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Effect of inoculum from suckling rabbits of different ages on fermentation parameters obtained with the in vitro gas production technique

Published online by Cambridge University Press:  27 February 2018

M.P. Gazaneo ,
F. Bovera ,
C. Di Meo ,
G Piccolo  and
A. Nizza
M.P. Gazaneo
Affiliation:
Dipartimento di Scienze Zootecniche e Ispezione degli Alimenti, Università “Federico II”, Via F. Delpino 1 – 80137Napoli, Italy
F. Bovera
Affiliation:
Dipartimento di Scienze Zootecniche e Ispezione degli Alimenti, Università “Federico II”, Via F. Delpino 1 – 80137Napoli, Italy
C. Di Meo
Affiliation:
Dipartimento di Scienze Zootecniche e Ispezione degli Alimenti, Università “Federico II”, Via F. Delpino 1 – 80137Napoli, Italy
G Piccolo
Affiliation:
Dipartimento di Scienze Zootecniche e Ispezione degli Alimenti, Università “Federico II”, Via F. Delpino 1 – 80137Napoli, Italy
A. Nizza
Affiliation:
Dipartimento di Scienze Zootecniche e Ispezione degli Alimenti, Università “Federico II”, Via F. Delpino 1 – 80137Napoli, Italy
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Summary

The in vitro gas production technique was used to observe the fermentation characteristics of three feedstuffs (barley, dried alfalfa meal, and dried beet pulp). Inocculum was provided from the caecal contents of suckling rabbits (23, 26, 31 and 35d old) which had access to their dams’ food. Mean OM degradation increased progressively with the age of the rabbits (P<0.01) from 67.4 to 70.8% with inocculum from 23 and 35d kits, and gas production from 302 to 357 ml/g OM incubated (P<0.01). It was confirmed that the in vitro gas production technique is a valid tool for the study the development of caecal microbial activity in rabbits.

Resumen

Resumen

Se observaron las caracteristicas de fermentación de tres alimentos (cebada, hierba médica deshidratada y pulpa de remolacha) a través de la ‘técnica de producción de gas in vitro’, usando como inoculo contenido cecal de conejos lactantes a diferentes edades, 23, 26, 31 y 35 días los cuales tuvieron acceso a la comida de sus madres. De los resultados obtenidos se observa que con el incremento de la edad del animal, fuente del inoculo, se aumenta progresivamente la materia orgánica degradada (67.4 vs 70.8%, respectivamente de 23 y 35 días de edad), y, por consiguiente aumenta la producción de gas (302 vs 357 ml/g de materia orgánica incubada). La técnica de producción de gas in vitro se confirma, por lo tanto, como un instrumento válido por el estudio de la evolución de la actividad microbal en el ciego del conejo.

Type
Research Article
Information
BSAP Occasional Publication , Volume 34: Herbivores: Assessment of intake, digestibility and the roles of secondary compounds , 2006 , pp. 63 - 68
Copyright
Copyright © British Society of Animal Science 2006

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References

AOAC, (1984). Official Methods of Analysis, 14th edition. Association of Official Analytical Chemists, Washington, DC, USA.Google Scholar
Calabrò, S., Nizza, A., Pinna, W., Cutrignelli, M.I. and Piccolo, V. (1999). Estimation of digestibility of compound diets for rabbits using the in vitro gas production technique. World Rabbit Science 7: 197–201 Google Scholar
Calabrò, S., Nizza, A., Sanna, C. and Piccolo, V. (2000a). Fermentation Kinetics of some feedstuffs for rabbits using the in vitro gas production technique. Proceedings 7th World Rabbit Congress, Valencia, Spain, pp 145–152 Google Scholar
Calabrò, S., Piccolo, V., Infascelli, F., Bovera, F. and Cutrignelli, M.I. (2000b). Fermentation Kinetics of some by–products with the in vitro gas production technique. Bubalus bubalus 1: 65–74 Google Scholar
Groot, J.C.J., Cone, J.W., Williams, B.A., Debersaques, F.M.A. and Lantinga, E.A. (1996). Multiphasic analysis of gas production kinetics for in vitro fermentation of ruminant feedstuffs. Animal Feed Science and Technology 64: 77–89 CrossRefGoogle Scholar
Kwakkel, R.P., Williams, B.A. and Van Der Poel, A.F.B. (1997). Effects of fine and coarse particle diets on gizzard growth and fermentation characteristics of the caecal contents in broiler chicken. In: 11th European Symposium on Poultry Nutrition (WPSA). Faaborg, Denmark, pp. 32–36 Google Scholar
Macheboeuf, D., Jestin, M. and Martin-Rosset, W. (1998). Utilisation of the gas test method using horse faeces as a source of inoculum‥ In vitro techniques for measuring nutrient supply to ruminants In:Proceedings International Symposium British Society of Animal Science, Occasional Publication No 22: pp. 187– 189 Google Scholar
Padilha, M.T.S., Licois, D., Gidenne, T., Carre, B. and Fonty, G. (1995). Relationships between microflora and caecal fermentation in rabbit before and after weaning. Reproduction Nutrition Development 3: 375–386 CrossRefGoogle Scholar
Pell, A.N. and Schofield, P. (1993). Computerized monitoring of gas production to measure forage digestion in vitro. Journal of Dairy Science 76: 1063–1073 CrossRefGoogle ScholarPubMed
Theodorou, M.K. (1993). A new laboratory procedure for determining the fermentation kinetics of ruminants feeds. Ciencia e Investigation Agraria 20: 332–344.IGoogle Scholar
Theodorou, M.K., Williams, B.A., Dhanoa, M.S., McAllan, A.B. and France, J. (1994). A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminal feeds. Animal Feed Science and Technology 48: 185–197 CrossRefGoogle Scholar
Williams, B.A., Voigt, C. and Verstegen, M.W.A. (1998). The faecal microbial population representative of large intestinal microfloral activity. In: Proceedings International Symposium. “In vitro techniques for measuring nutrient supply to ruminants” British Society of Animal Science, Occasional Publication No 22: pp. 165 Google Scholar