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Molecular cloning, characterization, and expression analysis of a novel sema-2a homologue in Polyrhachis vicina (Hymenoptera: Formicidae)

Published online by Cambridge University Press:  02 April 2012

Jing Luo ,
Geng-Si Xi ,
Shu-Min Lü ,
Ke Li  and
Qing Li
Jing Luo
Affiliation:
College of Life Sciences, Shaanxi Normal University, Xi'an, 710062, People's Republic of China
Geng-Si Xi*
Affiliation:
College of Life Sciences, Shaanxi Normal University, Xi'an, 710062, People's Republic of China
Shu-Min Lü
Affiliation:
College of Plant Protection, Northwest A&F University, Yangling, 712100, People's Republic of China
Ke Li
Affiliation:
College of Life Sciences, Shaanxi Normal University, Xi'an, 710062 and Linfen, 041004, People's Republic of China
Qing Li
Affiliation:
College of Life Sciences, Shaanxi Normal University, Xi'an, 710062, People's Republic of China
*
1 Corresponding author (e-mail: xigengsi@snnu.edu.cn).
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Abstract

The semaphorin gene family plays important roles in axonal guidance in vertebrates and invertebrates. Semaphorin 2a, a member of the semaphorin family, belongs to class 2, which is found only in invertebrates. In our study, semaphorin 2a was cloned from the ant Polyrhachis vicina Roger. The full length of P. vicina semaphorin 2a (Pv-sema-2a) is 2763 base pairs (bp) and it contains a 5′-untranslated region (UTR) 92 bp long and a 3′-UTR 521 bp long. The open reading frame of Pv-sema-2a encodes a 716-amino-acid protein with a predicted molecular mass of 81.1 kilodaltons. Real-time quantitative reverse-transcription – polymerase chain reaction indicated that Pv-sema-2a mRNA is differentially expressed during P. vicina development, in the whole bodies as well as the heads of different castes. The high mRNA levels in embryos and pupae suggest that Pv-sema-2a plays an important role in ant development.

Résumé

La famille des gènes de la sémaphorine jouent des rôles importants dans le guidage des axones chez les vertébrés et les invertébrés. La sémaphorine 2a, un membre de la famille des sémaphorines, appartient à la classe 2 qui se retrouve seulement chez les invertébrés. Nous avons, dans notre étude, cloné la sémaphorine 2 chez la fourmi Polyrhachis vicina Roger. La longueur totale de la sémaphorine 2a (Pv-sema-2a) de P. vicina est de 2 763 paires de bases (bp) et contient une région 5′ non traduite (5′-UTR) de 92 bp et une autre 3′-UTR de 521 bp. Le cadre ouvert de lecture (ORF) de Pv-sema-2a code une protéine de 716 acides aminés avec une masse moléculaire prédite de 81,1 kilodaltons. La RT-PCR quantitative en temps réel indique que l'ARNm Pv-sem2a est exprimé différemment au cours du développement de P. vicina, tant dans les corps entiers que dans les têtes des différentes castes. Les concentrations élevées de ARNm chez les embryons et les nymphes indiquent le rôle important de Pv-sema-2a dans le développement des fourmis.

[Traduit par la Rédaction]

Type
Articles
Information
The Canadian Entomologist , Volume 142 , Issue 1 , February 2010 , pp. 1 - 13
Copyright
Copyright © Entomological Society of Canada 2010

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