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Hatchery-scale trials using cryopreserved spermatozoa of black-lip pearl oyster, Pinctada margaritifera

  • Belinda Hui (a1), Vincent Vonau (a1), Jacques Moriceau (a1), Roger Tetumu (a1), Vincent Vanaa (a1), Marina Demoy-Schneider (a2), Marc Suquet (a3) and Gilles Le Moullac (a1)...

Abstract

Cryopreservation is a valuable tool for genetic improvement programs. Several bivalve mollusc species have already been the subject of such programs and the Tahitian black pearl oyster industry is now planning the development of selective breeding for desirable traits in Pinctada margaritifera. The ability to cryopreserve spermatozoa would, therefore, offer significant benefits to the cultured black pearl industry. Spermatozoa were cryopreserved with cryoprotectant agent (CPA) 0.7 M trehalose in 0.8 M Me2SO and a two-step freezing process was used: straws were first maintained in nitrogen vapour for 10 minutes, then directly plunged into liquid nitrogen and stored for one week before use. The viability of thawed sperm was 23% lower than that of fresh sperm. When using thawed sperm, therefore, a higher sperm/egg ratio of 100 000:1 was required to reach 80% oocyte fertilization, compared with 100:1 for fresh sperm. Nevertheless, this first demonstration of cryopreserved sperm fertility in black pearl oyster confirms the hatchery applicability of the cryopreservation technique defined here. Monitoring for larval viability during the first 23 days of life revealed no significant differences between the progeny produced with cryopreserved sperm and that produced using fresh sperm.

