Skip to main content Accessibility help
×
Home

Quantitative analyses of Streptococcus mutans biofilms with quartz crystal microbalance, microjet impingement and confocal microscopy

  • J. Kreth (a1), E. Hagerman (a2), K. Tam (a3), J. Merritt (a4), D. T. W. Wong (a1), B. M. Wu (a2) (a5), N. V. Myung (a3), W. Shi (a1) (a4) and F. Qi (a1)...

Abstract

Microbial biofilm formation can be influenced by many physiological and genetic factors. The conventional microtiter plate assay provides useful but limited information about biofilm formation. With the fast expansion of the biofilm research field, there are urgent needs for more informative techniques to quantify the major parameters of a biofilm, such as adhesive strength and total biomass. It would be even more ideal if these measurements could be conducted in a real-time, non-invasive manner. In this study, we used quartz crystal microbalance (QCM) and microjet impingement (MJI) to measure total biomass and adhesive strength, respectively, of S. mutans biofilms formed under different sucrose concentrations. In conjunction with confocal laser scanning microscopy (CLSM) and the COMSTAT software, we show that sucrose concentration affects the biofilm strength, total biomass, and architecture in both qualitative and quantitative manners. Our data correlate well with previous observations about the effect of sucrose on the adherence of S. mutans to the tooth surface, and demonstrate that QCM is a useful tool for studying the kinetics of biofilm formation in real time and that MJI is a sensitive, easy-to-use device to measure the adhesive strength of a biofilm.

Copyright

Corresponding author

Corresponding author: Dr F. Qi, Department of Oral Biology and Medicine, UCLA School of Dentistry, PO Box 951668, Los Angeles, CA 90095-1668, USA, T 1 310 825-0203 F 1 310 794-7109 E fqi@dent.ucla.edu

Quantitative analyses of Streptococcus mutans biofilms with quartz crystal microbalance, microjet impingement and confocal microscopy

  • J. Kreth (a1), E. Hagerman (a2), K. Tam (a3), J. Merritt (a4), D. T. W. Wong (a1), B. M. Wu (a2) (a5), N. V. Myung (a3), W. Shi (a1) (a4) and F. Qi (a1)...

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed