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Use of in vitro digestion by protease from Streptomyces griseus, ficin or pancreatin for the estimation of ruminal effective degradability of dried lucerne forage

  • A. Antoniewicz (a1) and I. Kosmala (a1)

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Lucerne forage can provide significant amounts of both degraded and undegraded protein to ruminants. Changes in chemical composition taking place during advancement of maturity also affect ruminal degradability of protein. Therefore, forages harvested in a wide range of vegetation stages represent a good set of samples suitable to test and validate in vitro methods. The aim of the study was to elaborate the best conditions of using protease from Streptomyces griseus (PSG) to estimate ruminal degradability of dried lucerne forage and compare the results with those obtained when the same material was digested with ficin (FIC) or pancreatin (PNC).

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Antoniewicz, A. M. and Kosmala, I. 1995. Predicting ruminal degradability of lucerne and grass forage protein from in vitro solubility with non-specific bacterial protease or pancreatic Journal of Animal and Feed Sciences 4: 341350.
Aufrere, J. and Cartailler, D. 1988. Mise au point d'une methods de laboratoire de prevision de la degradabilite des proteines alimentaires dans le rumen. Annales de Zootechnie 37: 255270.
Blackburn, T. H. 1968. Protease production by Bacteroides amylophilus strain H18. Journal of General Microbiology 53: 2736.
Kosmala, I., Antoniewicz, A., De Boever, J., Hvelplund, T. and Kowalczyk, J. 1996. Use of enzymatic solubility with ficin (EC 3.4.22.3) to predict in situ feed protein degradability. Animal Feed Science and Technology 59: 245254.
Wallace, R. J. and Joblin, K. N. 1985. Proteolytic activity of a rumen anaerobic fungus Neocallimastix frontalis . FEMS Microbiological Letters 29: 1925.

Use of in vitro digestion by protease from Streptomyces griseus, ficin or pancreatin for the estimation of ruminal effective degradability of dried lucerne forage

  • A. Antoniewicz (a1) and I. Kosmala (a1)

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