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Specific identification of some female Empoascini (Hemiptera: Cicadellidae), using morphological characters of the ovipositor and isozyme and mtCOI sequence analyses

  • Stefano Demichelis (a1), Aulo Manino (a1), Chiara Sartor (a1), Dina Cifuentes (a2) and Augusto Patetta (a1)...

Abstract

We identified females of six species of leafhoppers (tribe Empoascini), Asymmetrasca decedens (Paoli), Empoasca affinis Nast, E. decipiens Paoli, E. pteridis (Dahlbom), E. vitis Göthe, and Jacobiasca lybica (Bergevin and Zanon), using morphological characters of the ovipositor and isozyme and mitochondrial COI sequence analyses. Useful traits of the ovipositor are tooth morphology, length of the toothed area, form of sculpturing, and number and position of sensilla on the first and second pair of valves. Among the set of enzyme systems assayed, four putative loci, sod, hk-2, est-3, and est-2, together provided reliable diagnostic characters for single species, pairs of species, and groups of species. Analysis of mtCOI sequences revealed differences between the six species of Empoascini, and the neighbour-joining tree separated them into three distinct groups. Phenetic trees elaborated using Nei’s index and the Kimura two-parameter model clustered the six Empoascini species in similar groups.

Nous avons identifié des femelles de six espèces de la tribu Empoascini, Asymmetrasca decedens (Paoli), Empoasca affinis Nast, E. decipiens Paoli, E. pteridis (Dahlbom), E. vitis Göthe et Jacobiasca lybica (Bergevin and Zanon) avec les caractères morphologiques de la tarière, et avec les études des isoenzymes et du gène mtCOI. Pour l'identification, des caractères de la lame antérieure et postérieure de la tarière ont été utilisés, particulièrement la forme des dents, la longueur de l'espace occupé par les dents, la forme de la surface occupée par des sculptures, le nombre et la position des sensilles. Les quatre loci qui peuvent être utilisés ensemble comme caractères diagnostiques sont sod, hk-2, est-3, et est-2. L’analyse des séquençages du gène mtCOI a montré des différences entre le six espèces de la tribu Empoascini, et le dendrogramme neighbour-joining a séparé trois groupes d'espèces. Les dendrogrammes basés sur les distances génétiques de Nei et la matrice de Kimura regroupent les six espèces d'Empoascini de la même façon.

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Corresponding author

1 Corresponding author (e-mail: stefano.demichelis@unito.it).

References

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Specific identification of some female Empoascini (Hemiptera: Cicadellidae), using morphological characters of the ovipositor and isozyme and mtCOI sequence analyses

  • Stefano Demichelis (a1), Aulo Manino (a1), Chiara Sartor (a1), Dina Cifuentes (a2) and Augusto Patetta (a1)...

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