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LABORATORY BIOASSAYS OF MIXTURES OF BACILLUS THURINGIENSIS AND CHITINASE1

Published online by Cambridge University Press:  31 May 2012

Paul G. Fast
Paul G. Fast
Affiliation:
Insect Pathology Research Institute, Canadian Forestry Service, Sault Ste. Marie, Ontario P6A 5M7
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Abstract

The addition of chitinase at the rate of 1000–4000 nephelometric units/U.S. gallon to Bacillus thuringiensis insecticide for use against spruce budworm has been advocated.

Bioassays of V instar spruce budworm larvae against seven levels of Thuricide® at four levels of chitinase corresponding to 0, 22, 220, and 2200 nephelometric units/U.S. gallon were conducted by placing larvae on balsam fir foliage (Abies balsamea) dipped in appropriate dilutions of Thuricide® with chitinase. Mortality was assessed 6 days later. An additional replicated assay at a relatively massive dose of chitinase, 33,000 nephelometric units/U.S. gallon, was compared simultaneously with a 0 chitinase control. The data were homogeneous and linear by statistical standards. No effect of chitinase on LC50 or slope of the regression line was observed.

Type
Articles
Information
The Canadian Entomologist , Volume 110 , Issue 2 , 01 February 1978 , pp. 201 - 203
Copyright
Copyright © Entomological Society of Canada 1978

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