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Maternal influence on oocyte collection and embryo development after transvaginal puncture and in vitro fertilisation in dairy cows

Published online by Cambridge University Press:  27 February 2018

S. Chastant-Maillard ,
H. Quinton ,
C. Douar ,
J. Marchal ,
C. Richard ,
Y. Heyman  and
J.P. Renard
S. Chastant-Maillard
Affiliation:
LEGSA, Service de Reproduction, Ecole Nationale Vétérinaire d'Alfort, 94704 MAISONS-ALFORT, France
H. Quinton
Affiliation:
LEGSA, Service de Reproduction, Ecole Nationale Vétérinaire d'Alfort, 94704 MAISONS-ALFORT, France
C. Douar
Affiliation:
LEGSA, Service de Reproduction, Ecole Nationale Vétérinaire d'Alfort, 94704 MAISONS-ALFORT, France
J. Marchal
Affiliation:
Domaine de Bressonvilliers, INRA, 91630 LEUDEVILLE, France
C. Richard
Affiliation:
Domaine de Bressonvilliers, INRA, 91630 LEUDEVILLE, France
Y. Heyman
Affiliation:
LEGSA, Service de Reproduction, Ecole Nationale Vétérinaire d'Alfort, 94704 MAISONS-ALFORT, France
J.P. Renard
Affiliation:
LEGSA, Service de Reproduction, Ecole Nationale Vétérinaire d'Alfort, 94704 MAISONS-ALFORT, France
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Abstract

The aim of the study was to evaluate differences between cows in the “quality” of their oocytes defined as their ability to support embryonic development. Ten cows from the same herd, all primiparous and non-pregnant were submitted for oocyte collection by Ovum-Pick Up (OPU). The oocytes were matured in vitro and fertilised with semen from the same bull. In vitro embryo development, both quantitatively (percentages showing cleavage and forming blastocysts) and qualitatively (differential cell counting in blastocysts) was determined at the blastocyst stage (Day 7). The number of oocytes collected, the number of blastocysts obtained and the blastocyst formation rate varied between cows (P<0.001). The mean percentage of inner cell mass cells tended to higher for embryos derived from one cow. These results provide evidence that the quality of the oocytes was influenced by their maternal origin. Follicular growth also varied between cows.

Type
Posters
Information
BSAP Occasional Publication , Volume 26 , Issue 2: Fertility in the High-Producing Dairy Cow , September 2001 , pp. 433 - 435
Copyright
Copyright © British Society of Animal Science 2001

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References

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