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Genetic variation among Helicoverpa armigera populations as assessed by microsatellites: a cautionary tale about accurate allele scoring

  • A.R. Weeks (a1), N.M. Endersby (a1), C.L. Lange (a2), A. Lowe (a3) (a4), M.P. Zalucki (a2) and A.A. Hoffmann (a1) (a5)...


The existence of genetic differences among Australian populations of the pest moth Helicoverpa armigera based on microsatellite markers is contentious. To resolve this issue, we analyzed microsatellite variation in moth samples from multiple locations simultaneously in two laboratories that have previously reported contrasting patterns. Alleles and allele numbers detected in the laboratories differed, as did the genetic differences found between the samples. The automated scoring system used in one of the laboratories combined with non-denaturing polyacrylamide gels led to inaccurate identification of alleles and high FST values between the populations. However, H. armigera in Australia is probably not structured geographically, with high gene flow between populations. This influences management of H. armigera and the development of area-wide control options, as populations need to be considered as one panmictic unit. The results also highlight potential problems of automated scoring systems when these are not checked carefully.


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Amornsak, W., Gordh, G. & Graham, G.C. (1998). Detecting parasitised eggs with polymerase chain reaction and DNA sequence of Trichogramma australicum Girault (Hymenoptera: Trichogrammatidae). Australian Journal of Entomology 37, 174179.
Daly, J.C. & Gregg, P. (1985) Genetic variation in Heliothis in Australia: species identification and gene flow in the two pest species H. armigera (Hubner) and H. punctigera Wallengren (Lepidoptera: Noctuidae). Bulletin of Entomological Research 75, 169184.
Endersby, N.M., McKechnie, S.W., Vogel, H., Gahan, L.J., Baxter, S.W., Ridland, P.M. & Weeks, A.R. (2005) Microsatellites isolated from diamondback moth, Plutella xylostella (L.), for studies of dispersal in Australian populations. Molecular Ecology Notes 5, 5153.
Endersby, N.M., Hoffmann, A.A., McKechnie, S.W. & Weeks, A.R. (2007) Is there genetic structure in populations of Helicoverpa armigera from Australia? Entomologia Experimentalis et Applicata 122, 253263.
Fitt, G.P. (1989) The ecology of Heliothis species in relation to agroecosystems. Annual Review of Entomology 34, 1752.
Goudet, J. (2001) FSTAT: a program to estimate and test gene diversities and fixation indices (version 2.9.3). (accessed 08 April 2009).
Ji, Y.-J., Zhang, D.-X., Hewitt, G.M., Kang, L. & Li, D.-M. (2003) Polymorphic microsatellite loci for the cotton bollworm Helicoverpa armigera (Lepidoptera: Noctuidae) and some remarks on their isolation. Molecular Ecology Notes 3, 102104.
Miller, S.A., Dykes, D.D. & Polesky, H.F. (1988) A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Research 16, 1215.
Sambrook, J. & Russell, D.W. (2001) Molecular Cloning: A Laboratory Manual, vol. 2, protocol 9, pp. 5.405.46. Cold Spring Harbor, New York, USA, Cold Spring Harbor Laboratory Press.
Scott, K.D., Wilkinson, K.S., Merritt, M.A., Scott, L.J., Lange, C.L., Schutze, M.K., Kent, J.K., Merritt, D.J., Grundy, P.R. & Graham, G.C. (2003) Genetic shifts in Helicoverpa armigera Hubner (Lepidoptera: Noctuidae) over a year in the Dawson/Callide Valleys. Australian Journal of Agricultural Research 54, 739744.
Scott, K.D., Lange, C.L., Scott, L.J. & Graham, G.C. (2004) Isolation and characterization of microsatellite loci from Helicoverpa armigera Hübner (Lepidoptera: Noctuidae). Molecular Ecology Notes 4, 204205.
Scott, K.D., Lawrence, N., Lange, C.L., Scott, L.J., Wilkinson, K.S., Merritt, M.A., Miles, M., Murray, D. & Graham, G.C. (2005a) Assessing moth migration and population structuring in Helicoverpa armigera (Lepidoptera: Noctuidae) at the regional scale: example from the Darling Downs, Australia. Journal of Economic Entomology 98, 22102219.
Scott, K.D., Wilkinson, K.S., Lawrence, N., Lange, C.L., Scott, L.J., Merritt, M.A., Lowe, A.J. & Graham, G.C. (2005b) Gene-flow between populations of cotton bollworm Helicoverpa armigera (Lepidoptera: Noctuidae) is highly variable between years. Bulletin of Entomological Research 95, 381392.
Scott, L.J., Lawrence, N., Lange, C.L., Graham, G.C., Hardwick, S., Rossiter, L., Dillon, M.L. & Scott, K.D. (2006) Population dynamics and gene flow of Helicoverpa armigera (Lepidoptera: Noctuidae) on cotton and grain crops in the Murrumbidgee Valley, Australia. Journal of Economic Entomology 99, 155163.
Spackman, M.E. & McKechnie, S.W. (1995) Assessing the value of mitochondrial DNA variation for detecting population subdivision in the cotton bollworm Helicoverpa armigera (Lepidoptera: Noctuidae), in Australia. pp. 811813 in Proceedings Beltwide Cotton Conferences. National Cotton Council of America, San Antonio, TX, USA.
Zhang, D.-X. (2004) Lepidopteran microsatellite DNA: redundant but promising. Trends in Ecology & Evolution 19, 507509.


Genetic variation among Helicoverpa armigera populations as assessed by microsatellites: a cautionary tale about accurate allele scoring

  • A.R. Weeks (a1), N.M. Endersby (a1), C.L. Lange (a2), A. Lowe (a3) (a4), M.P. Zalucki (a2) and A.A. Hoffmann (a1) (a5)...


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