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Assessing common bean genotypes for resistance to Diabrotica speciosa (Coleoptera: Chrysomelidae)

Published online by Cambridge University Press:  19 June 2023

Eduardo Neves Costa Open the ORCID record for Eduardo Neves Costa [Opens in a new window] ,
Elias Soares Gomes ,
Lucas Coutinho Reis ,
Andressa Caroline Foresti ,
Silvana de Paula Quintão Scalon  and
Marcos Gino Fernandes Open the ORCID record for Marcos Gino Fernandes [Opens in a new window]
Eduardo Neves Costa*
Affiliation:
Universidade Federal da Grande Dourados, Faculdade de Ciências Agrárias, Programa de Pós-graduação em Agronomia, Rodovia Dourados/Itahum, Km 12, 79.804-970, Dourados, MS, Brazil, Universidade Federal da Grande Dourados, Faculdade de Ciências Biológicas e Ambientais, Programa de Pós-Graduação em Entomologia e Conservação da Biodiversidade, Rodovia Dourados/Itahum, Km 12, 79.804-970, Dourados, MS, Brazil
Elias Soares Gomes
Affiliation:
Universidade Federal da Grande Dourados, Faculdade de Ciências Biológicas e Ambientais, Programa de Pós-Graduação em Entomologia e Conservação da Biodiversidade, Rodovia Dourados/Itahum, Km 12, 79.804-970, Dourados, MS, Brazil
Lucas Coutinho Reis
Affiliation:
Universidade Federal da Grande Dourados, Faculdade de Ciências Agrárias, Programa de Pós-graduação em Agronomia, Rodovia Dourados/Itahum, Km 12, 79.804-970, Dourados, MS, Brazil,
Andressa Caroline Foresti
Affiliation:
Universidade Federal da Grande Dourados, Faculdade de Ciências Agrárias, Programa de Pós-graduação em Agronomia, Rodovia Dourados/Itahum, Km 12, 79.804-970, Dourados, MS, Brazil,
Silvana de Paula Quintão Scalon
Affiliation:
Universidade Federal da Grande Dourados, Faculdade de Ciências Agrárias, Programa de Pós-graduação em Agronomia, Rodovia Dourados/Itahum, Km 12, 79.804-970, Dourados, MS, Brazil,
Marcos Gino Fernandes
Affiliation:
Universidade Federal da Grande Dourados, Faculdade de Ciências Agrárias, Programa de Pós-graduação em Agronomia, Rodovia Dourados/Itahum, Km 12, 79.804-970, Dourados, MS, Brazil, Universidade Federal da Grande Dourados, Faculdade de Ciências Biológicas e Ambientais, Programa de Pós-Graduação em Entomologia e Conservação da Biodiversidade, Rodovia Dourados/Itahum, Km 12, 79.804-970, Dourados, MS, Brazil
*
Corresponding author: Eduardo Neves Costa; Email: costa_ne@yahoo.com.br
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Abstract

Diabrotica speciosa (Germar) (Coleoptera: Chrysomelidae) is a major pest of common bean (Phaseolus vulgaris L.; Fabales: Fabaceae), and adults can defoliate plants during the whole crop cycle. This study was conducted to evaluate the resistance to D. speciosa in 16 common bean genotypes (14 landraces and 2 cultivars), through three different experiments. In the laboratory, choice and no-choice feeding tests were performed to evaluate the percentage of leaf consumption. In the greenhouse, plant height, numbers of leaves, percentage of injured leaves, percentage of injury per leaf, weight of seeds, and D. speciosa survival were evaluated. Furthermore, trichome density, levels of peroxidase (POD), superoxide dismutase (SOD), and protein content in common bean leaves were assessed. In the laboratory, the genotypes Chumbinho Branco, Dobalde, Manteigado, IPR Tuiuiú, and 90D Mouro were the least consumed by D. speciosa. In the greenhouse, the genotypes Dobalde, Manteigado, and IPR Tuiuiú expressed tolerance to the pest, which was associated with a higher plant height and/or unchanged POD and SOD levels and protein content following insect feeding, and no reduction in seed production. The landrace 90D Mouro exhibited antixenosis and tolerance to D. speciosa, observed as a lower leaf injury, higher trichome density, lower protein contents, higher SOD level and no reduction in seed weight. Overall, we have shown that antixenosis and tolerance can help overcome damages resulting from D. speciosa feeding, with emphasis on four common bean genotypes that may be useful in plant breeding programs aimed at controlling D. speciosa in common bean crops.

Keywords

Antixenosisbean leaf beetleperoxidasePhaseolus vulgaris Lsuperoxide dismutasetolerance
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 113 , Issue 4 , August 2023 , pp. 546 - 554
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Copyright
Copyright © The Author(s), 2023. Published by Cambridge University Press

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