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Geography has a greater effect than Wolbachia infection on population genetic structure in the spider mite, Tetranychus pueraricola

Published online by Cambridge University Press:  14 June 2016

Y.-T. Chen ,
Y.-K. Zhang ,
W.-X. Du ,
P.-Y. Jin  and
X.-Y. Hong
Y.-T. Chen
Affiliation:
Department of Entomology, Nanjing Agricultural University, Nanjing, Jiangsu, China
Y.-K. Zhang
Affiliation:
Department of Entomology, Nanjing Agricultural University, Nanjing, Jiangsu, China
W.-X. Du
Affiliation:
Department of Entomology, Nanjing Agricultural University, Nanjing, Jiangsu, China
P.-Y. Jin
Affiliation:
Department of Entomology, Nanjing Agricultural University, Nanjing, Jiangsu, China
X.-Y. Hong*
Affiliation:
Department of Entomology, Nanjing Agricultural University, Nanjing, Jiangsu, China
*
*Author for correspondence Fax: +86 25 84395339 Phone: +86 25 84395339 E-mail: xyhong@njau.edu.cn
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Abstract

Wolbachia is an intracellular symbiotic bacterium that infects various spider mite species and is associated with alterations in host reproduction, which indicates the potential role in mite evolution. However, studies of Wolbachia infections in the spider mite Tetranychus pueraricola, a major agricultural pest, are limited. Here, we used multilocus sequence typing to determine Wolbachia infection status and examined the relationship between Wolbachia infection status and mitochondrial diversity in T. pueraricola from 12 populations in China. The prevalence of Wolbachia ranged from 2.8 to 50%, and three strains (wTpue1, wTpue2, and wTpue3) were identified. We also found double infections (wTpue1 + wTpue3) within the same individuals. Furthermore, the wTpue1 strain caused weak cytoplasmic incompatibility (CI) (egg hatchability ~55%), whereas another widespread strain, wTpue3, did not induce CI. There was no reduction in mitochondrial DNA (mtDNA) or nuclear DNA diversity among infected individuals, and mtDNA haplotypes did not correspond to specific Wolbachia strains. Phylogenetic analysis and analysis of molecular variance revealed that the distribution of mtDNA and nuclear DNA haplotypes were significantly associated with geography. These findings indicate that Wolbachia infection in T. pueraricola is complex, but T. pueraricola genetic differentiation likely resulted from substantial geographic isolation.

Keywords

WolbachiaTetranychus pueraricolamtDNA6-phosphofructokinasepopulation structure
Type
Research Papers
Information
Bulletin of Entomological Research , Volume 106 , Issue 5 , October 2016 , pp. 685 - 694
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Copyright © Cambridge University Press 2016 

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