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Observations on the larvae and pupae of Pieris brassicae (L.) in a laboratory culture

Published online by Cambridge University Press:  10 July 2009

W. A. L. David  and
B. O. C. Gardiner
W. A. L. David
Affiliation:
Agricultural Research Council Unit of Insect Physiology, Cambridge.
B. O. C. Gardiner
Affiliation:
Agricultural Research Council Unit of Insect Physiology, Cambridge.
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Extract

The work described in this paper forms the final part of an investigation into the biology and breeding of Pieris brassicae (L.) in captivity and concerns the larvae and the pupae.

The larvae of the Cambridge stock used in this investigation were found to pass through five instars in the course of their development at temperatures between 12·5 and 30°C. At the lower temperature, development was completed in 46·5 days and at the higher temperature in 11 days.

The average width of the head capsules in each instar was not affected by the temperature at which the larvae were reared, it showed little variation, and it never overlapped with that of the preceding or ensuing instar and, therefore, provides a certain way of determining the instar of any larva.

At 20°C., isolated larvae and larvae kept in crowded cultures completed their development in approximately the same time—19·6 and 18·8 days, respectively.

The average consumption of food during the whole larval period was determined in two experiments, in which it was found to be 1·42 and 1·29 g. of fresh leaves per g. of larva per day, respectively.

The duration of the pupal period ranged from 7·5 days at 30°C. to about 40 days at 12·5°C.

The adults showed a definite diel rhythm of emergence. When kept at a constant temperature, with a photoperiod from 6 a.m. to 10 p.m., nearly all the insects emerged during the dark period and that immediately following it—actually between the hours of 1 a.m. and 9 a.m. If the photoperiod is displaced 12 hours, the emergence is also displaced by the same amount, to correspond with the new dark period. If, instead of keeping the temperature constant, with the photoperiod 6 a.m. to 10 p.m., it is allowed to fluctuate, as it does naturally in June, the emergence is delayed and instead of occurring in darkness and the early hours of the morning as it does at a constant temperature, it takes place mainly during the morning and the afternoon. When insects, which have been reared at a constant temperature and a photoperiod from 6 a.m. to 10 p.m., are allowed to emerge at a constant temperature, in continuous light, there is very little evidence of a diel rhythm of eclosion but if the insects are kept in continuous darkness they show a definite rhythm of emergence. If the pupae are kept in constant light but the temperature is allowed to fluctuate, most of the adults emerge during the warmer period of the cycle.

Diapause in the pupa of P. brassicae is mainly determined by the photoperiod and the temperature during the larval stages. At 20°C., larvae reared in continuous darkness do not form diapause pupae; as the daily photoperiod increases, the percentage of diapause pupae formed also increases until, at a photoperiod of 12 hours, only diapause pupae are formed. Beyond this point the percentage of diapause pupae again declines until, with a photoperiod of about 18 hours, only non-diapause pupae are formed. At higher temperatures similar trends are observed but lower percentages of diapause pupae are formed at all photoperiods.

In P. brassicae there is no evidence that a short, sharply defined period of a day or two exists in the course of the life of the larvae during which the photoperiod operates to influence diapause.

Non-diapause pupae produced from larvae reared in continuous darkness and from larvae reared in long days (over 15 hours' light) appear to contain a growth-promoting hormone capable of causing the emergence of diapause pupae.

Type
Research Paper
Information
Bulletin of Entomological Research , Volume 53 , Issue 2 , July 1962 , pp. 417 - 436
Copyright
Copyright © Cambridge University Press 1962

