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Specific identification of some female Empoascini (Hemiptera: Cicadellidae), using morphological characters of the ovipositor and isozyme and mtCOI sequence analyses

Published online by Cambridge University Press:  02 April 2012

Stefano Demichelis ,
Aulo Manino ,
Chiara Sartor ,
Dina Cifuentes  and
Augusto Patetta
Stefano Demichelis*
Affiliation:
Dipartimento di Valorizzazione e Protezione delle Risorse Agro-Forestali, Entomologia e Zoologia applicate all'Ambiente “Carlo Vidano,” Università di Torino, via Leonardo da Vinci 44, I-10095 Grugliasco, Torino, Italy
Aulo Manino
Affiliation:
Dipartimento di Valorizzazione e Protezione delle Risorse Agro-Forestali, Entomologia e Zoologia applicate all'Ambiente “Carlo Vidano,” Università di Torino, via Leonardo da Vinci 44, I-10095 Grugliasco, Torino, Italy
Chiara Sartor
Affiliation:
Dipartimento di Valorizzazione e Protezione delle Risorse Agro-Forestali, Entomologia e Zoologia applicate all'Ambiente “Carlo Vidano,” Università di Torino, via Leonardo da Vinci 44, I-10095 Grugliasco, Torino, Italy
Dina Cifuentes
Affiliation:
Departamento Producción Vegetal, Escuela Técnica Superior de Ingenieros Agrónomos, Universidad Politécnica de Cartagena. Paseo Alfonso XIII, 48, 30203 Cartagena, Spain
Augusto Patetta
Affiliation:
Dipartimento di Valorizzazione e Protezione delle Risorse Agro-Forestali, Entomologia e Zoologia applicate all'Ambiente “Carlo Vidano,” Università di Torino, via Leonardo da Vinci 44, I-10095 Grugliasco, Torino, Italy
*
1 Corresponding author (e-mail: stefano.demichelis@unito.it).
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Abstract

We identified females of six species of leafhoppers (tribe Empoascini), Asymmetrasca decedens (Paoli), Empoasca affinis Nast, E. decipiens Paoli, E. pteridis (Dahlbom), E. vitis Göthe, and Jacobiasca lybica (Bergevin and Zanon), using morphological characters of the ovipositor and isozyme and mitochondrial COI sequence analyses. Useful traits of the ovipositor are tooth morphology, length of the toothed area, form of sculpturing, and number and position of sensilla on the first and second pair of valves. Among the set of enzyme systems assayed, four putative loci, sod, hk-2, est-3, and est-2, together provided reliable diagnostic characters for single species, pairs of species, and groups of species. Analysis of mtCOI sequences revealed differences between the six species of Empoascini, and the neighbour-joining tree separated them into three distinct groups. Phenetic trees elaborated using Nei’s index and the Kimura two-parameter model clustered the six Empoascini species in similar groups.

Résumé

Nous avons identifié des femelles de six espèces de la tribu Empoascini, Asymmetrasca decedens (Paoli), Empoasca affinis Nast, E. decipiens Paoli, E. pteridis (Dahlbom), E. vitis Göthe et Jacobiasca lybica (Bergevin and Zanon) avec les caractères morphologiques de la tarière, et avec les études des isoenzymes et du gène mtCOI. Pour l'identification, des caractères de la lame antérieure et postérieure de la tarière ont été utilisés, particulièrement la forme des dents, la longueur de l'espace occupé par les dents, la forme de la surface occupée par des sculptures, le nombre et la position des sensilles. Les quatre loci qui peuvent être utilisés ensemble comme caractères diagnostiques sont sod, hk-2, est-3, et est-2. L’analyse des séquençages du gène mtCOI a montré des différences entre le six espèces de la tribu Empoascini, et le dendrogramme neighbour-joining a séparé trois groupes d'espèces. Les dendrogrammes basés sur les distances génétiques de Nei et la matrice de Kimura regroupent les six espèces d'Empoascini de la même façon.

Type
Articles
Information
The Canadian Entomologist , Volume 142 , Issue 6 , December 2010 , pp. 513 - 531
Copyright
Copyright © Entomological Society of Canada 2010

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