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A latex agglutination test for the identification of blood-meals of Culicoides (Diptera: Ceratopogonidae)

  • J. Boorman (a1), P. S. Mellor (a1), P. F. L. Boreham (a2) and Rosemary S. Hewett (a2)

Abstract

A latex agglutination test to identify the sources of blood-meals of Culicoides is described. The method requires the minimum amounts of reagents and apparatus and would be suitable for rapid tests under field conditions. Blood-meals of Culicoides nubeculosus (Mg.) and C. variipennis (Coq.) from laboratory cultures, which had engorged on blood from several species of domestic animals, were differentiated without difficulty. In a series of ‘blind’ tests, the blood-meals of 49 C. variipennis from seven species of domestic animal were identified with 100% accuracy. The differentiation of blood-meals from closely related hosts, such as mouse and guineapig or ox and sheep, is entirely dependent upon the specificity of the antisera conjugated to the latex. Blood-meals could be identified reliably for about 48 h after ingestion at 20–25°C, but at higher temperatures the reaction became less reliable after 24 h. Detergents commonly used in collecting midges had no effect on the latex agglutination test, but 0–01% mertbiolate, often used as a preservative, caused auto-agglutination and cannot be used when this test is to be applied.

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Abu Salih, H. S.., Murant, A. F., Daft, M. J.. (1968). The use of antibody-sensitised latex particles to detect plant viruses. — J. gen. Virol. 3, 299302.
Bercks, R., Querfurth, G.. (1969). Weitere methodische Utersuchungen über den Latextest zum serologischen Nachweis pflanzenpathogener Viren.— Phytopath. Z. 65, 243256.
Boreham, P. F. L.. (1975). Some applications of bloodmeal identifications in relation to the epidemiology of vector-borne tropical diseases. — J. trop. Med. Hyg. 78, 8391.
Boreham, P. F. L. (in press). Sterilisation of arthropod bloodmeals prior to bloodmeal identification. — Mosquito News.
Boreham, P. F. L., Gill, G. S.. (1973). Serological identification of reptile feeds of Glossina. — Acta Trop. 30, 356365.
Braverman, Y.., Boreham, P. F. L., Galun, R. (1971). The origin of blood meals of female Culicoides pallidipennis trapped in a sheepfold in Israel. — J. med. Entomol. 8, 379381.
Carter, J. B.. (1973). Detection and assay of Tipula iridescent virus by the latex agglutination test. — J. gen. Virol. 21, 181185.
Cayzer, I., Whitehead, P. H.. (1973). The use of sensitised latex in the identification of human bloodstains. — J. forens. Sci. Soc. 13, 179181.
Eligh, G. S.. (1952). Factors influencing the performance of the precipitin test in the determination of blood meals of insects. — Can. J. Zool. 30, 213—218.
Lowry, O. H.., Rosebrough, N. J.., Farr, A. L., Randall, R. J.. (1951). Protein measurement with the folin phenol reagent. — J. biol. Chem. 193, 265275.
Murray, M. D.. (1970). The identification of blood meals in biting midges (Culicoides: Ceratopogonidae). — Ann. trop. Med. Parasit. 64, 115122.
Neville, E. M., Anderson, D.. (1972). Host preferences of Culicoides midges (Diptera: Ceratopogonidae) in South Africa as determined by precipitin tests and light trap catches. — Onderstepoort J. vet. Res. 39, 147152.
Pasolescu, O., Mihalcu, F.. (1975). A latex agglutination test for meningococcal infections diagnosis, pp. 439442 in Int. Symp. of Immunity to Infections of the Respiratory System in Man and Animals, London 1974. — Dev. biol. Standardisation 28, 656 pp.
Singer, J. M., Plotz, C. M.. (1956). The latex fixation test, for I: Application to the serological diagnosis of rheumatoid arthritis. — Am. J. Med. 21, 888892.
Steinbuch, M., Audran, R.. (1969). The isolation of IgG from mammalian sera with the aid of caprylic acid. — Archs. Biochem. Biophys. 134, 279284.
Tempelis, C. H.. (1975). Host-feeding patterns of mosquitoes, with a review of advances in analysis of blood meals by serology.— J. med. Entomol. 11, 635653.
Tempelis, C. H., Nelson, R. L.. (1971). Blood-feeding patterns of midges of the Culicoides variipennis complex in Kern County, California. — J. med. Entomol. 8, 532534.
Walker, A. R., Boreham, P. F. L.. (1976a). Blood feeding of Culicoides (Diptera, Ceratopogonidae) in Kenya in relation to the epidemiology of bluetongue and ephemeral fever. — Bull. ent. Res. 66, 181188.
Walker, A. R., Boreham, P. F. L.. (1976b). Saline as a collecting medium for Culicoides (Diptera, Ceratopogonidae) in blood feeding and other studies. — Mosquito News. 36, 1820.
Walker, A. R., Davies, F. G.. (1971). A preliminary survey of the epidemiology of bluetongue in Kenya. — J. Hyg. Camb. 69, 4761.
Whitehead, P. H.., Brech, A., Cayzer, I.. (1974). The identification of the species origin of bloodstains using sensitized latex. — J. Forens. Sci. Soc. 14, 103107.

A latex agglutination test for the identification of blood-meals of Culicoides (Diptera: Ceratopogonidae)

  • J. Boorman (a1), P. S. Mellor (a1), P. F. L. Boreham (a2) and Rosemary S. Hewett (a2)

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