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References

Beck, S. D. (1950). Nutrition of the European corn borer, Pyrausta nubilalis (Hbn.). II. Some effects of diet on larval growth characteristics.— Physiol. Zoöl. 23 pp. 353–361.CrossRefGoogle ScholarPubMed
Brett, W. J. (1955). Persistent diurnal rhythmicity in Drosophila emergence.— Ann. ent. Soc. Amer. 48 pp. 119–131.Google Scholar
Bünning, E. & Joerrens, G. (1960). Tagesperiodische antagonistiche Schwan-kungen der Blauviolett- und Gelbrot-Empfindlichkeit als Grundlage der photoperiodischen Diapause-Induktion bei Pieris brassicae.—Z. Naturf. 15b pp. 205–213.Google Scholar
David, W. A. L. (1957). Breeding Pieris brassicae L. and Apanteles glomeratus L. as experimental insects.—Z. PflKrankh. 64 pp. 572–577.Google Scholar
David, W. A. L. & Gardiner, B. O. C. (1952). Laboratory breeding of Pieris brassicae L. and Apanteles glomeratus L.—Proc. R. ent. Soc. Lond. (A) 27 pp. 54–56.Google Scholar
David, W. A. L. & Gardiner, B. O. C. (1961a). The mating behaviour of Pieris brassicae (L.) in a laboratory culture.—Bull. ent. Res. 52 pp. 263–280.CrossRefGoogle Scholar
David, W. A. L. & Gardiner, B. O. C. (1961b). Feeding behaviour of adults of Pieris brassicae (L.) in a laboratorv culture.—Bull, ent Res. 52 pp 741–762.Google Scholar
David, W. A. L. & Gardiner, B. O. C. (1962). Oviposition and the hatching of the eggs of Pieris brassicae (L.) in a laboratory culture.—Bull, ent Res. 53 pp. 91–109.CrossRefGoogle Scholar
Dickson, R. C. (1950). Factors governing the induction of diapause in the oriental fruit moth.—Ann. ent. Soc. Amer. 42 pp. 511–537.Google Scholar
Evans, A. C. (1938). Physiological relationships between insects and their host plants. I. The effect of the chemical composition of the plant on reproduction and production of winged forms in Brevicoryne brassicae L. (Aphididae).—.Ann. appl. Biol. 25 pp. 558–572.Google Scholar
Evans, A. C. (1939). The utilisation of food by certain Lepidopterous larvae.— Trans. R. ent. Soc. Land. 89 pp. 13–22.Google Scholar
Grison, P. & Silvestre De Sacy, R. (1957). L'élevage de Pieris brassicae L. pour les essais de traitements microbiologiques.—Ann. Épiphyt. 7 pp. 661–674.Google Scholar
Klein, H. Z. (1932a). Studien zur Ökologie und Epidemiologie der Kohlweisslinge. I. Der Einfluss der Temperatur und Luftfeuchtigkeit auf Entwicklung und Mortalität von Pieris brassicae L.—Z. angew. Ent. 19 395–448.CrossRefGoogle Scholar
Klein, H. Z. (1932b). Studien zur Oekologie und Epidemiologie der Kohlweisslinge. II. Zur Bionomie von Pieris brassicae, L. und deren Parasit Microgaster glomeratus L.—Z. wiss. InsektBiol. 26 pp. 192–199.Google Scholar
Long, D. B. (1953). Effects of population density on larvae of Lepidoptera.— Trans. R. ent. Soc. Lond. 104 pp. 543–586.Google Scholar
Palmén, E. (1955). Diel periodicity of pupal emergence in natural populations of some chironomids (Diptera).—Ann. zool. Soc. zool.-bot. fenn. Vanamo 17 no. 3, 30 pp.Google Scholar
Way, M. J. & Hopkins, B. A. (1950). The influence of photoperiod and temperature on the induction of diapause in Diataraxia oleracca L. (Lepidoptera).—J. exp. Biol. 27 pp. 365–376.Google Scholar
Way, M. J., Hopkins, B. & Smith, P. M. (1949). Photoperiodism and diapause in insects.—Nature, Lond. 164 p. 615.Google Scholar
Wigglesworth, V. B. (1934). The physiology of ecdysis in Rhodnius prolixus (Hemiptera). II. Factors controlling moulting and “ metamorphosis.”— Quart. J. micr. Sci. 77 pp. 191–222.Google Scholar
Wigglesworth, V. B. (1953). The principles of insect physiology.—5th edn., 546pp. London, Methuen; New York, Dutton.Google Scholar
Williams, C. M. (1946). Physiology of insect diapause: the rôle of the brain in the production and termination of pupal dormancy in the giant silkworm, Platysamia cecropia.—Biol. Bull., Wood's Hole 90 pp. 234–243.Google Scholar
Zaher, M. A. & Long, D. B. (1959). Some effects of larval population density on the biology of Pieris brassicae L. and Plusia gamma L.—Proc. R. ent. Soc. Lond. (A) 34 pp. 97–109.Google Scholar