 218: 131140.CrossRefGoogle Scholar
Howell, WM and Black, G (1980) Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. Experientia 36: 10141015.CrossRefGoogle ScholarPubMed
Ji, XS, Chen, SL, Zhao, Y and Deng, H (2005) Cryopreservation of stone flounder and Japanese flounder sperms and its application to the breeding. Marine Fisheries Research 26: 1316 (in Chinese).Google Scholar
Levan, AD (1964) Nomenclature for centromeric position on chromosomes. Hereditas 52: 201220.CrossRefGoogle Scholar
Lin, YH (1982) A PHA injection method in vivo for the rapid obtainment of large numbers of metaphase figures from kidney cells of teleosts. Journal of Fisheries of China 6: 201204 (in Chinese).Google Scholar
Lin, YS, Li, BL, Cui, XS and Cao, WQ (2001) Hybridization breeding between Sparus macrocephalus and Sparus berda. Marine Fisheries 23: 6970 (in Chinese).Google Scholar
Lou, YD (1999) Fish Breeding. Beijing: China Agricultural Press (in Chinese).Google Scholar
Ma, L, Wang, J, Chen, WG, et al. (2002) Study on embryonic development of two drum fish crossbreeding filial generation I. Journal of Xiamen University (Natural Science Edition) 41: 378382 (in Chinese).Google Scholar
Ou, YJ, Li, JE and Zhou, HT (2000) Sparidae fishes are distant hybridization between the development and growth of larval development and growth of intergeneric crossing of Sparidae fishes. Journal of Fishery Sciences of China 7: 110112 (in Chinese).Google Scholar
Sumner, AT (1972) A simple technique for demonstrating centromeric heterochromatin. Experimental Cell Research 75: 304306.CrossRefGoogle ScholarPubMed
Wang, JL, Fang, GL and Chen, MR (2002) Variety appreciation of Flower Horn. In Li, XF (ed.) The Special Edition of Flower Horn. Taibei: Ornamental Fish Magazine. pp. 1625 (in Chinese).Google Scholar
Wang, XC, You, F, Ni, GT, Zhang, QX and Li, SY (2003) Hybridization between stone flounder Kareius bicoloratus and Olive Flounder Paralichthys olivaceus. Marine Sciences 27: 14 (in Chinese).Google Scholar
Wang, ZX, Zhang, JX and Jin, GQ (1986) Study on the incompatibility of fish hybridization. Acta Hydrobiologica Sinica 10: 171179 (in Chinese).Google Scholar
Xiao, P and Chen, DY (2002) Research progress and prospects of hybridization between crucian and carp in China. Acta Agriculturae Jiangxi 14: 5660 (in Chinese).Google Scholar
Yang, D and Yu, LN (2006) Sex and sex identification of fish. Acta Hydrobiologica Sinica 30: 221226 (in Chinese).Google Scholar
Ye, J (2000) 100 Questions about Culture of Tropical Fish. Fuzhou: Fujian Science and Technology Press (in Chinese).Google Scholar
Ye, YZ, Wu, QJ and Chen, RD (1989) Studies on cytology of crosses between grass carp and carp — asynchronization between nucleus and cytoplasm in distant hybridization of fishes. Acta Hydrobiologica Sinica 13: 234239 (in Chinese).Google Scholar
Yu, JH, Xia, DQ, Yang, H, He, YH and Wu, TT (2003) Morphology of the progenies of Oreochromis aurea (female)×Siniperca chuatsi (male). Journal of Fisheries of China 27: 431435 (in Chinese).Google Scholar
Zhang, JX, Liu, XF, Wang, ZX and Jin, GQ (1984) A comparative study on the karyotypes among the hybrid fish (Sinilabeo decorus tungting ♂×Cirrhinus molitorellafe ♀) and its parental fishes. Acta Hydrobiologica Sinica 8: 313322 (in Chinese).Google Scholar