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Rumen metabolism and nitrogen flow to the small intestine in steers offered Lolium perenne containing different levels of water-soluble carbohydrate

  • M. R. F. Lee (a1), L. J. Harris (a1), J. M. Moorby (a1), M. O. Humphreys (a1), M. K. Theodorou (a1), J. C. MacRae (a2) and N. D. Scollan (a1)...

Abstract

Eight Hereford ✕ Friesian steers were used to investigate the effect of feeding Lolium perenne (L) forage containing elevated levels of water-soluble carbohydrate (WSC) on rumen metabolism and nitrogen (N) absorption from the small intestine. The steers were offered ad libitum access to one of two varieties with matched heading dates (Ba11353, high WSC, HS; AberElan, intermediate WSC, control) cut at different times of the day to accentuate WSC differentials, zero-grazed for 21 days. This was followed by a 14-day period where the animals were on grass silage to provide a covariate intake. Although the total N concentration was similar for the two grasses, all other measured values were significantly different. The dry matter (DM) concentration of HS was greater than that of the control (202 v. 167 g DM per kg; P 0·01). WSC and in-vitro dry matter digestibility (IVDMD) were 243 and 161 g/kg DM, and 0·61 and 0·56 for HS and control, respectively. In contrast, acid- and neutral-detergent fibre were 251 and 296 g/kg DM and 480 and 563 g/kg DM for HS compared with control, respectively. DM intake was increased (9·3 v. 6·7 kg/day; P 0·001) for HS animals and this contributed significantly towards higher flows of non-ammonia N to the duodenum as well as increased absorption of amino acids from the small intestine. This DM intake response was partly due to the elevation in DM concentration of HS. However fresh weight intake was increased proportionately by ca. 0·15 (P 0·05) in animals on HS compared with control. Rumen ammonia levels were lower (14·0 and 26·4 mg N per l; P 0·001) and concentrations of rumen propionate higher (P 0·01) and acetate lower (P 0·01; increasing the glucogenic: lipogenic volatile fatty acid ratio) in animals on HS compared with control. However, the efficiency of microbial protein synthesis (15·9 and 17·8 g microbial nitrogen per kg organic matter apparently digested) and flow of N to the duodenum per unit N intake (0·84 and 0·93) for HS and control, respectively, were similar across both diets.

