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The effect of routine experimental procedures on physiological parameters in mice kept under different husbandry conditions

Published online by Cambridge University Press:  11 January 2023

MK Meijer ,
K Kramer ,
R Remie ,
BM Spruijt ,
LFM van Zutphen  and
V Baumans
MK Meijer*
Affiliation:
Department of Animals, Science and Society, Divisions of Laboratory Animal Science & Ethology and Welfare, Utrecht University, PO Box 80166, 3508 TD, Utrecht, The Netherlands
K Kramer
Affiliation:
Department of Safety and Environmental Affairs, Free University, Amsterdam, The Netherlands
R Remie
Affiliation:
Solvay Pharmaceuticals BV, Weesp, The Netherlands
BM Spruijt
Affiliation:
Department of Animals, Science and Society, Divisions of Laboratory Animal Science & Ethology and Welfare, Utrecht University, PO Box 80166, 3508 TD, Utrecht, The Netherlands
LFM van Zutphen
Affiliation:
Department of Animals, Science and Society, Divisions of Laboratory Animal Science & Ethology and Welfare, Utrecht University, PO Box 80166, 3508 TD, Utrecht, The Netherlands
V Baumans
Affiliation:
Department of Animals, Science and Society, Divisions of Laboratory Animal Science & Ethology and Welfare, Utrecht University, PO Box 80166, 3508 TD, Utrecht, The Netherlands Karolinska Institute, Stockholm, Sweden
*
* Correspondence and request for reprints: m.k.meijer@vet.uu.nl
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Abstract

Laboratory animals are frequently subjected to routine procedures, such as injections or the withdrawal of blood samples. Acute stress caused by such procedures is associated with physiological changes that can have a strong impact on experimental results. This study investigated the integrated effects of cage enrichment, social housing and handling on the acute stress response of animals subjected to routine experimental procedures. Female mice of two inbred strains (BALB/c and C57BL/6) were housed under either minimal husbandry conditions (MH: no cage enrichment, infrequent handling and a period of individual housing) or enriched husbandry conditions (EH: with cage enrichment, frequent handling and social housing at all times). One mouse in each cage was implanted with a radio-telemetry transmitter for measuring heart rate (HR) and body temperature (BT). The animals were subjected to intraperitoneal injections or short periods of restraint. In addition to telemetry measurements, thymus weight and tyrosine hydroxylase (TH) activity were assessed. It was found that individual housing under MH conditions, as compared with social housing under EH conditions, elevated both basal HR and BT, and significantly elevated the relative recovery time following routine experimental procedures. Thymus weight and TH activity suggested a long-term stress response under MH conditions following individual housing, although the influence of transmitter implantation and (repeated) acute stress remains to be investigated. The results emphasise that husbandry conditions should be taken into account when evaluating physiological measures after routine procedures.

Keywords

animal welfarehusbandry conditionsmiceradio-telemetryroutine proceduresstress response
Type
Research Article
Information
Animal Welfare , Volume 15 , Issue 1 , February 2006 , pp. 31 - 38
Copyright
© 2006 Universities Federation for Animal Welfare

