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In vitro techniques as tools to predict nutrient supply in ruminants

  • S. Tamminga (a1) and B. A. Williams (a1)

Abstract

In vitro techniques are widely used to predict the nutritive value of foods for farm animals. However, food evaluation systems are moving towards systems based on nutrient flows rather than on energy or protein. Nutrients are supplied via the digestive tract and presently available in vitro methods are inadequate to simulate the complicated and non-steady-state processes in this tract. This is partly due the lack of adequate in vivo reference values and partly due to a too-high degree of standardization and simplification. Future developments should therefore aim to develop in vitro systems which closely monitor the dynamics of the digestive processes. Adequate interpretation of the results may require sophisticated mathematical models.

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Agricultural and Food Research Council. 1992. Technical Committee on Responses to Nutrients. Report no. 9. Nutritive requirements of ruminant animals: protein. Nutrition Abstracts and Reviews 62: 787835.
Antoniewicz, A. M., Vuuren, A. M. van, , Koelen, C. J. van der, and Kosmala, I. 1992. Intestinal digestibility of rumen undegraded protein of formaldehyde-treated feedstuffs measured by mobile bag and in vitro technique. Animal Feed Science and Technology 39:111124.
Aufrère, J., Graviou, D., Demarquilly, C, Vérité, R., Michalet-Doreau, B. and Chapoutot, R. 1991. Predicting in situ degradability of feed proteins in the rumen by two laboratory methods (solubility and enzymatic degradation). Animal Feed Science and Technology 33:97116.
Beuvink, J. M. W. and Kogut, J. 1993.Modelling gas production kinetics of grass silages incubated with buffered ruminal fluid, journal of Animal Science 71:10411046.
Beuvink, J. M. W., Spoelstra, S. F. and Hogendorp, R. J. 1992. An automated method for measuring the time course of gas production of feedstuffs incubated with buffered rumen fluid. Netherlands journal of Agricultural Science 40: 401407.
Boever, L. de, , Cottyn, B. G., Buysse, F. X., Wainman, F. W. and Vanacker, J. M. 1986. The use of an enzymatic technique to predict digestibility, metabolizable and net energy of compound feedstuffs for ruminants. Animal Feed Science and Technology 14:203214.
Boisen, S. and Eggum, B. O. 1991. Critical evaluation of in vitro methods for estimating digestibility in simple-stomach animals. Nutrition Research Reviews 4: 141162.
Broderick, G. A. and Merchen, N. R. 1992. Markers for quantifying microbial protein synthesis in the rumen. Journal of Dairy Science 75:26182632.
Broderick, G. A., Wallace, R. J. and Ørskov, E. R. 1992. Control of rate and extent of protein degradation. In Physiological aspects of digestion and metabolism in ruminants (ed. T., Tsuda, Y., Sasaki and R., Kawashimz), pp. 541592. Academic Press Inc., San Diego.
Bruchem, J. van, , Bongers, L. J. G. M., Walsum, J. D. van, , Onck, W. and Adrichem, P. W. M. van, . 1985. Digestion of proteins of varying degradability in sheep. 3. Apparent and true digestibility in the small intestine and ileal endogenous flow of N and amino acids. Netherlands Journal of Agricultural Science 33:285295.
Chen, G., Sniffen, C. J. and Russell, J. B. 1987. Concentration and estimated flow of peptides from the rumen of dairy cattle: effects of protein quantity, protein solubility and feeding frequency. Journal of Dairy Science 70: 12111219.
Clark, J. H., Klusmeyer, T. H. and Merchen, M. R. 1992. Microbial protein synthesis and flows of nitrogen fractions to the duodenum of dairy cows. Journal of Dairy Science 75: 23042323.
Cone, J. W. 1991. Degradation of starch in feed concentrates by enzymes, rumen fluid and rumen enzymes. Journal of the Science of Food and Agriculture 54:2334.
Cone, J. W. 1996. Influence of rumen fluid and substrate concentration on fermentation kinetics measured with a fully automated time related gas production apparatus. Animal Feed Science and Technology 61:113128.
Czerkawski, J. W. 1986. An introduction to rumen studies. Pergamon Press, Oxford.
Dado, R. G. and Allen, M. S. 1995. Intake limitations, feeding behaviour and rumen function of cows challenged with rumen fill from dietary fiber or inert bulk. Journal of Dairy Science 78:118133.
