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DNA barcoding and elucidation of cryptic aphid species (Hemiptera: Aphididae) in India

Published online by Cambridge University Press:  17 May 2013

K.B. Rebijith ,
R. Asokan ,
N.K. Krishna Kumar ,
V. Krishna ,
B.N. Chaitanya  and
V.V. Ramamurthy
K.B. Rebijith*
Affiliation:
Division of Biotechnology, Indian Institute of Horticultural Research, Bangalore, India
R. Asokan*
Affiliation:
Division of Biotechnology, Indian Institute of Horticultural Research, Bangalore, India
N.K. Krishna Kumar
Affiliation:
National Bureau of Agriculturally Important Insects (NBAII), Bangalore, India
V. Krishna
Affiliation:
Department of Biotechnology and Bioinformatics, Kuvempu University, Jnanasahyadri, Shankaraghatta, Shimoga, India
B.N. Chaitanya
Affiliation:
Division of Biotechnology, Indian Institute of Horticultural Research, Bangalore, India
V.V. Ramamurthy
Affiliation:
Division of Entomology, Indian Agricultural Research Institute (IARI), New Delhi, India
*
*Author for correspondence Phone: +91 80 28466420; Fax: +91 80 28466291 E-mail: rebijith@gmail.com, asokaniihr@gmail.com
*Author for correspondence Phone: +91 80 28466420; Fax: +91 80 28466291 E-mail: rebijith@gmail.com, asokaniihr@gmail.com
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Abstract

Rapid, precise and timely identification of invasive pest insects such as aphids is important and a challenge worldwide due to their complex life cycles, parthenogenetic reproduction, sex and colour morphs. In this respect, DNA barcoding employing a 658 bp fragment of 5′ region of the mitochondrial cytochrome oxidase I (CO-I) gene is an effective tool in addressing the above. In the present study, we employed CO-I for discriminating 142 individuals representing 32 species of aphids from India. Sequence analyses revealed that the intraspecific and interspecific distances ranged from zero to 3.8% and 2.31 to 18.9%, respectively. In addition, the study also showed for the first time the prevalence of three cryptic species, namely Brevicoryne brassicae (Linnaeus), Hyperomyzus carduellinus (Theobald) and Brachycaudus helichrysi (Kaltenbach) from India. Our work has clearly demonstrated that DNA barcoding is an efficient and accurate method for identification of aphid species (including cryptic species), an approach that potentially could play an important role in formulating viable pest management strategies, more especially biocontrol.

Keywords

aphidbiocontolCO-Icryptic speciescytochrome oxidaseDNA barcodinginvasives
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 103 , Issue 5 , October 2013 , pp. 601 - 610
Copyright
Copyright © Cambridge University Press 2013 

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Supplementary material: File

Rebijith Supplementary Material

Supplementary material 1. The interspecific genetic distances for all species of aphids employed in the current study.

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Supplementary material: File

Rebijith Supplementary Material

Supplementary material 2. The intergeneric divergence for all species of aphids employed in the current study.

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