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Modification of an in vitro batch culture technique to improve precision in long-term studies

  • M. C. Hickey (a1) (a2), A. P. Moloney (a1), M. O'Connell (a2) and J. Connolly (a3)

Extract

In vitro techniques have been developed to facilitate the measurement of nutritional variability amongst food. Many kinetic studies have utilized the modified Tilley and Terry technique, with long-term incubations carried out in Erlenmeyer flasks. These are inefficient in utilizing incubator space for large scale studies. However substitution of Erlenmeyer flasks with tubes as fermentation units leaves the system prone to ‘bridging’, the formation of dense mats of forage particles by entrapped gas, above the level of the media in a fermentation unit. The objective of experiment 1 was to establish an effective incubation technique to eliminate the random variation caused by bridging.

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References

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Dhanoa, M. S. 1988. Research note: on the analysis of dacron bag data for low degradability feeds. Grass and Forage Science 43: 441444.
Grant, R. J. and Mertens, D. R. 1992. Impact of in vitro fermentation techniques upon kinetics of fiber digestion. Journal of Dairy Science 75: 12631272.
Goering, H. K. and Van Soest, P. J. 1970. Forage fiber analysis (apparatus, reagents, procedures and some applications). Agricultural handbook no. 379, Agricultural Research Service, US Department of Agriculture, Washington, DC.
Luchini, N. D., Broderick, G. A. and Combs, D. K. 1996. Preservation of ruminal microorganisms for in vitro determination of ruminal protein degradation. Journal of Animal Science 74: 1134 1143.
Pell, A. N. and Schofield, P. 1993. Computerized monitoring of gas production to measure forage digestion in vitro . Journal of Dairy Science 76: 10631073.
Steel, R. G. D. and Torrie, J. H. 1960. Principles and procedures of statistics. McGraw-Hill Book Co., Inc., New York.

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