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Genetic diversity and insecticide resistance during the growing season in the green peach aphid (Hemiptera: Aphididae) on primary and secondary hosts: a farm-scale study in Central Chile

Published online by Cambridge University Press:  03 February 2014

J.A. Rubiano-Rodríguez
Affiliation:
Facultad de Ciencias Agrarias, Universidad de Talca, Casilla 747, Talca, Chile
E. Fuentes-Contreras
Affiliation:
Facultad de Ciencias Agrarias, Universidad de Talca, Casilla 747, Talca, Chile
C.C. Figueroa
Affiliation:
Instituto de Biología Vegetal y Biotecnología, Universidad de Talca, Casilla 747, Talca, Chile Instituto de Ciencias Ambientales y Evolutivas, Facultad de Ciencias, Universidad Austral de Chile, Casilla 567, Valdivia, Chile
J.T. Margaritopoulos
Affiliation:
Department of Biochemistry and Biotechnology, University of Thessaly, Ploutonos 26 & Aiolou Street, 412 21 Larissa, Greece
L.M. Briones
Affiliation:
Instituto de Biología Vegetal y Biotecnología, Universidad de Talca, Casilla 747, Talca, Chile
C.C. Ramírez
Affiliation:
Instituto de Biología Vegetal y Biotecnología, Universidad de Talca, Casilla 747, Talca, Chile Millennium Nucleus Center in Molecular Ecology and Evolutionary Applications in the Agroecosystems
Corresponding
E-mail address:

Abstract

The seasonal dynamics of neutral genetic diversity and the insecticide resistance mechanisms of insect pests at the farm scale are still poorly documented. Here this was addressed in the green peach aphid Myzus persicae (Sulzer) (Hemiptera: Aphididae) in Central Chile. Samples were collected from an insecticide sprayed peach (Prunus persica L.) orchard (primary host), and a sweet-pepper (Capsicum annum var. grossum L.) field (secondary host). In addition, aphids from weeds (secondary hosts) growing among these crops were also sampled. Many unique multilocus genotypes were found on peach trees, while secondary hosts were colonized mostly by the six most common genotypes, which were predominantly sensitive to insecticides. In both fields, a small but significant genetic differentiation was found between aphids on the crops vs. their weeds. Within-season comparisons showed genetic differentiation between early and late season samples from peach, as well as for weeds in the peach orchard. The knock-down resistance (kdr) mutation was detected mostly in the heterozygote state, often associated with modified acetylcholinesterase throughout the season for both crops. This mutation was found in high frequency, mainly in the peach orchard. The super-kdr mutation was found in very low frequencies in both crops. This study provides farm-scale evidence that the aphid M. persicae can be composed of slightly different genetic groups between contiguous populations of primary and secondary hosts exhibiting different dynamics of insecticide resistance through the growing season.

Type
Research Paper
Copyright
Copyright © Cambridge University Press 2014 

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Genetic diversity and insecticide resistance during the growing season in the green peach aphid (Hemiptera: Aphididae) on primary and secondary hosts: a farm-scale study in Central Chile
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Genetic diversity and insecticide resistance during the growing season in the green peach aphid (Hemiptera: Aphididae) on primary and secondary hosts: a farm-scale study in Central Chile
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