Dijkstra, J., Boer, H., Bruchem, J. van, , Bruining, M. and Tamminga, S. 1993. Absorption of volatile fatty acids from the rumen of lactating dairy cows as influenced by volatile fatty acid concentration, pH and rumen liquid volume. British Journal of Nutrition 69:385396.
Forbes, J. M. 1994. Voluntary feed intake and diet selection in farm animals. CAB International, Wallingford, UK.
Forbes, J. M. 1995. Physical limitations of feed intake in ruminants and its interactions with other factors affecting intake. In Ruminant physiology: digestion, metabolism, growth and reproduction(ed. W. v., Engelhardt, Leonhard-Marek, S., Breves, G. and Giesecke, D.), pp. 217232. Ferdinand Enke Verlag, Stuttgart.
France, J., Dhanoa, M. S., Theodorou, M. K., Lister, S. J., Davies, D. R. and Isaac, D. 1993. A model to interpret gas accumulation profiles associated with in vitro degradation of ruminant feeds. Journal of Theoretical Biology 163:99111.
France, J. and Siddons, R. C. 1993. Volatile fatty acid production. In Quantitative aspects of ruminant digestion and metabolism(ed.Forbes, J. M. and France, J.), pp. 107122. CAB International.
Groot, J. C. J., Cone, J. W., Williams, B. A. and Debersaques, F. 1996. Multiphasic analysis of gas production kinetics on in vitro ruminal fermentation. Animal Feed Science and Technology 64:7789.
Groot, J. C. J., Williams, B. A., Oostdam, A. J., Boer, H. and Tamminga, S. 1998. The use of cumulative gas and volatile fatty acid production to predict in vitro fermentation kinetics of Italian ryegrass leaf cell walls and content at various time intervals. British Journal of Nutrition 79: 519525.
Harmon, D. L. 1992. Dietary influences on carbohydrases and small intestinal starch hydrolysis capacity in ruminants. Journal of Nutrition 122:203210.
Harmon, D. L. 1993. Nutritional regulation of postruminal digestive enzymes in ruminants. Journal of Dairy Science 76: 21022111.
Hvelplund, T. 1991. Volatile fatty acids and protein production in the rumen. In Rumen microbial metabolism and ruminant digestion, pp.165178. INRA, Paris.
Illg, D. J. and Stern, M. D. 1994. In vitro and in vivo comparisons of diaminopimelic acid and purines for estimating protein synthesis in the rumen. Animal Feed Science and Technology 48:4955.
Ketelaars, J. J. M. H. and Tolkamp, B. J. 1991. Toward a new theory of feed intake regulation in ruminants. Ph.D. thesis, Wageningen Agricultural University.
Kosmala, I., Antoniewicz, A., Boever, J. de, , Hvelplund, T. and Kowalczyk, J. 1996. Use of enzymatic solubility with ficin (EC 3.4.22.3) to predict in situ feed protein degradability. Animal Feed Science and Technology 59: 245 254.
Lindsay, D. B. 1993. Metabolism of the portal drained viscera. In Quantitative aspects of ruminant digestion and metabolism(ed. Forbes, J. M. and France, J.), pp. 267290. CAB International, Wallingford, UK.
Menke, K. H., Raab, L., Salewski, A., Steingass, H., Fritz, D. and Schneider, W. 1979. The estimation of the digestibility and metabolizable energy content of ruminant feedstuffs from the gas production when they are incubated with rumen liquor. Journal of Agricultural Science, Cambridge 93:217222.
Menke, K. H. and Steingass, H. 1988. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Animal Research and Development 28:755.
Murphy, M. R., Baldwin, R. L. and Koong, L. J. 1982. Estimation of stoichiometric parameters for rumen fermentation of roughage and concentrate diets. Journal of Animal Science 55:411421.
Neal, H. D. St C., Dijkstra, J. and Gill, M. 1992. Simulation of nutrient digestion, absorption and outflow in the rumen: model evaluation. Journal of Nutrition 122:22572272.
Nocek, J. E. and Tamminga, S. 1991. Site of digestion of starch in the gastrointestinal tract of dairy cows and its effect on milk yield and composition. Journal of Dairy Science 74:35983629.
O'Connor, J. D., Robinson, P. H., Sniffen, C. J. and Allen, M. S. 1984. A gastro-intestinal tract simulation model of digesta flow in ruminants. In Techniques in particle size analysis of feed and digesta in ruminants(ed. P. M. Kennedy), pp. 102122. Canadian Society of Animal Production, occasional publication no.1.
Ørskov, E. R., Macleod, N. A. and Kyle, D. J. 1986. Flow of nitrogen from the rumen and abomasum in cattle and sheep given protein-free nutrients by intragastric infusion. British Journal of Nutrition 56:241248.
