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Patterns and controls of nitrogen stable isotopes of particulate organic matter in subtropical lakes

Published online by Cambridge University Press:  19 March 2010

Binhe Gu  and
Claire L. Schelske
Binhe Gu*
Affiliation:
Fishery College, Guangdong Ocean University, 40 East Jiefang Road, Zhanjiang, Guangdong, 52488, China Southwest Research and Education Center, University of Florida, 2685 SR 29 N, Immokalee, FL 34142, USA
Claire L. Schelske
Affiliation:
Department of Geological Sciences and Land Use and Environmental Change Institute, University of Florida, Gainesville, FL 32611, USA
*
gubinhe@gmail.com
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Abstract

Nitrogen stable isotope composition (δ15N) of particulate organic matter (POM) has been used to infer dominant nitrogen cycling processes in lakes. However, very few studies have compared the isotope variations in lakes along trophic state and other biogeochemical gradients. Here we report an analysis of δ15NPOM and selected environmental variables from 96 subtropical lakes to assess the patterns and controls of isotope variations. Results indicate that δ15NPOM values varied from −2.8 to 13.2‰ and were not significantly correlated with total phosphorus (TP), total nitrogen (TN) and chlorophyll a (Chl a). The 15N depletion in POM was found across the entire trophic gradient and likely reflected contributions from planktonic nitrogen fixation. The 15N enrichment was attributed to high primary production and the contributions of anthropogenic wastes in eutrophic lakes, and the presence of microzooplankton in the water samples of the oligotrophic lakes. The δ15NPOM was negatively correlated with water color and positively correlated with pH. Because water color is indicative of light availability which affects phytoplankton growth and pH is significantly correlated with Chl a concentration in the study lakes, the close relationship between water color, pH and δ15NPOM therefore suggests primary productivity-driven isotope fractionation. Results from this study did not reveal the importance of trophic state, nitrogen concentration and surface area to the variations of δ15NPOM and points to the complex interactions of nitrogen cycling processes in lakes with diverse biogeochemical features.

Keywords

Nitrogen fixationnitrogen stable isotopesparticulate organic mattersubtropical lakestrophic statewater color
Type
Research Article
Information
Annales de Limnologie - International Journal of Limnology , Volume 46 , Issue 1 , 2010 , pp. 1 - 7
Copyright
© EDP Sciences, 2010

