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Cloning of three epsilon-class glutathione S-transferase genes from Micromelalopha troglodyta (Graeser) (Lepidoptera: Notodontidae) and their response to tannic acid

Published online by Cambridge University Press:  08 February 2024

Ling Zhang ,
Huizhen Tu  and
Fang Tang Open the ORCID record for Fang Tang [Opens in a new window]
Ling Zhang
Affiliation:
Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, People's Republic of China College of Forestry, Nanjing Forestry University, Nanjing 210037, People's Republic of China
Huizhen Tu
Affiliation:
Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, People's Republic of China College of Forestry, Nanjing Forestry University, Nanjing 210037, People's Republic of China
Fang Tang*
Affiliation:
Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, People's Republic of China College of Forestry, Nanjing Forestry University, Nanjing 210037, People's Republic of China
*
Corresponding author: Fang Tang; Email: tangfang76@sohu.com
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Abstract

Micromelalopha troglodyta (Graeser) is an important pest of poplar in China, and glutathione S-transferase (GST) is an important detoxifying enzyme in M. troglodyta. In this paper, three full-length GST genes from M. troglodyta were cloned and identified. These GST genes all belonged to the epsilon class (MtGSTe1, MtGSTe2, and MtGSTe3). Furthermore, the expression of these three MtGSTe genes in different tissues, including midguts and fat bodies, and the MtGSTe expression in association with different concentrations of tannic acid, including 0.001, 0.01, 0.1, 1, and 10 mg ml−1, were analysed in detail. The results showed that the expression levels of MtGSTe1, MtGSTe2, and MtGSTe3 were all the highest in the fourth instar larvae; the expression levels of MtGSTe1 and MtGSTe3 were the highest in fat bodies, while the expression level of MtGSTe2 was the highest in midguts. Furthermore, the expression of MtGSTe mRNA was induced by tannic acid in M. troglodyta. These studies were helpful to clarify the interaction between plant secondary substances and herbivorous insects at a deep level and provided a theoretical foundation for controlling M. troglodyta.

Keywords

expressionglutathione S-transferaseMicromelalopha troglodyta (Graeser)tannic acid
Type
Research Paper
Information
Bulletin of Entomological Research , First View , pp. 1 - 8
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Copyright
Copyright © The Author(s), 2024. Published by Cambridge University Press

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Footnotes

*

Both authors contributed equally to this study.

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