Owens, F. N. and Goetsch, A. L. 1986. Digesta passage and microbial protein synthesis. In Control of digestion and metabolism in ruminants (ed. Milligan, L. P., Grovum, W. L. and Dobson, A.), pp.196223. Prentice Hall, New York.
Pell, A. N. and Schofield, P. J. 1993. Computerized monitoring of gas production to measure forage digestion in vitro . Journal of Dairy Science 76:10631073.
Poos-Floyd, M., Klopfenstein, T. and Britton, R. A. 1985. Evaluation of laboratory techniques for predicting ruminal protein degradation. Journal of Dairy Science 68:829839.
Roe, M. B., Chase, L. A. and Sniffen, C. J. 1991. Comparison of in vitro techniques to the in situ technique for estimation of ruminal degradation of protein. Journal of Dairy Science 74:16321640.
Rulquin, H., Pisulewski, P. M., Vérité, R. and Guinard, J. 1993. Milk production and composition as a function of postruminal lysine and methionine supply: a nutrient response approach. Livestock Production Science 37:6990.
Russell, J. B., O'Connor, J. D., Fox, D. G., Van Soest, P. J. and Sniffen, C. J. 1992. A net carbohydrate and protein system for evaluating cattle diets. I. Ruminal fermentation. Journal of Animal Science 70:35513561.
Sauvant, D., Chapoutot, P. and 1994. La digestion des amidons par les ruminants et ses consequences. INRA Production Animates 7/2:115124.
Schofield, P., Pitt, R. E. and Pell, A. N. 1994. Kinetics of fibre digestion from in vitro gas production. Journal of Animal Science 72: 29802991.
Storm, E. and Ørskov, E. R. 1983. The nutritive value of rumen micro-organisms in ruminants. 1. Large scale isolation and chemical composition of rumen microorganisms. British Journal of Nutrition 50:463470.
Straalen, W. M. van, , Odinga, J. J. and Mostert, W. 1997. Digestion of feed amino acids in the rumen and small intestine of dairy cows measured with nylon-bag techniques. British Journal of Nutrition 77: 8397.
Straalen, W. M. van, . 1995. Modelling of nitrogen flow and excretion in dairy cows. Ph.D. thesis, Wageningen Agricultural University, The Netherlands.
Sutton, J. D. 1985. Digestion and absorption of energy substrates in the lactating cow. Journal of Dairy Science 68: 33763393.
Tamminga, S. 1993. Influence of feeding management on ruminant fiber digestibility. In Forage cell wall structure and digestibility(ed. Jung, H.-J. G., Buxton, R. D., Hatfield, R. D. and Ralph, J.), pp. 571602. American Society of Agronomy, Madison, WI.
Tamminga, S., Robinson, P. H., Meijs, S. and Boer, H. 1989a. Feed components as internal markers in digestion studies with dairy cows. Animal Feed Science and Technology 27: 4957.
Tamminga, S., Robinson, P. H., Vogt, M. and Boer, H. 1989b. Rumen ingesta kinetics of cell wall components and nitrogen in dairy cows. Animal Feed Science and Technology 25: 8998.
Tamminga, S., Straalen, M. W. van, , Subnel, A. P. J., Meijer, R. G. M., Steg, A., Wever, C. J. G. and Blok, M. C. 1994. The Dutch protein evaluation system; the DVE/OEBsystem. Livestock Production Science 40:139155.
Theodorou, M. K., Williams, B. A., Dhanoa, M. S., McAllan, A. B. and France, J. 1994. A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds. Animal Feed Science and Technology 48:185197.
Tilley, J. M. A. and Terry, R. A. 1963. A two-stage technique for the in vitro digestion of forage crops. Journal of the British Grassland Society 18:104111.
Van Soest, P. J. 1994. Nutritional ecology of the ruminant. Cornell University Press, Ithaca.
Vuuren, A. M. van, . 1993. Digestion and nitrogen metabolism of grass fed dairy cows. Ph.D. thesis, Wageningen Agricultural University.
Williams, B. A., Bhatia, S. K., Boer, H. and Tamminga, S. 1995. A preliminary study using the cumulative gas production technique to compare the kinetics of different fermentations by use of standard substrates. Annates de Zootechnie 44: (supplement): 35.
Williams, B. A., Boer, H., Diekema, B. and Tamminga, S. 1994, A progressive carbon balance for the in vitro fermentation of wheat straw, using the gas production technique. Proceedings of the Society of Nutritional Physiology 3:184.

In vitro techniques as tools to predict nutrient supply in ruminants

  • S. Tamminga (a1) and B. A. Williams (a1)

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