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NEUTRAL LIPID CONTENT AND FATTY ACID COMPOSITION OF 1,2-DIACYLGLYCEROLS IN THE HEMOLYMPH OF THE MALE GYPSY MOTH, LYMANTRIA DISPAR L. (LEPIDOPTERA: LYMANTRIIDAE)

Published online by Cambridge University Press:  31 May 2012

J. Marshall Clark ,
J.R. Marion ,
L.J. Scarano ,
T.L. Potter ,
P.F. Gosselin ,
J.A. Argentine  and
J.S. Elkinton
J. Marshall Clark
Affiliation:
Department of Entomology, Femald Hall, University of Massachusetts, Amherst, Massachusetts, USA 01003
J.R. Marion
Affiliation:
Department of Entomology, Femald Hall, University of Massachusetts, Amherst, Massachusetts, USA 01003
L.J. Scarano
Affiliation:
Department of Entomology, Femald Hall, University of Massachusetts, Amherst, Massachusetts, USA 01003
T.L. Potter
Affiliation:
Department of Entomology, Femald Hall, University of Massachusetts, Amherst, Massachusetts, USA 01003
P.F. Gosselin
Affiliation:
Department of Entomology, Femald Hall, University of Massachusetts, Amherst, Massachusetts, USA 01003
J.A. Argentine
Affiliation:
Department of Entomology, Femald Hall, University of Massachusetts, Amherst, Massachusetts, USA 01003
J.S. Elkinton
Affiliation:
Department of Entomology, Femald Hall, University of Massachusetts, Amherst, Massachusetts, USA 01003
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Abstract

Qualitative and quantitative changes in the neutral lipid content of hemolymph of gypsy moths, Lymantria dispar L., were assayed in larval, pupal, and adult stages. The major neutral lipid constituents of the hemolymph were 1,2-diacylglycerols and ranged in nominal concentration from 1.6–3.4 (larval), 3.1–4.9(pupal),toamaximumof 19.3 μg lipid per microlitre hemolymph in the adult male. When detected, triacylglycerols and monoacylglycerols never exceed diacylglycerol concentrations. The fatty acid composition of 1,2-diacylglycerols from adult male moths (0–12 h after emergence) was determined as fatty acid methyl esters using gas chromatography/mass spectrometry analysis. Nine fatty acid structures have been assigned. Of these, five are saturated, unbranched, aliphatic fatty acids (C14:0 – C18:0) which comprise 80.5% of the total fatty acid abundance. The remaining four fatty acids consist of two saturated, methyl-branched, aliphatic compounds, a mono-unsaturated aliphatic acid, and a tri-unsaturated, tricyclic, diterpenoid acid.

Résumé

Les modifications qualitatives et quantitatives des stades de larves, pupes et adultes de la teneur en lipides gras neutres de l’hémolymphe de la spongieuse, Lymantria dispar L., ont été étudiées. Les constituants principaux en acides gras neutres de l’hémolymphe ont été les 1,2-diacylglycérols qui ont varié en concentration entre 1,6 à 3,4 μg de lipide par microlitre d’hémolymphe (larves), 3,1 à 4,9 (pupes), jusqu’à une concentration maximum de 19,3 (mâles adultes). Quand discernés, les glycérols triacyliques et le glycérol monoacylique n’ont jamais dépassé la concentration du glycérol diacylique. La composition en acides gras des 1,2-diacylglycérols des mâles adultes (0 à 12 h après l’éclosion) a été déterminée par analyse de chromatographie de gaz/spectrométrie de masse comme des esters méthyliques des acides gras. Neuf structures d’acides gras ont été fixées. De ceux-ci, cinq se trouvent des acides gras aliphatiques saturés, sans branche (C14 : 0 à C18 : 0), qui font 80,5% de l’abondance globale des acides gras. Les quatre acides gras qui restent se composent de deux composés aliphatiques saturés de branches méthyliques, un acide aliphatique non-saturé d’une branche et un acide diterpénoïde tricyclique triplement non-saturé.

Type
Research Article
Information
The Canadian Entomologist , Volume 122 , Issue 6 , December 1990 , pp. 1101 - 1109
Copyright
Copyright © Entomological Society of Canada 1990

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