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Contrast in the cuticular hydrocarbons of sympatric Phlebotomus (Synphlebotomus) females (Diptera: Phlebotominae)

  • T. Gebre-Michael (a1), R. P. Lane (a2), A. Phillips (a3), P. Milligan (a3) and D. H. Molyneux (a3)...

Abstract

The cuticular hydrocarbons of laboratory-bred and wild-caught females of Phlebotomus martini Parrot and P. celiae Minter, originating from southern Ethiopia, were used to distinguish females of the two sympatric and isomorphic species. Field-caught sandflies were dissected for parasites prior to the analysis. A high degree of separation, was achieved between the two species in both laboratory-bred (91.6%) and wild-caught (91.3%) specimens. However, the discriminating hydrocarbon peaks in laboratory reared specimens were different from those of wild-caught specimens, resulting in a high number of misclassiflcations in the pooled analysis of wild and laboratory specimens in each species. High degrees of hydrocarbon differentiation (84.7%–96.7%) between laboratory-bred and wild caught flies within each species were also obtained.

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Corresponding author

Department of Entomology, The Natural History Museum, Cromwell Road, London SW7 5BD, UK.

References

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Ayele, T. & Ali, A. (1984) The distribution of visceral leishmaniasis in Ethiopia. American Journal of Tropical Medicine and Hygiene 33, 548552.
Blomquist, G.J. & Jackson, L.L. (1973) Incorporation of labelled dietary n-alkenes into cuticular lipids of the grasshopper, Melanopus sanguinipes. Journal of Insect Physiology 19, 16391647.
Carlson, D.A. & Service, M.W. (1990) Identification of mosquitoes of Anopheles gambiae species complex A and B by analysis of cuticular hydrocarbons. Science 207, 10891091.
Gebre-Michael, T. & Lane, R.P. (1991) Scanning electron microscopy of eggs of Phlebotomus (Synphlebotomus) martini and P. (Syn.) celiae (Diptera: Phlebotominae). Parassitologia 33, (Suppl. 1) 261266.
Gebre-Michael, T. & Lane, R.P. (1993a) Distinguishing the females of Phlebotomus (Synphlebotomus) martini and P. (S.) celiae (Diptera, Phlebotominae), vectors of visceral leishmaniasis in southern Ethiopia. Bulletin of Entomological Research. 83, 353360.
Gebre-Michael, T., Lane, R.P., Frame, I.A. & Miles, M.A. (1993b) Leishmania donovani infections in phlebotomine sandflies from the kala azar focus at Aba Roba in Ethiopia: DNA probe compared with conventional detection methods. Medical and Veterinary Entomology 7, 294296.
Hadley, N.F. (1977) Epicuticular lipids of the desert tenebrionid beetle Eleodes aramata: seasonal and acclimatory effects of composition. Insect Biochemistry 7, 277283.
Hoppe, K.L., Dillwith, J., Wright, R.E. & Szumlas, D.E. (1990) Identification of horseflies (Diptera: Tabanidae) by analysis of cuticular hydrocarbons. Journal of Medical of Entomology 27, 481486.
Howard, R.W. & Blomquist, G.J. (1982) Chemical ecology and biochemistry of insect hydrocarbons. Annual Review of Entomology 27, 149172.
Howard, R.W., Thorne, B.L., Levings, S.C. & McDaniel, C.A. (1988) Cuticular hydrocarbons as chemotaxonomic characters for Nasutitermes corniger (Motschulsky) and N. ephratae (Holmgren) (Isoptera: Termitidae). Annals of the Entomological Society of America 81, 395399.
Jackson, L.L. (1974) Cuticular lipids of adult fleshflies, Sarcophaga bullata. Insect Biochemistry 4, 369379.
Kamhawi, S., Lane, R.P., Cameron, M., Phillips, A., Milligan, P. & Molyneux, D.H. (1992) The cuticular hydrocarbons of Phlebotomus argentipes (Diptera: Phlebotominae) from field populations in northern India and Sri Lanka and their change with laboratory colonization. Bulletin of Entomological Research 82, 209212.
Lockey, K.H. (1976) Cuticular hydrocarbons of Locusta, Schistocerca and Periplaneta and their role in waterproofing. Insect Biochemistry 6, 457472.
Milligan, P.J.M., Phillips, A., Molyneux, D.H., Subbarao, S.K. & White, G.B. (1986) Differentiation of Anopheles culicifacies Giles (Diptera: Culicidae) sibling species by analysis of cuticular components. Bulletin of Entomological Research 76, 529537.
Page, M., Nelson, L.J., Haverty, M.I. & Blomquist, G.J. (1990) Cuticular hydrocarbons as chemotaxonomic characters for bark beetles: Dendroctonus ponderosae, D. Jeffreyi, D. brevicomis, and D. frontalis (Coleoptera: Scolytidae). Annals of the Entomological Society of America 83, 893901.
Philips, A., Le Pont, F, Desjeux, P., Broomfield, G., Molyneux, D.H. (1990a) Separation of Psychodopygus carrerai carrerai and P. yucumensis (Diptera Psychodidae) by gas chromatography of cuticular hydrocarbons. Acta Tropica 47, 145149.
Phillips, A., Milligan, P.J.M., Maroli, M., Lane, R.P., Kamhawi, S., Broomfield, G. & Molyneux, D.H. (1990b) Intraspecific variation in cuticular hydrocarbons of the sandfly Phlebotomus perfiliewi from Italy. Medical and Veterinary Entotmology 4, 451457.
Phillips, A., Walsh, J.F., Garms, R., Molyneux, D.H., Mulligan, P. & Ibrahim, G.H. (1985) Identification of adults of the Simulium damnosum complex using hydrocarbon analysis. Tropenmedizin und Parasitologie 36, 97101.
Pomoni, J.G. & Mackley, J. (1985) Gas chromatographic composition profiles of surface lipid extracts from screwworm compared by age, sex, colonization and geography. Southwestern Entomologist 10, 6576.
Ryan, L., Phillips, A., Milligan, P., Lainson, R., Molyneux, D.H. & Shaw, J.J. (1986) Separation of femal Psychodopygus wellcomei and P. complexus (Diptera: Psychodidae) by cuticular hydrocarbon analysis. Acta Tropica 43, 8589.
Silhacek, D.L., Carlson, D.A., Mayer, M.S. & James, J.D. (1972) Composition and sex attractancy of cuticular hydrocarbons from houseflies: effects of age, sex and mating. Journal of Insect Physiology 18, 347354.
Toolson, E.C. (1982) Effects of rearing temperature on cuticle permeability and epicuticular lipid composition in Drosophila pseudoobscura. Journal of Experimental Zoology 222, 249253.
Toolson, E.C. & Hadley, N.F. (1979) Seasonal effects on cuticular permeability and epicuticular lipid composition in Centruroides sculpturatus Ewing 1928 (Scorpiones: Buthidae). Journal of Comparative Physiology 129, 319325.
Toolson, E.C. & Kuper-Simbron, R. (1989) Laboratory evolution of epicuticular hydrocarbon composition and cuticular permeability in Drosophila pseudoobscura: effects on sexual dimorphism and thermal-acclimation ability. Evolution 43, 468473.

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