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Distribution and dynamics of Bemisia tabaci invasive biotypes in central China

  • Q. Rao (a1) (a2), C. Luo (a2), H. Zhang (a1), X. Guo (a2) and G.J. Devine (a3)...

Abstract

The tobacco whitefly, Bemisia tabaci (Gennadius), causes severe crop losses in many agricultural systems. The worst of these losses are often associated with the invasion and establishment of specific whitefly biotypes. In a comprehensive survey of biotypes present in central China between 2005 and 2007, we obtained 191 samples of B. tabaci from 19 districts in Hubei province and its surrounds. Biotypes were identified by RAPD-PCR and by sequencing the mitochondrial cytochrome oxidase I gene (mtCO1). We determined that these central Chinese haplotypes included the world's two most invasive B. tabaci biotypes (B and Q) and two indigenous biotypes (ZHJ1 and ZHJ3). The B biotype shared >99.7% identity with other Chinese B biotypes and the Q biotype shared >99.5% of its identity with Q samples from the Mediterranean, USA, Africa and East Asia. By 2007, the Q biotype was dominant over much of Hubei province and appeared to be supplanting all other biotypes, although both the invasive and indigenous biotypes existed in sympatry in some regions. The invasion and rapid establishment of the Q biotype in China mirrors events elsewhere in the world, and we suggest that this is a consequence of its reproductive isolation, its polyphagous nature and its broad-spectrum resistance to insecticides. Its dominance has severe implications for the sustainability of some insecticide groups and for the production of a number of crops.

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*Authors for correspondence Fax: +8627 87396057 (H. Zhang); +8610 51503899 (C. Luo) E-mail: hongyu.zhang@mail.hzau.edu.cn; luochen1010@yahoo.com.cn

References

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Abdullahi, I., Atiri, G.I., Thottappilly, G. & Winter, S. (2004) Discrimination of cassava-associated Bemisia tabaci in Africa from polyphagous populations, by PCR-RFLP of the internal transcribed spacer regions of ribosomal DNA. Journal of Applied Entomology 128, 8187.
Bellows, T.S., Perring, T.M., Gill, R.J. & Headrick, D.H. (1994) Description of a species of Bemisia (Homoptera: Aleyrodidae). Annals of the Entomological Society of America 87, 195206.
Boykin, L.M., Shatters, R.G. Jr, Rosell, R.C., McKenzie, C.L., Bagnall, R.A., De Barro, P.J. & Frohlich, D.R. (2007) Global relationships of Bemisia tabaci (Hemiptera: Aleyrodidae) revealed using Bayesian analysis of mitochondrial CO1 DNA sequences. Molecular Phylogenetics and Evolution 44, 13061319.
Brown, J.K., Frohlich, D.R. & Rosell, R.C. (1995a) The sweetpotato or silverleaf whiteflies: biotypes of Bemisia tabaci or a species complex? Annual Review of Entomology 40, 511534.
Brown, J.K., Coats, S., Frohlich, D.R., Bedford, I.D. & Markham, P.G. (1995b) Characterization and distribution of esterase electromorphs in the whitefly, Bemisia tabaci (Genn.) (Homoptera: Aleyrodidae). Biochemical Genetics 33, 205214.
Brown, J.K., Perring, T.M., Cooper, A.D., Bedford, I.D. & Markham, P.G. (2000) Genetic analysis of Bemisia (Hemiptera: Aleyrodidae) populations by isoelectric focusing electrophoresis. Biochemical Genetics 38, 1325.
Byrne, D.N., Bellows, T.S. & Parrella, M.P. (1990) Whiteflies in agricultural systems. pp. 227262 in Gerling, D. (Ed.) Whiteflies: their Bionomics, Pest Status and Management. Intercept, Andover, UK.
Cervera, M.T., Cabezas, J.A., Simón, B., Martínez-Zapater, J.M., Beitia, F. & Cenis, J.L. (2000) Genetic relationships among biotypes of Bemisia tabaci (Hemiptera: Aleyrodidae) based on AFLP analysis. Bulletin of Entomological Research 90, 391396.
Chu, D., Chen, G.F., Xu, B.Y., Wu, Q.J. & Zhang, Y.J. (2007) RAPD analysis of population genetic structure of Bemisia tabaci biotype B and biotype Q. Acta Enotomologica Sinica 50, 264270.
Chu, D., Wan, F.H., Tao, Y.L., Liu, G.X., Fan, Z.X. & Bi, Y.P. (2008) Genetic differentiation of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) biotype Q based on mitochondrial DNA markers. Insect Science 15, 115123.
Costa, H.S. & Brown, J.K. (1991) Variation in biological characteristics and esterase patterns among populations of Bemisia tabaci, and the association of one population with silverleaf symptom induction. Entomologia Experimentalis et Applicata 61, 211219.
Dalmon, A., Halkett, F., Granier, M., Delatte, H. & Peterschmitt, M. (2008) Genetic structure of the invasive pest Bemisia tabaci: evidence of limited but persistent genetic differentiation in glasshouse populations. Heredity 100, 316325.
De Barro, P.J. & Driver, J.F. (1997) Use of RAPD-PCR to distinguish the B biotype from other biotypes of Bemisia tabaci (Gennathus) (Hemiptera: Aleyrodidae). Australian Journal of Entomology 36, 149152.
De Barro, P.J., Driver, F., Trueman, J.W.H. & Curran, J. (2000) Phylogenetic relationships of world populations of Bemisia tabaci (Gennadius) using ribosomal ITS1. Molecular Phylogenetics and Evolution 16, 2936.
Delatte, H., Reynaud, B., Granier, M., Thornary, L., Lett, J.M., Goldbach, R. & Peterschmitt, M. (2005) A new silverleaf-inducing biotype Ms of Bemisia tabaci (Hemiptera: Aleyrodidae) indigenous to the islands of the south-west Indian Ocean. Bulletin of Entomological Research 95, 2935.
Dinsdale, A., Cook, L., Riginos, C., Buckley, Y.M. & De Barro, P. (2010) Refined Global Analysis of Bemisia tabaci (Hemiptera: Sternorrhyncha: Aleyrodoidea: Aleyrodidae) Mitochondrial Cytochrome Oxidase 1 to Identify Species Level Genetic Boundaries. Annals of the Entomological Society of America 103, 196208.
Feng, L.X., Yang, Y.H., Xie, B.Y. & Yang, C.R. (2001) Likely epidermics of virus diseases in vegetable crops caused by outbreaks of Bemisia tabaci. China Vegetables 2, 3435.
Frohlich, D.R., Torres-Jerez, I., Bedford, I.D., Markham, P.G. & Brown, J.K. (1999) A phylogeographical analysis of the Bemisia tabaci species complex based on mitochondrial DNA markers. Molecular Ecology 8, 16831691.
Gawel, N.J. & Bartlett, A.C. (1993) Characterization of differences between whiteflies using RAPD-PCR. Insect Molecular Biology 2, 3338.
Horowitz, A.R., Gorman, K., Ross, G. & Denholm, I. (2003) Inheritance of Pyriproxyfen Resistance in the Whitefly, Bemisia tabaci (Q Biotype). Archives of Insect Biochemistry and Physiology 54, 177186.
Horowitz, A.R., Kontsedalov, S., Khasdan, V. & Ishaaya, I. (2005) Biotypes B and Q of Bemisia tabaci and their relevance to neonicotinoid and pyriproxyfen resistance. Archives of Insect Biochemistry and Physiology 58, 216225.
Horowitz, R., Kontsedalov, S., Khasdan, V., Breslauer, H. & Ishaaya, I. (2008) The biotypes B and Q of Bemisia tabaci in Israel-Distribution, resistance to insecticides and implications for pest management. Journal of Insect Science 8, 2324.
Hsieh, C.H., Wang, C.H. & Ko, C.C. (2007) Evidence from molecular markers and population genetic analyses suggests recent invasions of the western north pacific region by biotypes B and Q of Bemisia tabaci (Gennadius). Environmental Entomology 36, 952961.
