Skip to main content Accessibility help

Effects of four varieties of Lathyrus sativus (Fabaceae) seeds on the bionomics of Callosobruchus maculatus (Coleoptera: Chrysomelidae)

  • Poulami Adhikary (a1), Ujjwal Malik (a1) and Anandamay Barik (a1)


The effect of four varieties of Lathyrus sativus Linnaeus (Fabaceae) seeds (Bio L 212 Ratan, Nirmal B-1, WBK-14-7, and WBK-13-1) on the bionomics of Callosobruchus maculatus (Fabricius) (Coleoptera: Chrysomelidae: Bruchinae) was studied under laboratory conditions. Total larval developmental time was longer on WBK-13-1 than Bio L 212 Ratan. Adult emergence was highest on Bio L 212 Ratan followed by Nirmal B-1, WBK-14-7, and WBK-13-1. The development and fecundity were related with nutrient and antinutritional factors of khesari seeds. Total carbohydrates and proteins were highest in Bio L 212 Ratan and Nirmal B-1 while lipids and nitrogen were highest in Bio L 212 Ratan and least in WBK-13-1. Amino acids were higher in Nirmal B-1 and WBK-13-1. Phenols were greatest in Nirmal B-1 and least in Bio L 212 Ratan and WBK-14-7. β-ODAP was higher in Bio L 212 Ratan and Nirmal B-1. The lower levels of carbohydrates, proteins, lipids, nitrogen, water content, and higher trypsin inhibitor activity of WBK-14-7 and WBK-13-1 may explain the higher developmental time and lower fecundity of C. maculatus on these varieties. These results suggest that infestations of C. maculatus may be easier to manage on WBK-14-7 and WBK-13-1 than on the other varieties of khesari seeds.


