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Polyphasic approach applying artificial neural networks, molecular analysis and postabdomen morphology to West Palaearctic Tachina spp. (Diptera, Tachinidae)

Published online by Cambridge University Press:  20 October 2010

N. Muráriková ,
J. Vaňhara ,
A. Tóthová  and
J. Havel
N. Muráriková
Affiliation:
Masaryk University, Faculty of Science, Kotlářská 2, CZ-611 37 Brno, Czech Republic
J. Vaňhara*
Affiliation:
Masaryk University, Faculty of Science, Kotlářská 2, CZ-611 37 Brno, Czech Republic
A. Tóthová
Affiliation:
Masaryk University, Faculty of Science, Kotlářská 2, CZ-611 37 Brno, Czech Republic
J. Havel
Affiliation:
Masaryk University, Faculty of Science, Kotlářská 2, CZ-611 37 Brno, Czech Republic
*
*Author for correspondence Fax: +420 532 146 213 E-mail: vanhara@sci.muni.cz
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Abstract

Artificial neural networks (ANN) methodology, molecular analyses and comparative morphology of the male postabdomen were used successfully in parallel for species identification and resolution of some taxonomic problems concerning West Palaearctic species of the genus Tachina Meigen, 1803. Supervised feed-forward ANN with back-propagation of errors was applied on morphometric and qualitative characters to solve known taxonomic discrepancies. Background molecular analyses based on mitochondrial markers CO I, Cyt b, 12S and 16S rDNA and study of male postabdominal structures were published separately. All three approaches resolved taxonomic doubts with identical results in the following five cases: case 1, the four presently recognized subgenera of the genus Tachina were confirmed and the description of a new subgenus was recommended; case 2, the validity of a new boreo-alpine species (sp.n.) was confirmed; case 3, the previously supposed presence of T. casta (Rondani, 1859) in central Europe was not supported; case 4, West Palaearctic T. nupta (Rondani, 1859) was contrasted with East Palaearctic specimens from Japan, which seem to represent a valid species not conspecific with central European specimens; T. nupta needs detailed further study; case 5, T. nigrohirta (Stein, 1924) resurrected recently from synonymy with T. ursina Meigen, 1824 was confirmed as a valid species. This parallel application of three alternative methods has enabled the principle of ‘polyphasic taxonomy’ to be tested and verified using these separate results. For the first time, the value of using the ANN approach in taxonomy was justified by two non-mathematical methods (molecular and morphological).

Keywords

ANN - artificial neural networksmolecular markers CO ICyt b12S16S rDNAmale postabdomenspecies identificationpolyphasic taxonomyDipteraTachinidaeTachinaparasitoids
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 101 , Issue 2 , April 2011 , pp. 165 - 175
Copyright
Copyright © Cambridge University Press 2010

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