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Artificial larviposition sites for field collections of the puparia of tsetse flies Glossina pallidipes and G. m. morsitans (Diptera: Glossinidae)

Published online by Cambridge University Press:  09 March 2007

M.O. Muzari  and
J.W. Hargrove
M.O. Muzari*
Affiliation:
National Institute of Health Research, Box CY573, Causeway, Harare, Zimbabwe
J.W. Hargrove
Affiliation:
National Institute of Health Research, Box CY573, Causeway, Harare, Zimbabwe
*
*Fax: 263 4 253979, E-mail: odwellmuzari@yahoo.com
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Abstract

Tsetse flies Glossina pallidipes Austen and G. morsitans morsitans Westwood deposit their larvae in warthog burrows, in August–November, at Rekomitjie Research Station, Zambezi Valley, Zimbabwe. Artificial burrows, made from 200–l steel drums, were used to sample these flies and to collect their puparia. Sand-filled plastic trays in the burrows served as a substrate for larval deposition. The sand was covered with c. 2 cm of leaf litter after it was shown that only 3% of larvae were deposited on bare sand if both substrates were available. Other burrow modifications – artificially shading the burrow entrance, increasing the relative humidity inside the burrow, or reducing the size of the burrow entrance – significantly decreased deposition rates. The use of burrows in the hot season results in a reduction in the temperature experienced by the puparium towards an assumed optimum level of 26°C. Artificial burrows maintained a mean temperature of 28.5°C during October-November 1998, c. 2.5°C cooler than ambient; earlier work has shown that natural burrows can be c. 5°C cooler than ambient at these times. This may explain why natural burrows in full sunlight were used for larviposition, whereas artificial burrows were used only when they were in deep shade, and why significantly higher proportions of G. pallidipes were found in natural (66%) than in artificial burrows (34%). Better-insulated artificial burrows might produce more puparia with higher proportions of G. pallidipes. Burrows become waterlogged during the rains and may be too cool for optimum puparial development during the rest of the year. The percentages of G. m. morsitans in catches of females from artificial burrows, refuges and odour-baited traps were 34, 26 and < 10% respectively. Traps are biased in favour of G. pallidipes; artificial burrows may show a bias in favour of G. m. morsitans that is a function of temperature. Artificial warthog burrows provide a convenient way of studying the puparial stage in tsetse and for the first time facilitate the capture of females as they deposit their larvae.

Keywords

TsetseGlossina pallidipesGlossina morsitanslarvipositionpupariawarthogburrowsZimbabwe
Type
Research Article
Information
Bulletin of Entomological Research , Volume 95 , Issue 3 , June 2005 , pp. 221 - 229
Copyright
Copyright © Cambridge University Press 2005