Corresponding author

1 Corresponding author (e-mail:


Hide All

Subject editor: Gilles Boiteau



Hide All
Adhikary, P. and Barik, A. 2012. Effect of temperature on biology of Callosobruchus maculatus (F.). Indian Journal of Entomology, 74: 261266.
Appleby, J.H. and Credland, P.F. 2001. Bionomics and polymorphism in Callosobruchus subinnotatus . Bulletin of Entomological Research, 91: 235244.
Arnold, S.E.J., Stevenson, P.C., and Belmain, S.R. 2012. Odour-mediated orientation of beetles is influenced by age, sex and morph. Public Library of Science One, 7: e49071. doi:10.1371/journal.pone.0049071.
Bieri, J. and Kawecki, T.J. 2003. Genetic architecture of differences between populations of cowpea weevil (Callosobruchus maculatus) evolved in the same environment. Evolution, 57: 274287.
Bray, H.G. and Thorpe, W.V. 1954. Analysis of phenolic compounds of interest in metabolism. Methods of Biochemical Analysis, 1: 2752.
Briggs, C.J., Parreno, N., and Campbell, C.G. 1983. Phytochemical assessment of Lathyrus species for the neurotoxic agent, β-N-oxalyl-L-α, β-diaminopropionic acid. Planta Medica, 47: 188190.
Cope, J.M. and Fox, C.W. 2003. Oviposition decisions in the seed beetle, Callosobruchus maculatus (Coleoptera: Bruchidae): effects of seed size on superparasitism. Journal of Stored Products Research, 39: 355365.
Credland, P.F. 1987. Effects of host change on the fecundity and development of an unusual strain of Callosobruchus maculatus (F.) (Coleoptera: Bruchidae). Journal of Stored Products Research, 23: 9198.
Credland, P.F., Dick, K.M., and Wright, A.W. 1986. Relationships between larval density, adult size and egg production in the cowpea seed beetle Callosobruchus maculatus . Ecological Entomology, 11: 4150.
Credland, P.F. and Wright, W.A. 1989. Factors affecting female fecundity in the cowpea seed beetle, Callosobruchus maculatus (Coleoptera: Bruchidae). Journal of Stored Products Research, 25: 125136.
Denlinger, D.L. 1986. Dormancy in tropical insects. Annual Review of Entomology, 31: 239264.
Downer, R.G.H. and Matthews, J.R. 1976. Patterns of lipid distribution and utilization in insects. American Zoologist, 16: 733745.
Dubios, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A., and Smith, F. 1956. Colorimetric method for determination of sugars and related substances. Analytical Chemistry, 28: 350356.
Folch, J., Lees, M., and Solane-Stanley, G.H. 1957. A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry, 226: 497509.
Fox, C.W. 1993. The influence of maternal age and mating frequency on egg size and offspring performance in Callosobruchus maculatus (Coleoptera: Bruchidae). Oecologia, 96: 139146.
Fox, C.W., Bush, M.L., and Messina, F.J. 2010. Biotypes of the seed beetle Callosobruchus maculatus have differing effects on the germination and growth of their legume hosts. Agricultural and Forest Entomology, 12: 353362.
Gaur, V. and Maloo, S.R. 2011. Genetic divergence in grass pea (Lathyrus sativus). Indian Journal of Agricultural Science, 80: 172173.
Giga, D.P. and Smith, R.H. 1983. Comparative life history studies on four Callosobruchus species infesting cowpeas with special reference to Callosobruchus rhodesianus (Coleoptera: Bruchidae). Journal of Stored Products Research, 19: 189198.
Girma, D. and Korbu, L. 2012. Genetic improvement of grass pea (Lathyrus sativus) in Ethiopoia: an unfulfilled promise. Plant Breeding, 131: 231236.
Hagstrum, D.W. and Milliken, G.A. 1988. Quantitative analysis of temperature, moisture, and diet factors affecting insect development. Annals of the Entomological Society of America, 81: 539546.
Harborne, J.B. 2003. Introduction to ecological biochemistry. Academic Press, London, United Kingdom.
Huignard, J., Leroi, B., Alzouma, I., and Germain, J.F. 1985. Oviposition and development of Bruchidius atrolineatus (Pic) and Callosobruchus maculatus (F.) in Vigna unguiculata (Walp) in cultures in Niger. Insect Science and its Application, 6: 691699.
Humphries, E.C. 1956. Nitrates. In Modern methods of plant analysis. Edited by K. Peach and M.V. Tracey. Springer Verlag, Berlin, Germany. Pp. 481483.
Kakade, M.L., Rackis, J.J., McGhee, J.E., and Puski, G. 1974. Determination of trypsin inhibitor activity of soy products; a collaborative analysis of an improved procedure. Cereal Chemistry, 51: 376382.
Lenga, A., Thibeaudeau, C., and Huignard, J. 1991. Influence of thermoperiod and photoperiod on reproductive diapauses in Bruchidius atrolineatus (Pic) (Coleoptera, Bruchidae). Physiological Entomology, 16: 295303.
Liener, I.E. and Kakade, M.L. 1980. Protease inhibitors. In Toxic constituents of plant foodstuffs. Edited by I.E. Liener. Academic Press, New York, New York, United States of America. Pp. 771.
Lowry, O.H., Rose Brough, N.G., Farr, A.L., and Randall, R.G. 1951. Protein measurements with folin phenol reagent. Journal of Biological Chemistry, 183: 265275.
Mattson, W.J. and Scriber, J.M. 1987. Nutritional ecology of insect folivores of woody plants: nitrogen, water, fiber and mineral conditions. In Nutritional ecology of insects, mites, spikes and related invertebrates. Edited by F.J.R. Slansky and J.G. Rodriguez. John Wiley and Sons, New York, New York, United States of America. Pp. 105146.
Messina, F.J. 1987. Genetic contribution to the dispersal polymorphism of the cowpea weevil (Coleoptera: Bruchidae). Annals of the Entomological Society of America, 80: 1216.
Messina, F.J. 1991. Life-history variation in a seed beetle: adult egg-laying vs. larval competitive ability. Oecologia, 85: 447455.