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References

APHA, 1998. Standard methods for the examination of water and wastewater, 20th edition, Washington, DC.
Bade, D.L., Carpenter, S.R., Cole, J.J., Hanson, P.C. and Hesslein, R.H., 2004. Controls of δ13C-DIC in lakes: Geochemistry, lake metabolism, and morphometry. Limnol. Oceanogr. , 49, 1160–1172.
Bootsma, H.A., Hecky, R.E., Hesslein, R.H. and Turner, G.F., 1996. Food partitioning among Lake Malawi nearshore fishes as revealed by stable isotope analyses. Ecology , 77, 1286–1290. CrossRef
Carpenter S.R., Caraco N.F., Correll D.L., Howarth R.W., Sharpley A.N. and Smith V.H., 1998. Nonpoint pollution of surface waters with phosphorus and nitrogen. Ecol. Appl., 8, 559–568.
Chapman A.D. and Schelske C.L., 1997. Recent appearance of Cylindrospermopsis (Cyanobacteria) in five hypereutrophic Florida lakes. J. Phycol., 33, 191–195.
Delwiche C.C. and Steyn P.L., 1970. Nitrogen isotope fractionation in soils and microbial reactions. Environ. Sci. Tech., 4, 929–935.
Estep M.L.F. and Vigg S., 1985. Stable carbon and nitrogen isotope tracers of trophic dynamics in natural populations and fisheries of the Lahontan Lake system, Nevada. Can. J. Fish. Aquat. Sci., 42, 1712–1719.
Gondwe, M.J., Guildford, S.J. and Hecky, R.E., 2008. Planktonic nitrogen fixation in Lake Malawi/Nyasa. Hydrobiologia , 596, 251–267. CrossRef
Gu, B., 2009. Variations and controls of nitrogen stable isotopes in particulate organic matter of lakes. Oecologia , 160, 421–431. CrossRef
Gu, B. and Alexander, V., 1993. Estimation of N2 fixation based on differences in the natural abundance of 15N among freshwater N2-fixing and non-N2-fixing algae. Oecologia , 96, 43–48. CrossRef
Gu B., Schell D.M. and Alexander V., 1994. Stable carbon and nitrogen isotopic analysis of the plankton food web in a subarctic lake. Can. J. Fish. Aquat. Sci., 51, 1338–1344.
Gu B., Schelske C.L. and Brenner M., 1996. Relationship between sediment and plankton isotope ratios (δ13C and δ15N) and primary productivity in Florida lakes. Can. J. Fish. Aquat. Sci., 53, 875–883.
Gu B., Chapman A.D. and Schelske C.L., 2006. Factors controlling seasonal variations in stable isotope composition of particulate organic matter in a soft water eutrophic lake. Limnol. Oceanogr., 51, 2837–2848.
Gu B., Schelske C.L. and Waters M., submitted. Patterns and controls of carbon stable isotope composition of particulate organic matter in subtropical lakes. Fundam. Appl. Limol.
Hadas O., Altabet M.A. and Agnihotri R., 2009. Seasonally varying nitrogen isotope biogeochemistry of particulate organic matter in Lake Kinneret, Israel. Limnol. Oceanogr., 54, 75–85.
Havens K.E., Phlips E.J., Cichra M.F. and Li B., 1998. Light availability as a possible regulator of cyanobacteria species composition in a shallow subtropical lake. Freshw. Biol., 39, 547–556.
Havens, K.E., Gu, B., Fry, B. and Kendall, C., 2003. Stable isotope food web analysis of a large subtropical lake: Alternative explanations for 15N enrichment of pelagic vs. littoral fisheries. The Scientific World Journal , 3, 613–622. CrossRef
Hoering T.C. and Ford H.T., 1960. The isotope effect in the fixation of nitrogen by Azotobacter. J. Amer. Chem. Soc., 82, 376–378.
Hoyer M.V., Gu B. and Schelske C.L., 1998. Sources of organic carbon in the food webs of two Florida lakes indicated by stable isotopes. In: Jeppesen E.M., Sondergaard M., Sondergaard M. and Christofferson K. (eds.), The structure role of submerged macrophytes in lakes, Springer, Berlin-Heidelberg-New York, 326–330.
Jäntti H., 2007. The spatial and temporal variation of nitrogen fixation in aquatic environments, Master Thesis, University of Jyväskylä, 46 p.
Lehmann M.F., Bernasconi S.M., McKenzie J.A., Barbieri A., Simona M. and Veronesi M., 2004. Seasonal variation of the δ13C and δ15N of particulate and dissolved carbon and nitrogen in Lake Lugano: constraints on biogeochemical cycling in a eutrophic lake. Limnol. Oceanogr., 49, 415–429.
MacGregor B.J., Van Mooy B., Baker B.J., Mellon M., Moisander P.H., Paerl H.W., Zehr J., Hollander D. and Stahl D., 2001. Microbiological, molecular biological and stable isotopic evidence for nitrogen fixation in the open waters of Lake Michigan. Environ. Microbiol., 3, 205–219.
Montoya J.P. and McCarthy J.J., 1995. Isotopic fractionation during nitrate uptake by phytoplankton grown in continuous culture. J. Plankton Res., 17, 439–464.
Mooy B.V., MacGregor B., Hollander D., Nealson K. and Stahl D., 2001. Evidence for tight coupling between active bacteria and particulate organic carbon during seasonal stratification of Lake Michigan. Limnol. Oceanogr., 46, 1202–1208.
Owen J.S., Mitchell M.J. and Michener R.H., 1999. Stable nitrogen and carbon isotonic composition of seston and sediment in two Adirondack lakes. Can. J. Fish. Aquat. Sci., 56, 2186–2192.
Owens N.J.P., 1987. Natural variations in 15N in the marine environment. Adv. Mar. Biol., 24, 389–451.
Patoine A., Graham M.D. and Leavitt P.R., 2006. Spatial variation of nitrogen fixation in lakes of the northern Great Plains. Limnol. Oceanogr., 51, 1665–1677.
Peterson B.J. and Fry B., 1987. Stable isotopes in ecosystem studies. Ann. Rev. Ecol. Syst., 18, 293–320.
Post, D.M., 2002. Using stable isotopes to estimate trophic position: Models, methods, and assumptions. Ecology , 83, 703–718. CrossRef
Savage, C., 2005. Tracing the influence of sewage nitrogen in a coastal ecosystem using stable nitrogen isotopes. Ambio , 34, 143–148. CrossRef
Syväranta J., Tiirola M. and Jones R.I., 2008. Seasonality in lake pelagic δ15N values: patterns, possible explanations, and implications for food web baseline. Fundam. Appl. Limnol., 172, 255–262.
Teranes J.L. and Bernasconi S.M., 2000. The record of nitrate utilization and productivity limitation provided by δ15N values in lake organic matter – a study of sediment trap and core sediments from Baldeggersee, Switzerland. Limnol. Oceanogr., 45, 801–813.
Valiela, I., Geist, M., Mcclelland, J. and Tomasky, G., 2000. Nitrogen loading from watersheds to estuaries: verification of the Waquoit Bay nitrogen loading model. Biogeochemistry , 49, 277–293. CrossRef
Vander Zanden M.J., Vadeboncoeur Y., Diebel M.W. and Jeppensen E., 2005. Primary consumer stable nitrogen isotopes as indicators of nutrient source. Environ. Sci. Technol., 39, 7509–7515.
Waser N.A.D., Harrison P.J., Nielsen B., Calvert S.E. and Turpin D.H., 1998. Nitrogen isotope fractionation during the uptake and assimilation of nitrate, nitrite, ammonium, and urea by a marine diatom. Limnol. Oceanogr., 43, 215–224.
Xie, Y.X., Xiong, Z.Q., Xing, G.X., Sun, G.Q. and Zhu, Z.L., 2007. Assessment of nitrogen pollutant sources in surface waters of Taihu Lake region. Pedosphere , 17, 200–208. CrossRef
Zeng, Q.F., Kong, F.X., Zhang, E.L., Tan, X. and Wu, X.D., 2008. Seasonality of stable carbon and nitrogen isotopes within the pelagic food web of Taihu Lake. Ann. Limnol. - Int. J. Lim. , 44, 55–60.