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Hatchery-scale trials using cryopreserved spermatozoa of black-lip pearl oyster, Pinctada margaritifera

Published online by Cambridge University Press:  28 June 2011

Belinda Hui ,
Vincent Vonau ,
Jacques Moriceau ,
Roger Tetumu ,
Vincent Vanaa ,
Marina Demoy-Schneider ,
Marc Suquet  and
Gilles Le Moullac
Belinda Hui
Affiliation:
Ifremer, Laboratoire Domestication de l’Huître perlière, Centre du Pacifique, BP 7004, 98719 Taravao, Tahiti, Polynésie Française
Vincent Vonau
Affiliation:
Ifremer, Laboratoire Domestication de l’Huître perlière, Centre du Pacifique, BP 7004, 98719 Taravao, Tahiti, Polynésie Française
Jacques Moriceau
Affiliation:
Ifremer, Laboratoire Domestication de l’Huître perlière, Centre du Pacifique, BP 7004, 98719 Taravao, Tahiti, Polynésie Française
Roger Tetumu
Affiliation:
Ifremer, Laboratoire Domestication de l’Huître perlière, Centre du Pacifique, BP 7004, 98719 Taravao, Tahiti, Polynésie Française
Vincent Vanaa
Affiliation:
Ifremer, Laboratoire Domestication de l’Huître perlière, Centre du Pacifique, BP 7004, 98719 Taravao, Tahiti, Polynésie Française
Marina Demoy-Schneider
Affiliation:
Laboratoire Biodiversité terrestre et marine, UMR CNRS EA4239, Université de la Polynésie Française, BP 6570, 98702 Faa’a, Tahiti, Polynésie Française
Marc Suquet
Affiliation:
UMR 100 Physiologie et Ecophysiologie des Mollusques marins, Ifremer, Site expérimentale d’Argenton, 11 Presqu’île du Vivier, 29840 Argenton en Landunvez, France
Gilles Le Moullac*
Affiliation:
Ifremer, Laboratoire Domestication de l’Huître perlière, Centre du Pacifique, BP 7004, 98719 Taravao, Tahiti, Polynésie Française
*
a Corresponding author: Gilles.Le.Moullac@ifremer.fr
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Abstract

Cryopreservation is a valuable tool for genetic improvement programs. Several bivalve mollusc species have already been the subject of such programs and the Tahitian black pearl oyster industry is now planning the development of selective breeding for desirable traits in Pinctada margaritifera. The ability to cryopreserve spermatozoa would, therefore, offer significant benefits to the cultured black pearl industry. Spermatozoa were cryopreserved with cryoprotectant agent (CPA) 0.7 M trehalose in 0.8 M Me2SO and a two-step freezing process was used: straws were first maintained in nitrogen vapour for 10 minutes, then directly plunged into liquid nitrogen and stored for one week before use. The viability of thawed sperm was 23% lower than that of fresh sperm. When using thawed sperm, therefore, a higher sperm/egg ratio of 100 000:1 was required to reach 80% oocyte fertilization, compared with 100:1 for fresh sperm. Nevertheless, this first demonstration of cryopreserved sperm fertility in black pearl oyster confirms the hatchery applicability of the cryopreservation technique defined here. Monitoring for larval viability during the first 23 days of life revealed no significant differences between the progeny produced with cryopreserved sperm and that produced using fresh sperm.

Keywords

CryopreservationSpermatozoaFertilityLarval rearingPearl oysterPinctada margaritifera
Type
Note
Information
Aquatic Living Resources , Volume 24 , Issue 2 , April 2011 , pp. 219 - 223
Copyright
© EDP Sciences, IFREMER, IRD 2011

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