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A METHOD FOR MASS PRODUCING SPORES OF THE MICROSPORIDIAN NOSEMA FUMIFERANAE IN ITS HOST, THE SPRUCE BUDWORM, CHORISTONEURA FUMIFERANA (LEPIDOPTERA: TORTRICIDAE)1

Published online by Cambridge University Press:  31 May 2012

G. G. Wilson
G. G. Wilson
Affiliation:
Insect Pathology Research Institute, Canadian Forestry Service, Sault Ste. Marie, Ontario
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Abstract

The mass production of Nosema fumiferanae spores using second, third, and fourth instar larvae of Choristoneura fumiferana is described. In general, second instar larvae inoculated with 2 × 105 spores per millilitre of suspension resulted in a maximum spore yield of 1.3 × 108 spores per larva. A spore concentration of 2 × 107 reduced larval weights and increased larval mortality.

Résumé

L’auteur décrit la production en masses des spores de Nosema fumiferanae, en utilisant les larves de 2e, 3e et 4e stades appartenant à Choristoneura fumiferana. En général, les larves de 2e stade inoculées avec 2 × 105 spores par ml de suspension produisirent un rendement maximal de 1.3 × 108 spores par larve. Des spores en concentration de 2 × 107 réduisirent le poids des larves et augmentèrent leur mortalité.

Type
Articles
Information
The Canadian Entomologist , Volume 108 , Issue 4 , April 1976 , pp. 383 - 386
Copyright
Copyright © Entomological Society of Canada 1976

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References

Hostounský, Z. and Weiser, J.. 1972. Production of spores of Nosema plodiae Kellen et Lindegren in Mamestra brassicae L. after different infective dosage, I. Vest. Cs. spol. zool. 36: 97–100.Google Scholar
McLaughlin, R. E. and Bell, M. R.. 1970. Mass production in vivo of two protozoan pathogens, Mattesia grandis and Glugea gasti, of the boll weevil, Anthonomus grandis J. invert. Path. 16: 84–88.Google Scholar
McMorran, A. 1965. A synthetic diet for the spruce budworm, Choristoneura fumiferana (Clem.) (Lepidoptera: Tortricidae). Can. Ent. 97: 58–62.Google Scholar
Raun, E. S., York, G. T., and Brooks, D. L.. 1960. Determination of Perezia pyraustae infection rates in larvae of the European corn borer. J. Insect Path. 2: 254–258.Google Scholar
Wilson, G. G. 1974. Effects of larval age at inoculation, and dosage of microsporidian (Nosema fumiferanae) spores, on mortality of spruce budworm (Choristoneura fumiferana). Can. J. Zool. 52: 993–996.Google Scholar