Karunker, I., Benting, J., Lueke, B., Ponge, T., Nauen, R., Roditakis, E., Vontas, J., Gorman, K., Denholm, I. & Morin, S. (2008) Over-expression of cytochrome P450 CYP6CM1 is associated with high resistance to imidacloprid in the B and Q biotypes of Bemisia tabaci (Hemiptera: Aleyrodidae). Insect Biochemistry and Molecular Biology 38, 634644.
Kumar, S., Tamura, K. & Nei, M. (2004) MEGA3: Integrated software for molecular evolutionary genetic analysis and sequence alignment. Briefings in Bioinformatics 5, 150163.
Liu, S.S., De Barro, P.J., Xu, J., Luan, J.B., Zang, L.S., Ruan, Y.M. & Wan, F.H. (2007) Asymmetric mating interactions drive widespread invasion and displacement in a whitefly. Science 318, 17691772.
Luo, C. & Zhang, Z.L. (2000) Study progress on Bemisia tabaci (Gennadius). Beijing Agricultural Science (China) 18(Suppl.), 413.
Luo, C., Yao, Y., Wang, R.J., Yan, F.M., Hu, D.X. & Zhang, Z.L. (2002) The use of mitochondrial cytochrome oxidase I (mt CO1) sequences for identification biotype of Bemisia tabaci (Gennadius) in China. Acta Enotomologica Sinica 45, 759763.
Luo, C., Jones, C.M., Zhang, F., Denholm, I., Gorman, K. (2010) Insecticide-resistance in Bemisia tabaci biotype-Q from China. Crop Protection 29, 429434.
Ma, D.Y., Gorman, K., Devine, G., Luo, W.C. & Denholm, I. (2007) The biotype and insecticide-resistance status of whiteflies, Bemisia tabaci (Hemiptera: Aleyrodidae), invading cropping systems in Xinjiang Uygur Autonomous Region, northwestern China. Crop Protection 26, 612617.
Muniz, M. & Gloria, N. (2001) Differential variation in development of the B and Q biotypes of Bemisia tabaci (Homoptera: Aleyrodidae) on sweet pepper at constant temperatures. Environmental Entomology 30, 720727.
Nombela, G., Beitia, F. & Muniz, M. (2001) A differential interaction study of Bemisia tabaci Q-biotype on commercial tomato varieties with or without the mi resistance gene, and comparative host responses with the B-biotype. Entomologia Experimentalis et Applicata 98, 339344.
Oliveira, M.R.V., Henneberry, T.J. & Anderson, P. (2001) History, current status, and collaborative research projects for Bemisia tabaci. Crop Protection 20, 709723.
Perring, T.M. (2001) The Bemisia tabaci species complex. Crop Protection 20, 725737.
Perring, T.M., Cooper, A.D., Rodriguez, R.J., Farrar, C.A. & Bellows, T.S. Jr (1993) Identification of a whitefly species by genomic and behavioral studies. Science 259, 7477.
Qiu, B.L., Ren, S.X., Wen, S.Y. & Mandour, N.S. (2003) Biotype identification of the populations of Bemisia tabaci (Hemiptera: Aleyrodidae) in China using RAPD-PCR. Acta Enotomologica Sinica 46, 605608.
Rekha, A.R., Maruthi, M.N., Muniyappa, V. & Colvin, J. (2005) Occurrence of three genotypic clusters of Bemisia tabaci and the rapid spread of the B biotype in south India. Entomologia Experimentalis et Applicata 117, 221233.
Simon, C., Frati, F., Beckenbach, A., Crespi, B., Lui, H. & Flook, P. (1994) Evolution, weighting, and phylogenetic utility of mitochondirial gene sequences and a compilation of conserved polymerase chain reaction ‘primers’. Annals of the Entomological Society of America 87, 651701.
Sseruwagi, P., Legg, J.P., Maruthi, M.N., Colvin, J., Rey, M.E.C. & Brown, J.K. (2005) Genetic diversity of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) populations and presence of the B biotype and a non-B biotype that can induce silverleaf symptoms in squash, in Uganda. The Annals of Applied Biology 147, 253265.
Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F. & Higgins, D.G. (1997) The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 24, 48764882.
Yokomi, R.K., Hoelmer, K.A. & Osborne, L.S. (1990) Relationship between the sweetpotato whitefly and the squash silverleaf disorder. Phytopathology 80, 895900.
Zang, L.S., Liu, S.S., Liu, Y.Q., Ruan, Y.M. & Wan, F.H. (2005a) Competition between the B biotype and non-B biotype of the whitefly Bemisia tabaci (Homoptera: Aleyrodidae) in Zhejiang, China. Chinese Biodiversity 13, 181187.
Zang, L.S., Liu, S.S., Liu, Y.Q. & Chen, W.Q. (2005b) A comparative study on the morphological and biological characteristics of the B biotype and a non-B biotype (China-ZHJ-1) of Bemisia tabaci (Homoptera: Aleyrodidae) from Zhejiang, China. Acta Enotomologica Sinica 48, 742748.
Zang, L.S., Jiang, T., Xu, J., Liu, S.S. & Zhang, Y.J. (2006) SCAR molecular markers of the B biotype and two non-B populations of the whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae). Journal of Agricultural Biotechnology 14, 208212.
Zhang, L.P., Zhang, Y.J., Zhang, W.J., Wu, Q.J., Xu, B.Y. & Chu, D. (2005) Analysis of genetic diversity among different geographical populations and determination of biotypes of Bemisia tabaci in China. Journal of Applied Entomology 129, 121128.
Zhou, G.Z. & Dong, C.S. (2006) Investigation on the occurrence and damage of Bemisia tabaci on vegetable in Hubei. Hubei Plant Protection(China) 2, 1920.

Keywords

Distribution and dynamics of Bemisia tabaci invasive biotypes in central China

  • Q. Rao (a1) (a2), C. Luo (a2), H. Zhang (a1), X. Guo (a2) and G.J. Devine (a3)...

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