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Agricultural Research Council. 1980. The nutrient requirements of ruminant livestock. Commonwealth Agricultural Bureaux, Unwin Brothers, Old Woking.
Armstrong, D. G. and Hutton, J. B. 1972. Fate of nitrogenous compounds entering the small intestine. In Digestion and metabolism in the ruminant (ed. Mc, I. W.Donald and A. Warner, C. I.), pp. 432447. University of New England Publishing Unit, Armidale, Australia.
Beever, D. E. and Siddons, R. C. 1986. Digestion and metabolism in the grazing ruminant. In Control of digestion and metabolism in ruminants (ed. Milligan, L. P. Grovum, W. L. and Dobson, A.), pp. 479497. Simon and Schuster, Englewood, NJ.
Beever, D. E., Terry, R. A., Cammell, S. B. and Wallace, A. S. 1978. The digestion of spring and autumn harvested perennial ryegrass in sheep. Journal of Agricultural Science, Cambridge 90: 463470.
Buttery, P. J. 1977. Aspects of the biochemistry of rumen function and their implication in ruminant productivity. In Recent advances in animal nutrition (ed. Haresign, W. and Lewis, D.), pp. 824,Nottingham University Press.
Carruthers, V. R. and Neil, P. G. 1997. Milk production and ruminal metabolites from cows offered two pasture diets supplemented with non-structural carbohydrate. New Zealand Journal of Agricultural Research 40: 513521.
Chiy, P. C. and Philips, C. J. C. 1999. The rate of intake of sweet, salty and bitter concentrates by dairy cows. Animal Science 68: 731740.
Corbett, J. L. 1987. Energy and protein utilisation by grazing animals. In Temperate pastures: their production, use and management (ed. Wheeler, J. L. Pearson, C. J. and Robards, G. E.), pp. 341355. CSIRO Publication.
Cozzi, G., Bittante, G. and Polan, C. E. 1993. Comparison of fibrous materials as modifiers of in-situ ruminal degradation of corn gluten meal. Journal of Dairy Science 76: 11061113.
Faichney, G. J. 1975. The use of markers to partition digestion within the gastro-intestinal tract of ruminants. In Digestion and metabolism in the ruminant (ed. Mc, I. W.Donald and A. Warner, C. I.), pp. 277291. University of New England Publishing Unit, Armidale, NSW.
Henning, P. H., Steyn, Dxs. G. and Meissner, H. H. 1993. Effect of synchronisation of energy and nitrogen supply on ruminal characteristics and microbial growth. Journal of Animal Science 71: 25162528.
Jarrett, I. G. 1948. The production of rumen and abomasal fistulae in sheep. Journal of the Council of Scientific and Industrial Research 21: 311315.
Jones, D. I. H. and Hayward, M. V. 1975. The effect of pepsin treatment of herbage on the prediction of dry matter digestibility from solubility in fungal cellulase solutions. Journal of the Science of Food and Agriculture 26: 711718.
Lawes Agricultural Trust. 1995. Genstat 5. Rothamsted Experimental Station, Harpenden, Hertfordshire, UK.
Lee, M. R. F. 2001. The use of novel forages to increase the synchrony of nitrogen and energy release in the reticulo-rumen. Ph.D. thesis, University of Aberdeen.
Lee, M. R. F., Jones, E. L., Moorby, J. M., Humphreys, M. O., Theodorou, M. K., MacRae, J. C. and Scollan, N. D. 2001. Production responses from lambs grazed on Lolium perenne selected for elevated water-soluble carbohydrate concentration. Animal Research 50: 441449.
Lobley, G. E., Connell, A., Lomax, M. A., Brown, D. S., Milne, E., Calder, A. G. and Farningham, D. A. H. 1995. Hepatic detoxification of ammonia in the liver; possible consequences for amino acid metabolism. British Journal of Nutrition 73: 667685.
McGrath, D. 1988. Seasonal variation in the water-soluble carbohydrates of perennial and italian ryegrass under cutting conditions. Irish Journal of Agricultural Research 27: 131139.
MacRae, J. C., Bruce, L. A. and Brown, D. S. 1995. Efficiency of utilisation of absorbed amino acids in growing lambs given forage and forage: barley diets. Journal of Animal Science 61: 277284.
MacRae, J. C. and Lobley, G. E. 1982. Some factors influencing thermal energy losses during metabolism of ruminants. Livestock Production Science 9: 447456.
MacRae, J. C. and Lobley, G. E. 1986. Interactions between energy and protein. In Control of digesta metabolism in ruminants (ed. Milligan, L. P. Grovum, W. L. and Dobson, A.), pp. 367385. Prentice Hall, Englewood Cliffs, NJ.
MacRae, J. C., Smith, J. S., Dewey, P. J. S., Brewer, A. C., Brown, D. S. and Walker, A. 1985. The efficiency of utilisation of metabolisable energy and apparent absorption of amino acids in sheep given spring and autumn harvested dried grass. British Journal of Nutrition 54: 197209.