Messina, F.J. and Renwick, J.A.A. 1985a. Dispersal polymorphism of Callosobruchus maculatus (Coleoptera: Bruchidae): variation among populations in response to crowding. Annals of the Entomological Society of America, 78: 201206.
Messina, F.J. and Renwick, J.A.A. 1985b. Resistance of Callosobruchus maculatus (Coleoptera: Bruchidae) in selected cowpea lines. Environmental Entomology, 14: 868 872.
Messina, F.J. and Slade, A.F. 1999. Expression of a life-history trade-off in a seed beetle depends on environmental context. Physiological Entomology, 24: 358363.
Moore, R.A. and Stein, W.H. 1948. Photometric ninhydrin method for use in the chromatography of amino acids. Journal of Biological Chemistry, 176: 367388.
Nation, J.L. 2001. Insect physiology and biochemistry. CRC Press, Boca Raton, Florida, United States of America.
Oppert, B., Morgan, T.D., Culbertson, C., and Kramer, K.J. 1993. Dietary mixtures of cysteine and serine proteinase inhibitors exhibit synergistic toxicity toward the red flour beetle, Tribolium castaneum . Comparative Biochemistry and Physiology Part C: Comparative Pharmacology, 105: 379385.
Radha, R. and Susheela, P. 2014. Studies on the life history and ovipositional preference of Callosobruchus maculatus reared on different pulses. Research Journal of Animal, Veterinary and Fishery Sciences, 2: 15.
Rao, S.L.N. 1978. A sensitive and specific colorimetric method for the determination of α, β-diaminopropionic acid and the Lathyrus sativus neurotoxin. Analytical Biochemistry, 86: 386395.
Rao, S.L.N. 2011. A look at the brighter facets of β-N-oxalyl-L-α, β-diaminopropionic acid, homoarginine and the grass pea. Food and Chemical Toxicology, 49: 620622.
Roy, N. and Barik, A. 2013. Influence of four host plants on feeding, growth and reproduction of Diacrisia casignetum (Lepidoptera: Arctiidae). Entomological Science, 16: 112118.
Sano, I. 1967. Density effect and environmental temperature as the factors producing the active form of Callosobruchus maculatus (F.) (Coleoptera, Bruchidae). Journal of Stored Products Research, 2: 187195.
Sano-Fujii, I. 1984. Effect of bean water content on the production of the active form of Callosobruchus maculatus (F.) (Coleoptera: Bruchidae). Journal of Stored Products Research, 20: 153161.
Sarkar, N., Mukherjee, A., and Barik, A. 2015. Attraction of Epilachna dodecastigma (Coleoptera: Coccinellidae) to Momordica charantia (Cucurbitaceae) leaf volatiles. The Canadian Entomologist, 147: 169180. doi:10.4039/tce.2014.37.
Scriber, J.M. and Feeny, P. 1979. Growth of herbivorous caterpillars in relation to feeding specialization and to the growth form of their food plants. Ecology, 60: 829850.
Singh, S.S. and Rao, S.L.N. 2013. Lessons from neurolathyrism: a disease of the past and the future of Lathyrus sativus (Khesari dal). Indian Journal of Medical Research, 138: 3237.
Slansky, F. and Scriber, J.M. 1985. Food consumption and utilization. In Comprehensive insect physiology biochemistry and pharmacology. Edited by G.A. Kerdut and L.I. Gilbert. Pergamon Press, Oxford, United Kingdom. Pp. 87113.
Southgate, B.J. 1979. Biology of the Bruchidae. Annual Review of Entomology, 24: 449473.
Thanthianga, C. and Mitchell, R. 1990. The fecundity and oviposition behaviour of a south Indian strain of Callosobruchus maculatus . Entomologia Experimentalis et Applicata, 57: 133142.
Timms, R. 1998. Size-independent effects of larval host on adult fitness in Callosobruchus maculatus . Ecological Entomology, 23: 480483.
Tuda, M., Chou, L.-Y., Niyomdham, C., Buranapanichpan, S., and Tateishi, Y. 2005. Ecological factors associated with pest status in Callosobruchus (Coleoptera: Bruchidae): high host specificity of non-pests to Cajaninae (Fabaceae). Journal of Stored Products Research, 41: 3145.
Tuda, M., Kagoshima, K., Toquenaga, Y., and Arnqvist, G. 2014. Global genetic differentiation in a cosmopolitan pest of stored beans: effects of geography, host-plant usage and anthropogenic factors. Public Library of Science One, 9: e106268. doi:10.1371/journal.pone.0106268.
Tuda, M., Ronn, J., Buranapanichpan, S., Wasano, N., and Arnqvist, G. 2006. Evolutionary diversification of the bean beetle genus Callosobruchus (Coleoptera: Bruchidae): traits associated with stored-product pest status. Molecular Ecology, 15: 35413551.
Utida, S. 1972. Density dependent polymorphism in the adult of Callosobruchus maculatus . Journal of Stored Products Research, 8: 111125.
Utida, S. 1981. Polymorphism and phase dimorphism in Callosobruchus maculatus . In The ecology of bruchids attacking legumes. Edited by V. Labeyrie. W. Junk Publishers, The Hague, The Netherlands. Pp. 143147.
Wang, X.F., Warkentin, T.D., Briggs, C.J., Oomah, B.D., Campbell, C.G., and Woods, S. 1998. Trypsin inhibitor activity in field pea (Pisum sativum L.) and grass pea (Lathyrus sativus L.). Journal of Agricultural Food Chemistry, 46: 26202623.
Wasserman, S.S. 1988. Partial paternal inheritance of realized fecundity in a bruchid beetle, Callosobruchus maculatus . Behavioural Genetics, 18: 193200.
Zannou, E.T., Glitho, I.A., Huignard, J., and Monge, J.P. 2003. Life history of flight morph females of Callosobruchus maculatus F.: evidence of a reproductive diapause. Journal of Insect Physiology, 49: 575582.
Zar, J.H. 1999. Biostatistical analysis. Prentice Hall, Upper Saddle River, New Jersey, United States of America.

Effects of four varieties of Lathyrus sativus (Fabaceae) seeds on the bionomics of Callosobruchus maculatus (Coleoptera: Chrysomelidae)

  • Poulami Adhikary (a1), Ujjwal Malik (a1) and Anandamay Barik (a1)


Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed