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BATCH SURFACE STERILIZATION OF CHIRONOMID EGGS

Published online by Cambridge University Press:  31 May 2012

B. E. Townsend  and
R. D. Hamilton
B. E. Townsend
Affiliation:
Freshwater Institute, Environment Canada, Winnipeg, Manitoba
R. D. Hamilton
Affiliation:
Freshwater Institute, Environment Canada, Winnipeg, Manitoba
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Abstract

A simple apparatus for the surface sterilization of a large number of chironomid eggs is described. The apparatus allows as many as 4000 eggs to be processed in 20 min without the inconvenience of manual transfer and washing procedures. Hatching percentages of untreated and treated eggs were 97.2% and 96.4% respectively. The hatching percentage of eggs produced by females that had developed from treated eggs was 96%. Comparisons between percentage emergence, sex ratios, adult viability, and fecundity of untreated and treated animals indicated that surface sterilization had little or no effect on chironomid development.

Type
Articles
Information
The Canadian Entomologist , Volume 108 , Issue 6 , June 1976 , pp. 669 - 671
Copyright
Copyright © Entomological Society of Canada 1976

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