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References

Buxton, P.A. (1955) The natural history of tsetse flies, xviii + 816pp. London: H.K.Lewis.Google Scholar
Hargrove, J.W. (1981) Discrepancies between estimates of tsetse fly populations using mark–recapture and removal trapping techniques. Journal of Applied Ecology 50, 351–373.CrossRefGoogle Scholar
Hargrove, J.W. (1999a) Nutritional levels of female tsetse Glossina pallidipes from artificial refuges. Medical and Veterinary Entomology 13, 150–164.CrossRefGoogle ScholarPubMed
Hargrove, J.W. (1999b) Lifetime changes in the nutritional characteristics of female tsetse flies Glossina pallidipes caught in odour-baited traps. Medical and Veterinary Entomology 13, 165–176.CrossRefGoogle ScholarPubMed
Hargrove, J.W. (2001) The effect of temperature and saturation deficit on mortality in populations of male Glossina m. morsitans in Zimbabwe and Tanzania. Bulletin of Entomological Research 91, 79–86.CrossRefGoogle ScholarPubMed
Hargrove, J.W. (2004) Tsetse population dynamics. pp. 113–137in Maudlin, I., Holmes, P.H., Miles, P.H.. (Eds). The trypanosomiases. Wallingford, Oxon, CABI Publishing.CrossRefGoogle Scholar
Hargrove, J.W. & Brady, J. (1992) Activity rhythms of tsetse flies (Glossina spp.) (Diptera: Glossinidae) at low and high temperatures in nature. Bulletin of Entomological Research 82, 321–326.CrossRefGoogle Scholar
Hargrove, J.W. & Langley, P.A. (1990) Sterilizing tsetse in the field: a successful field trial. Bulletin of Entomological Research 80, 397–403.CrossRefGoogle Scholar
Jackson, P.J. & Phelps, R.J. (1967) Temperature regimes in pupation sites of Glossina morsitans orientalis Vanderplank (Diptera). Rhodesia, Zambia and Malawi Journal of Agricultural Research 5, 249–260.Google Scholar
Leegwater van der Linden, M.E. (1983) The rearing of the tsetse fly Glossina pallidipes Austen. 138 pp. PhD thesis, University of Amsterdam.Google Scholar
Muzari, M.O. & Hargrove, J.W. (1996) The design of target barriers for tsetse flies, Glossina spp. (Diptera: Glossinidae). Bulletin of Entomological Research 86, 579–583.CrossRefGoogle Scholar
Phelps, R.J. (1973) The effect of temperature on fat consumption during the puparial stages of Glossina morsitans morsitans Westw. (Dipt., Glossinidae) under laboratory conditions, and its implication in the field. Bulletin of Entomological Research 62, 423–438.CrossRefGoogle Scholar
Phelps, R.J. & Burrows, P.M. (1969) Prediction of the puparial duration in Glossina morsitans orientalis Vanderplank under field conditions. Journal of Applied Ecology 6, 323–337.CrossRefGoogle Scholar
Phelps, R.J. & Vale, G.A. (1978) Studies on populations of Glossina morsitans morsitans and G. pallidipes (Diptera: Glossinidae) in Rhodesia. Journal of Applied Entomology 15, 743–760.Google Scholar
Phelps, R.J., Burrows, P.M. & Boyd-Clark, M.J. (1967) Pre-emergence sexing of Glossina morsitans orientalis Vanderplank (Diptera) puparia. Rhodesia, Zambia and Malawi Journal of Agricultural Research 5, 267–271.Google Scholar
Phelps, R.J. & Simmonds, A.M. & Parsons, R. (1966) Pupal collection and respiratory physiology. Rhodesia Agricultural Research Council Annual Report 1966. pp. 77–79. Salisbury, Rhodesia.Google Scholar
Pilson, R.D. & Pilson, B.M. (1967) Behaviour studies of Glossina morsitans in the field. Bulletin of Entomological Research 57, 227–257.CrossRefGoogle ScholarPubMed
Rogers, D.J. (1974) Natural regulation and movement of tsetse fly populations. pp. 35–38 in Les Moyens de lutte contre les trypanosomes et leur vecteurs. Paris, Institut Elevage Medicale Veterinaire Pays Tropicale.Google Scholar
Rogers, D.J. (1990) A general model for tsetse populations. Insect Science and its Application 11, 331–346.Google Scholar
Rogers, D.J. & Randolph, S.E. (1990) Estimation of rates of predation on tsetse. Medical and Veterinary Entomology 4, 195–204.CrossRefGoogle ScholarPubMed
Saini, R.K., Hassanali, A., Andoke, J., Ahuya, P. & Ouma, W.P. (1996) Identification of major components of larviposition pheromone from larvae of tsetse flies Glossina morsitans morsitans Westwood Glossina morsitans centralis Machado. Journal of Chemical Ecology 22, 1211–1220.CrossRefGoogle Scholar
Torr, S.J. & Hargrove, J.W. (1999) Behaviour of tsetse (Diptera: Glossinidae) during the hot season in Zimbabwe: an interaction of micro-climate and reproductive status. Bulletin of Entomological Research 89, 365–379.CrossRefGoogle Scholar
Torr, S.J. & Mangwiro, T.N.C. (2000) Interactions between cattle and biting flies: effects on the feeding rate of tsetse. Veterinary Entomology 14, 400–409.CrossRefGoogle ScholarPubMed
Vale, G.A. (1971) Artificial refuges for tsetse flies (Glossina spp.). Bulletin of Entomological Research 61, 331–350.CrossRefGoogle Scholar
Vale, G.A. & Phelps, R.J. (1978) Sampling problems with tsetse flies (Diptera: Glossinidae). Journal of Applied Ecology 15, 715–726.CrossRefGoogle Scholar