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Toll-interacting protein participates in immunity and development of the lepidopteran insect Antheraea pernyi

Published online by Cambridge University Press:  06 June 2023

Zhe Chen
Affiliation:
College of Life Sciences, Anhui Agricultural University, Hefei 230036, China
Awei Zhang
Affiliation:
College of Life Sciences, Anhui Agricultural University, Hefei 230036, China
Xuan Xu
Affiliation:
College of Life Sciences, Anhui Agricultural University, Hefei 230036, China
Lu Ding
Affiliation:
College of Life Sciences, Anhui Agricultural University, Hefei 230036, China
Xiaojiao Zhang
Affiliation:
College of Life Sciences, Anhui Agricultural University, Hefei 230036, China
Cen Qian
Affiliation:
College of Life Sciences, Anhui Agricultural University, Hefei 230036, China
Baojian Zhu*
Affiliation:
College of Life Sciences, Anhui Agricultural University, Hefei 230036, China
*
Corresponding author: Baojian Zhu; Email: zhubaojian@ahau.edu.cn

Abstract

Toll-interacting protein (Tollip) participates in multiple biological processes. However, the biological functions of Tollip proteins in insects remain to be further explored. Here, the genomic sequence of tollip gene from Antheraea pernyi (named Ap-Tollip) was identified with a length of 15,060 bp, including eight exons and seven introns. The predicted Ap-Tollip protein contained conserved C2 and CUE domains and was highly homologous to those tollips from invertebrates. Ap-Tollip was highly expressed in fat body compared with other determined tissues. As far as the developmental stages were concerned, the highest expression level was found at the 14th day in eggs or the 3rd day of the 1st instar. Ap-Tollip was also obviously regulated by lipopolysaccharide, polycytidylic acid or 20E in different tissues. In addition, the interaction between Ap-Tollip and ubiquitin was confirmed by western blotting and pull-down assay. RNAi of Ap-Tollip significantly affected the expression levels of apoptosis and autophagy-related genes. These results indicated that Ap-Tollip was involved in immunity and development of A. pernyi.

Type
Research Paper
Copyright
Copyright © The Author(s), 2023. Published by Cambridge University Press

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Footnotes

*

These authors contributed equally to this work.

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