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The effect of head-space pressure on gas production profiles

Published online by Cambridge University Press:  27 February 2018

R. S. Lowman ,
A. E. Brooks ,
M. K. Theodorou ,
M. S. Dhanoa  and
D. Cuddeford
R. S. Lowman
Affiliation:
Department of Veterinary Clinical Studies, Edinburgh University, Veterinary Field Station, Easter Bush, Roslin, Midlothian EH20 9RG Institute of Grassland and Environmental Research, Plus Gogerddan, Aberystwyth SY23 3EB
A. E. Brooks
Affiliation:
Institute of Grassland and Environmental Research, Plus Gogerddan, Aberystwyth SY23 3EB
M. K. Theodorou
Affiliation:
Institute of Grassland and Environmental Research, Plus Gogerddan, Aberystwyth SY23 3EB
M. S. Dhanoa
Affiliation:
Institute of Grassland and Environmental Research, Plus Gogerddan, Aberystwyth SY23 3EB
D. Cuddeford
Affiliation:
Department of Veterinary Clinical Studies, Edinburgh University, Veterinary Field Station, Easter Bush, Roslin, Midlothian EH20 9RG
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Extract

In vitro gas production techniques are becoming increasingly popular as tools to investigate the food quality for ruminant and monogastric herbivores. They are attractive due to their ability to measure the kinetics of digestion associated with the gut microbial fermentation of foodstuffs. It is therefore important to know and understand what factors affect the volume of gas produced, particularly as they relate to repeatability within or between laboratories. In studies involving gas pressure and volume measurement, the chemical composition of the culture medium, the nature of the microbial population and the type and amount of substrate available for fermentation all have a significant influence on gas accumulation. In this study, the manual pressure transducer technique (PTT) of Theodorou et al. (1994) was used to investigate the effect of head-space pressure on the resultant gas production profiles.

Type
Posters
Information
BSAP Occasional Publication , Volume 22: In vitro techniques for measuring nutrient supply to ruminants , 1998 , pp. 205 - 206
Copyright
Copyright © British Society of Animal Science 1998

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References

Davies, D. R., Theodorou, M. K., Baughan, J., Brooks, A. E. and Newbold, J. R. 1995. An automated pressure evaluation system (APES) for determining the fermentation characteristics of ruminant feeds. Fourth international symposium on the nutrition of herbivores, Cleremont-Ferrand, ll-15th September 1995. Annates de Zootechnie 44: 36 (abstr.).CrossRefGoogle Scholar
Theodorou, M. K., Williams, B. A., Dhanoa, M. S., McAllan, A. B. and France, J. 1994. A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds. Animal Feed Science and Technology 48:185–197.CrossRefGoogle Scholar