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Potential application of cellulase and hemicellulase assay techniques for assessing the forage quality and performance of rumen micro-organisms

Published online by Cambridge University Press:  27 February 2018

M. K. Bhat
M. K. Bhat*
Affiliation:
Institute of Food Research, Reading Laboratory, Earley Gate, Whiteknights Road, Reading RG6 6BZ
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Extract

Cellulose and hemicellulose are the major structural polysaccharides of plant cell wall. The efficient utilization of these polysaccharides by ruminants is often restricted by the presence of lignin. Cellulose and hemicellulose are hydrolysed by a group of enzymes called cellulases and hemicellulases. The present paper describes the cellulase and hemicellulase assay methods and their potential applications.

Carboxymethyl (CM)-cellulose, Avicel, cellobiose, xylobiose, p-nitrophenyl-p β-D-glucoside (pNPG), p-nitrophenyl-β-D-cellobioside (pNPC), p-nitrophenyl-β-D-xyloside (pNPX) and p-nitrophenyl- α-L-arabinofuranoside (pNPAf) were from Sigma. Birchwood xylan and filter paper are from Carl Roth GmbH and Co., Germany and Whatman International Ltd, UK, respectively. H3P04-Swollen cellulose and 4-O-methyl-α-D-glucuronyl-xylotriose (mGpA-Xyl3) were prepared as described (Wood, 1988; Khandke et al., 1989a).

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Information
BSAP Occasional Publication , Volume 22: In vitro techniques for measuring nutrient supply to ruminants , 1998 , pp. 290 - 293
Copyright
Copyright © British Society of Animal Science 1998

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References

Bailey, M. J., Biely, P. and Poutanen, K. 1992. Interlaboratory testing of methods for assay of xylanase activity. Journal of Biotechnology 23:257–270.Google Scholar
Bhat, S., Wallace, R. J. and Ørskov, E. R. 1988. Study of the relation between straw quality and its colonization by rumen micro-organisms. Journal of Agricultural Science 110:561–565.Google Scholar
Bhat, S., Wallace, R. J. and Ørskov, E. R. 1990. Adhesion of cellulolytic rumen bacteria to barley straw. Applied and Environmental Microbiology 56:2698–2703.Google Scholar
Deshpande, M. V., Eroksson, K.-E. and Pettersson, L. G. 1984. An assay of selective determination of exo-l,4-b-glucanase in a mixture of cellulolytic enzymes. Analytical Biochemistry 138:481–487.Google Scholar
Dubois, M., Gilles, K., Hamilton, J. K., Rebers, P. A. and Smith, F. 1956. Colorimetric method for determination of sugars and related substances. Analytical Biochemistry 28:350–356.Google Scholar
Khandke, K., Vithayathil, P. J. and Murthy, S. K. 1989a. Purification and characterisation of an a-D-glucuronidase from a thermophilic fungus, T. Aurantiacus . Archives of Biochemistry and Biophysics 274:511–517.Google Scholar
Khandke, K., Vithayathil, P. J. and Murthy, S. K. 1989b. Degradation of larchwood xylan by enzymes of a thermophilic fungus, T. Aurantiacus . Archives of Biochemistry and Biophysics 274:501–510.Google Scholar
Mandels, M., Andreotti, R. and Roche, C. 1976. Measurement of saccharifying cellulase. Biotechnology Bioengineering Symposium 6:17–23.Google Scholar
Milner, Y. and Avigard, G. 1967. A copper reagent for the determination of hexuronic acids and certain ketohexoses. Carbohydrate Research 4:359–361.Google Scholar
Smith, D. C, Bhat, M. K. and Wood, T. M. 1991. Xylan-hydrolysing enzymes from thermophilic and mesophilic fungi. World Journal of Microbiology and Biotechnology 7:475–484.Google Scholar
Somogyi, M. 1952. Notes on sugar determination. Journal of Biological Chemistry 195:19–23.Google Scholar
Sugden, C. and Bhat, M. K. 1994. Cereal straw and pure cellulose as carbon sources for growth and production of plant cell-wall degrading enzymes by S. thermophile . World Journal of Microbiology and Biotechnology 10:444–451.Google Scholar
Wood, T. M. 1988. Preparation of crystalline, amorphous and dyed cellulose substrates. Methods in Enzymology 160:19–25.CrossRefGoogle Scholar
Wood, T. M. and Bhat, M. K. 1988. Measuring cellulose activities. Methods in Enzymology 160:87–11 Google Scholar