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Tissue-specific differences in insulin binding affinity and insulin receptor concentrations in skeletal muscles, adipose tissue depots and liver of cattle and sheep

Published online by Cambridge University Press:  18 August 2016

P. D. McGrattan ,
A. R. G. Wylie  and
J. Nelson
P. D. McGrattan
Affiliation:
Northern Ireland Regional Genetics Centre, Belfast City Hospital Trust, Belfast BT9 7AB, UK
A. R. G. Wylie
Affiliation:
Department of Agriculture for Northern Ireland (DANI) and School of Agriculture and Food Science, The Queen’s University of Belfast, Newforge Lane, Belfast BT9 5PX, UK
J. Nelson
Affiliation:
School of Biology and Biochemistry, The Queen’s University of Belfast Medical Biology Centre, Lisburn Road, Belfast, BT9 7BL, UK
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Abstract

Differences in insulin binding affinity and in concentrations of insulin receptor, were found in a variety of tissues taken, at slaughter, from mature steers (701 (s.d. 23) kg) and growing lambs (47 (s.d. 2·1) kg). In both species, liver had lower insulin binding affinity than skeletal muscles m. pectineus m. longissimus dorsi and m. rectus capitis (all P < 0·001) and subcutaneous, omental and perirenal adipose depots (all P < 0·001). Site-specific differences in affinity for insulin existed between adipose depots (subcutaneous < omental, P < 0·05; subcutaneous < perirenal, P < 0·001) and between tissue-types (subcutaneous fat < m. pectineus skeletal muscle, P < 0·05; m. rectus capitis < perirenal fat, P < 0·05) in steers. In lambs also, receptor affinity for insulin differed between tissue-type (m. longissimus dorsi < perirenal fat, P < 0·05; m. rectus capitis < subcutaneous fat, P < 0·05 and m. rectus capitis < perirenal fat, P < 0·001) but lambs did not show the adipose depot-specific differences in insulin affinity observed in steers. Insulin receptor concentration differed between adipose depots (subcutaneous < omental, P < 0·05; subcutaneous < perirenal, P < 0·01) and between tissue-type (m. pectineus < perirenal fat P < 0·05) in steers and perirenal and subcutaneous adipose depots of lambs had higher receptor concentrations than m. longissimus dorsi and m. pectineusP < 0·001). This is the first study to demonstrate, in any species, differences in insulin receptor binding affinity and receptor concentration in a wide range of tissues (liver, skeletal muscles and adipose depots) from the same individual. Such differences in meat-producing animals could, through effects on tissue sensitivity and/or responsiveness to insulin, influence nutrient partitioning to tissues and affect overall rates of lipid storage and net protein synthesis.

Keywords

binding affinitycattleinsulinreceptorssheep
Type
Research Article
Information
Animal Science , Volume 71 , Issue 3 , December 2000 , pp. 501 - 508
Copyright
Copyright © British Society of Animal Science 2000

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