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Mapping ESTs to the TSC1 candidate interval by use of the ‘Science 96’ transcript map

Published online by Cambridge University Press:  01 September 1997

J. WOLFE ,
S. JEREMIAH ,
J. YOUNG ,
M. W. BURLEY ,
H. STEWART ,
M. McCULLEY ,
C. GRANT ,
K. NAZ  and
S. POVEY
J. WOLFE
Affiliation:
The Galton Laboratory, Department of Biology, University College London, 4, Stephenson Way, London NW1 2HE
S. JEREMIAH
Affiliation:
The MRC Human Biochemical Genetics Unit, The Galton Laboratory, Department of Biology, University College London, 4, Stephenson Way, London NW1 2HE
J. YOUNG
Affiliation:
The MRC Human Biochemical Genetics Unit, The Galton Laboratory, Department of Biology, University College London, 4, Stephenson Way, London NW1 2HE
M. W. BURLEY
Affiliation:
The MRC Human Biochemical Genetics Unit, The Galton Laboratory, Department of Biology, University College London, 4, Stephenson Way, London NW1 2HE
H. STEWART
Affiliation:
The Galton Laboratory, Department of Biology, University College London, 4, Stephenson Way, London NW1 2HE
M. McCULLEY
Affiliation:
The Galton Laboratory, Department of Biology, University College London, 4, Stephenson Way, London NW1 2HE
C. GRANT
Affiliation:
The Galton Laboratory, Department of Biology, University College London, 4, Stephenson Way, London NW1 2HE
K. NAZ
Affiliation:
The Galton Laboratory, Department of Biology, University College London, 4, Stephenson Way, London NW1 2HE
S. POVEY
Affiliation:
The MRC Human Biochemical Genetics Unit, The Galton Laboratory, Department of Biology, University College London, 4, Stephenson Way, London NW1 2HE
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Abstract

The transcription map of the human genome published by Schuler et al. (1996) is a valuable resource in which approximately one quarter of all human genes have been mapped with respect to genetic framework markers using radiation hybrids. We have taken information from this map to provide potential genes within the TSC1 candidate region on chromosome 9q34. In so doing we have been able to provide an independent assay of the quality of the radiation hybrid mapping by using somatic cell hybrids and a 2 Mb cosmid contig covering the TSC1 region as mapping tools. In addition, we have built sequence contigs of ESTs for 25 clusters. This has shown that about 20% of the relevant EST clusters in the Unigene resource (Boguski & Schuler 1995) contain chimaeric clones.

Type
Research Article
Information
Annals of Human Genetics , Volume 61 , Issue 5 , September 1997 , pp. 401 - 409
Copyright
© University College London 1997

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