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Corresponding author

a Corresponding author: Gilles.Le.Moullac@ifremer.fr

References

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[1]Acosta-Salmón, H., Jerry, D.J., Southgate, P.C., 2007, Effects of cryoprotectant agents and freezing protocol on motility of black-lip pearl oyster (Pinctada margaritifera L.) spermatozoa. Cryobiology 54, 1318.
[2]Adams, S.L., Hessian, P.A., Mladenov, P.V., 2003, Flow cytometric evaluation of mitochondrial function and membrane integrity of marine invertebrate sperm. Invertebr. Reprod. Dev. 44, 4551.
[3]Adams, S.L., Hessian, P.A., Mladenov, P.V., 2004a, Cryopreservation of sea urchin (Evechinus chloroticus) sperm. Cryo Lett. 25, 287300.
[4]Adams, S.L., Smith, J.F., Roberts, R.D., Janke, A.R., Kaspar, H.F., Tervit, H.R., Pugh, P.A., Webb, S.C., King, N.G., 2004b, Cryopreservation of sperm of the Pacific Oyster (Crassostrea gigas): development of a practical method for commercial spat production. Aquaculture 242, 271282.
[5]Adams, S.L., Smith, J.F., Roberts, R.D., Janke, A.R., King, N.G., Tervit, H.R., Webb, S.C., 2008, Application of sperm cryopreservation in selective breeding of the Pacific oyster, Crassostrea gigas (Thunberg). Aquac. Res. 39, 14341442.
[6]Arnaud-Haond, S., Goyard, E., Vonau, V., Herbaut, C., Prou, J., Saulnier, D., 2007, Pearl Formation : Persistence of the Graft During the Entire Process of biomineralization. Mar. Biotechnol. 9, 113116.
[7]Bougrier, S., Rabenomanana, L.D., 1986, Cryopreservation of spermatozoa of the Japanese Oyster, Crassostrea gigas. Aquaculture 58, 277280.
[8] Cabrita, E., Robles, V., Cuñado, S., Wallace, J.C., Sarasquete, C., Herráez,, M.P., 2005, Evaluation of gilthead sea bream, Sparus aurata, sperm quality after cryopreservation in 5ml macrotubes. Cryobiology 50, 273284.
[9]Fauvel, C., Suquet, M., Dreanno, C., Zonno, V., Menu, B., 1998, Cryopreservation of sea bass (Dicentrarchus labrax) spermatozoa in experimental and production stimulating conditions. Aquat. Living Resour. 11, 387394.
[10]Flajshans, M., Cosson, J., Rodina, M., Linhart, O., 2004, The application of image cytometry to viability assessment in dual fluorescence-stained fish spermatozoa. Cell Biol. Inter. 28, 955959.
[11]Graham, J.K., Kunze, E., Hammerstedt, R.H., 1990, Analysis of sperm cell viability, acrosomal integrity, and mitochondrial function using flow cytometry. Biol. Reprod. 43, 5564.
[12] Grzyb, K., Rychlowski, M., Biegniewska, A., Skorkowski, E.F., 2003, Quantitative determination of creatine kinase release from herring (Clupea harengus) spermatozoa induced by tributyltin. Comp. Biochem. Physiol. C 134, 207213.
[13]Gwo, J.C., Wu, C.Y., Chang, W.S.P., Cheng, H.Y., 2003, Evaluation of damage in Pacific oyster (Crassostrea gigas) spermatozoa before and after cryopreservation using comet assay. Cryo. Lett. 24, 171180.
[14] Heasman, M.P., O’Connor, W.A., Frazer, A.W., 1996, Effects of fertilization and incubation factors on the quality and yield of scallop, Pecten fumatus Reeve larvae. Aquac. Res. 27, 505513.
[15]Horvat, A., Wayman, W.R., Dean, J.C., Urbanyi, B., Tiersch, T.R., Mims, S.D., Johnson, D., Jenkins, J.A., 2008, Viability and fertilizing capacity of cryopreserved sperm from three North American acipenseriform species: a retrospective study. J. Appl. Ichthyol. 24, 443449.
[16] Hui B., Demoy-Schneider M., Vonau V., Le Moullac G., Moriceau J., Le Pennec M., Cochard J.C., 2009, Gamete cryopreservation, an asset for a durable pearl farming in French Polynesia. 11th Pacific Science Inter-congress, Tahiti, 2–6 March 2009.
[17]Lyons, L., Jerry, D.R., Southgate, P.C., 2005, Cryopreservation of black-lip pearl oyster (Pinctada margaritifera L.) spermatozoa: effects of cryoprotectants on spermatozoa motility. J. Shellfish Res. 24, 11871190.
[18]Magnesen, T., Bergh, O., Christophersen, G., 2006, Yields of great scallop, Pecten maximus, larvae in a commercial flow-through rearing system in Norway. Aquac. Int. 14, 377394.
[19]Narita, T., Kawamoto, T., Isowa, K., Aoki, H., Hayashi, M., Komaru, A., Ohta, H., 2007, Effects of cryopreservation methods on post-thaw motility of spermatozoa from the Japanese pearl oyster, Pinctada fucata martensii. Cryobiology 54, 1926.
[20]Narita, T., Kawamoto, T., Isowa, K., Aoki, H., Hayashi, M., Komaru, A., Ohta, H., 2008, Fertility of cryopreserved spermatozoa of the Japanese pearl oyster, Pinctada fucata martensii. Aquaculture 275, 178181.
[21]Paniagua-Chávez, C.G., Jenkins, J., Segovia, M., Tiersch, T.R., 2006, Assessment of gamete quality for the eastern oyster (Crassostrea virginica) by use of fluorescent dyes. Cryobiology 53, 128138.
[22]Pouvreau, S., Gangnery, A., Tiapari, J., Lagarde, F., Garnier, M., Bodoy, A., 2000, Gametogenic cycle and reproductive effort of the tropical blacklip pearl oyster, Pinctada margaritifera (Bivalvia: Pteriidae), cultivated in Takapoto atoll (French Polynesia). Aquat. Living Resour. 13, 3748.
[23]Rico-Villa, B., Woerther, P., Mingant, C., Lepiver, D., Pouvreau, S., Hamon, M., Robert, R., 2008, A flow-through rearing system for ecophysiological studies of Pacific oyster Crassostrea gigas larvae. Aquaculture 282, 5460.
[24]Santos, A.E., Nascimento, I.A., 1989, Influence of gamete density, salinity and temperature on the normal embryonic development of the mangrove oyster Crassostrea rhizophorae. Aquaculture 73, 295307.
[25]Sarkis, S., Helm, M., Hohn, C., 2006, Larval rearing of calico scallops, Argopecten gibbus, in a flow-through system. Aquac. Int. 14, 527538.
[26]Smith, J.F., Pugh, P.A., Tervit, H.R., Roberts, R.D., Janke, A.R., Kaspar, H.F., Adams, S.L., 2001, Cryopreservation of shellfish sperm, eggs and embryos. Proc. N. Z. Soc. Anim. Prod. 61, 3134.
[27]Song, Y.P., Suquet, M., Quéau, I., Lebrun, L., 2009, Setting of a procedure for experimental fertilization of Pacific oyster (Crassostrea gigas) oocytes. Aquaculture 287, 311314.
[28]Wada, S.K., 1963, Studies on the fertilization of Pelecypod gamete. Increase in maturity and accomplishment of fertilization of pearl oyster gametes in ammoniacal sea water. Mem. Fac. Fish. Kagoshima Univ. 12, 92108.
[29]Yankson, K., Moyse, J., 1991, Cryopreservation of the spermatozoa of Crassostrea tulipa and three other oysters. Aquaculture 97, 259267.

Keywords

Hatchery-scale trials using cryopreserved spermatozoa of black-lip pearl oyster, Pinctada margaritifera

  • Belinda Hui (a1), Vincent Vonau (a1), Jacques Moriceau (a1), Roger Tetumu (a1), Vincent Vanaa (a1), Marina Demoy-Schneider (a2), Marc Suquet (a3) and Gilles Le Moullac (a1)...

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