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Characterization, expression profiling, and thermal tolerance analysis of heat shock protein 70 in pine sawyer beetle, Monochamus alternatus hope (Coleoptera: Cerambycidae)

Published online by Cambridge University Press:  16 September 2020

Hui Li ,
Heng Qiao ,
Yujie Liu ,
Shouyin Li ,
Jiajin Tan  and
Dejun Hao Open the ORCID record for Dejun Hao [Opens in a new window]
Hui Li
Affiliation:
Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China College of Forestry, Nanjing Forestry University, Nanjing, China
Heng Qiao
Affiliation:
Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China College of Forestry, Nanjing Forestry University, Nanjing, China
Yujie Liu
Affiliation:
Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China College of Forestry, Nanjing Forestry University, Nanjing, China
Shouyin Li
Affiliation:
Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China College of Forestry, Nanjing Forestry University, Nanjing, China
Jiajin Tan
Affiliation:
Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China College of Forestry, Nanjing Forestry University, Nanjing, China
Dejun Hao*
Affiliation:
Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China College of Forestry, Nanjing Forestry University, Nanjing, China
*
Author for correspondence: Dejun Hao, Email: djhao@njfu.edu.cn
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Abstract

Monochamus alternatus Hope (Coleoptera: Cerambycidae) warrants attention as a dominant transmission vector of the pinewood nematode, and it exhibits tolerance to high temperature. Heat shock protein 70 (HSP70) family members, including inducible HSP70 and heat shock cognate protein 70 (HSC70), are major contributors to the molecular chaperone networks of insects under heat stress. In this regard, we specifically cloned and characterized three MaltHSP70s and three MaltHSC70s. Bioinformatics analysis on the deduced amino acid sequences showed these genes, having close genetic relationships with HSP70s of Coleopteran species, collectively shared conserved signature structures and ATPase domains. Subcellular localization prediction revealed the HSP70s of M. alternatus were located not only in the cytoplasm and endoplasmic reticulum but also in the nucleus and mitochondria. The transcript levels of MaltHSP70s and MaltHSC70s in each state were significantly upregulated by exposure to 35–50°C for early 3 h, while MaltHSP70s reached a peak after exposure to 45°C for 2–3 h in contrast to less-upregulated MaltHSC70s. In terms of MaltHSP70s, the expression threshold in females was lower than that in males. Also, both fat bodies and Malpighian tubules were the tissues most sensitive to heat stress in M. alternatus larvae. Lastly, the ATPase activity of recombinant MaltHSP70-2 in vitro remained stable at 25–40°C, and this recombinant availably enhanced the thermotolerance of Escherichia coli. Overall, our findings unraveled HSP70s might be the intrinsic mediators of the strong heat tolerance of M. alternatus due to their stabilized structure and bioactivity.

Keywords

ATPase activityexpression analysisheat shock protein 70Monochamus alternatusthermotolerance
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 111 , Issue 2 , April 2021 , pp. 217 - 228
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Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

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