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References

Borsini, F, Lecci, A, Volterra, G and Meli, A 1989 A model to measure anticipatory anxiety in mice? Psychopharmacology (Berlin) 98: 207–211CrossRefGoogle Scholar
Clement, JG, Mills, PA and Brockway, BP 1989 Use of telemetry to record body temperature and activity in mice. Journal of Pharmacological Methods 21: 129–140CrossRefGoogle ScholarPubMed
Einstein, R, Rowan, C, Billing, R and Lavidis, N 2000 The use of telemetry to refine experimental technique. In: Balls, M, van, Zeller A-M and Halder, ME (eds) Progress in the Reduction, Refinement and Replacement of Animal Experimentation pp 1187–1197. Elsevier Science BV: Amsterdam, The NetherlandsGoogle Scholar
Gärtner, K, Büttner, D, Döhler, K, Friedel, R, Lindena, J and Trautschold, I 1980 Stress response of rats to handling and experimental procedures. Laboratory Animals 14: 267–274CrossRefGoogle ScholarPubMed
Harkin, A, Connor, TJ, O'Donnell, JM and Kelly, JP 2002 Physiological and behavioral responses to stress: what does a rat find stressful? Lab Animal (New York) 31: 42–50Google ScholarPubMed
Izumi, J, Washizuka, M, Hayashi-Kuwabara, Y, Yoshinaga, K, Tanaka, Y, Ikeda, Y, Kiuchi, Y and Oguchi, K 1997 Evidence for a depressive-like state induced by repeated saline injections in Fischer 344 rats. Pharmacology Biochemistry and Behavior 57: 883–888Google ScholarPubMed
Kramer, K, van Acker, SABE, Voss, HP, Grimbergen, JA, van der Vijgh, WJF and Bast, A 1993 Use of telemetry to record electrocardiogram and heart rate in freely moving mice. Journal of Pharmacological and Toxicological Methods 30: 209–215CrossRefGoogle ScholarPubMed
Manser, CE 1992 The Assessment of Stress in Laboratory Animals. The Royal Society for the Prevention of Cruelty to Animals (RSPCA): Horsham, UKGoogle Scholar
Mouse Genome Database 2001a Inbred strains of mice: BALB. Mouse Genome Informatics Web Site. The Jackson Laboratory, Bar Harbor, Maine, USA. http://www.informatics.jax.org (accessed 19 October 2005)Google Scholar
Mouse Genome Database 2001b Inbred strains of mice: C57BL. Mouse Genome Informatics Web Site. The Jackson Laboratory, Bar Harbor, Maine, USA. http://www.informatics.jax.org (accessed 19 October 2005)Google Scholar
Pekow, CA and Baumans, V 2003 Common nonsurgical techniques and procedures. In: Hau J and van Hoosier GL (eds) Handbook of Laboratory Animal Science p 367. CRC Press: Boca Raton, Florida, USAGoogle Scholar
Poole, T 1997 Happy animals make good science. Laboratory Animals 31: 116–124CrossRefGoogle ScholarPubMed
Sharp, J, Azar, T and Lawson, D 2003 Does cage size affect heart rate and blood pressure of male rats at rest or after procedures that induce stress-like responses? Contemporary Topics in Laboratory Animal Science 42: 8–12Google ScholarPubMed
Sharp, JL, Zammit, TG, Azar, TA and Lawson, DM 2002 Stress-like responses to common procedures in male rats housed alone or with other rats. Contemporary Topics in Laboratory Animal Science 41: 8–14Google ScholarPubMed
Späni, D, Arras, M, König, B and Rülicke, T 2003 Higher heart rate of laboratory mice housed individually vs in pairs. Laboratory Animals 37: 54–62CrossRefGoogle ScholarPubMed
Tuli, JS, Smith, JA and Morton, DB 1995a Corticosterone, adrenal and spleen weight in mice after tail bleeding, and its effect on nearby animals. Laboratory Animals 29: 90–95CrossRefGoogle ScholarPubMed
Tuli, JS, Smith, JA and Morton, DB 1995b Stress measurements in mice after transportation. Laboratory Animals 29: 132–138CrossRefGoogle ScholarPubMed
van den Berg, CL, van Ree, JM and Spruijt, BM 2000 Morphine attenuates the effects of juvenile isolation in rats. Neuropharmacology 39: 969–976CrossRefGoogle ScholarPubMed
van der Heyden, JAM, Zethof, TJJ and Olivier, B 1997 Stress-induced hyperthermia in singly housed mice. Physiology & Behavior 62: 463–470CrossRefGoogle ScholarPubMed
Witte, PU and Matthaei, H 1980 Microchemische Methoden für Neurobiologische Untersuchungen. Springer: Berlin, Germany [Title translation: Microchemical Methods for Neurobiological Research]Google Scholar
Yang, Y and Gordon, CJ 1996 Ambient temperature limits and stability of temperature regulation in telemetered male and female rats. Journal of Thermal Biology 21: 353–363CrossRefGoogle Scholar
Zethof, TJJ, van der Heyden, JAM, Tolboom, JTBM and Olivier, B 1994 Stress-induced hyperthermia in mice: a methodological study. Physiology & Behavior 55: 109–115CrossRefGoogle ScholarPubMed