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A METHOD FOR SEPARATING AND SURFACE STERILIZING THE EGGS OF THE SPRUCE BUDWORM, CHORISTONEURA FUMIFERANA (LEPIDOPTERA: TORTRICIDAE)1

Published online by Cambridge University Press:  31 May 2012

Arthur Retnakaran  and
John French
Arthur Retnakaran
Affiliation:
Insect Pathology Research Institute, Canadian Forestry Service, Sault Ste. Marie, Ontario
John French
Affiliation:
Insect Pathology Research Institute, Canadian Forestry Service, Sault Ste. Marie, Ontario
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Abstract

A simple method for separating and surface sterilizing spruce budworm eggs is described. The egg masses are incubated with constant agitation in a 1% solution of NaOH for 10 minutes at 35 °C. The separated eggs are rinsed serially in distilled water, 1% bovine albumen solution, and 70% ethanol, and placed on a moist, sterile filter paper in a petri dish. When surface sterilized eggs are not required the 70% ethanol wash can be omitted.

Type
Articles
Information
The Canadian Entomologist , Volume 103 , Issue 5 , May 1971 , pp. 712 - 716
Copyright
Copyright © Entomological Society of Canada 1971

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References

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