MacRae, J. C. and Ulyatt, M. J. 1974. Quantitative digestion of fresh forage by sheep. 2. The sites of digestion of some nitrogenous constituents. Journal of Agricultural Science, Cambridge 82: 309319.
Milano, G. D., Hotson-Moore, A. and Lobley, G. E. 2000. Influence of hepatic ammonia removal on ureagenesis, amino acid utilization and energy metabolism in the ovine liver. British Journal of Nutrition 83: 307315.
Moorby, J. M. and Theobald, V. J. 1999. The effect of duodenal ammonia infusions on milk production and nitrogen balance of the dairy cow. Journal of Dairy Science 82: 24402442.
Moorby, J. M., Miller, L. A., Evans, R. T., Scollan, N. D., Theodorou, M. K. and MacRae, J. C. 2001. Milk production and N partitioning in early lactation dairy cows offered perennial ryegrass containing a high concentration of water soluble carbohydrates. Proceedings of the British Society of Animal Science, 2001, p. 6.
Newbold, J. R. and Rust, S. R. 1992. Effect of asynchronous N and energy on growth of ruminal bacteria in batch culture. Journal of Animal Science 70: 538546.
Nocek, J. E. and Russell, J. B. 1988. Protein and energy as an integrated system. Relationship of ruminal protein and carbohydrate availability to microbial synthesis and milk production. Journal of Dairy Science 71: 20702107.
Obara, Y. and Dellow, D. W. 1994. Influence of energy supplementation on nitrogen kinetics in the rumen and urea metabolism. Japanese Agricultural Research Quarterly 28: 143-149.
Obara, Y., Dellow, D. W. and Nolan, J. V. 1991. The influence of energy rich supplements on nitrogen kinetics in ruminants. In Physiological aspects of digestion and metabolism in ruminants (ed. Tsuda, T., Sasaki, Y. and Kawashima, R.), pp. 515534. Academic Press, San Diego, CA.
Pollock, C. J. and Jones, T. 1979. Seasonal patterns of fructan metabolism in forage grasses. The New Phytologist 83: 915.
Poppi, D. 1990. Manipulation of nutrient supply to animals at pasture, opportunities and consequences. Fifth Australasian Association for Animal Production, Taipei, pp. 4279.
Robinson, P. H., Veira, D. M. and Ivan, M. 1998. Influence of supplemental protein quality on rumen fermentation, rumen microbial yield, forestomach digestion, and intestinal amino acid flow in late lactation Holstein cows. Canadian Journal of Animal Science 78: 95105.
Rooke, J. A., Lee, N. H. and Armstrong, D. G. 1987. The effects of intraruminal infusions of urea, casein, glucose syrup, and a mixture of casein and glucose syrup on nitrogen digestion in the rumen of cattle receiving grasssilage diets. British Journal of Nutrition 57: 8994.
Satter, L. D. and Slyter, L. L. 1974. Effect of ammonia concentration on ruminal microbial protein production in vitro. British Journal of Nutrition 32: 199208.
Siever-Kelly, C., Leury, B. J., Gatford, K. L., Simpson, R. J. and Dove, H. 1999. Spray-topping annual grass pasture with glyphosate to delay loss of feeding value during summer. II. Herbage intake, digestibility, and diet selection in penned sheep. Australian Journal of Agricultural Research 50: 465474.
Symonds, H. W., Mather, D. L. and Collis, K. A. 1981. The maximum capacity of the liver of the adult dairy cow to metabolise ammonia. British Journal of Nutrition 46: 481486.
Thomas, T. A. 1977. An automated procedure for the determination of soluble carbohydrates in herbage. Journal of the Science of Food and Agriculture 28: 639642.
Thornton, R. F. and Minson, D. J. 1972. The relationship between voluntary intake and mean apparent retention time in the rumen. Australian Journal of Agricultural Research 23: 871877.
Ulyatt, M. J., Beever, D. E., Thomson, D. J., Evans, R. T. and Haines, M. J. 1980. Measurement of nutrient supply at pasture. Proceedings of the Nutrition Society 39: 67A.
Van Soest, P. J., Robertson, J. B. and Lewis, B. A. 1991. Methods for dietary fiber, neutral detergent fiber and non starch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74: 35833597.
Van Soest, P. J. and Wine, R. H. 1967. Use of detergents in the analysis of fibrous feeds. IV. Determination of plant and cell wall constituents. Journal of the Association of Official Analytical Chemists 50: 5055.
Williams, C. H., David, D. J. and Iismaa, O. 1962. The determination of chromic oxide in faeces samples by atomic absorption spectrophotometry. Journal of Agricultural Science, Cambridge 59: 381385.
Zhu, W. Y., Theodorou, M. K., Longlands, A. C., Nielson, B. B., Dijkstra, J. and Trinci, A. P. J. 1996. Growth and survival of anaerobic fungi in batch and continuous culture. Anaerobe 2: 2937.

Keywords

Rumen metabolism and nitrogen flow to the small intestine in steers offered Lolium perenne containing different levels of water-soluble carbohydrate

  • M. R. F. Lee (a1), L. J. Harris (a1), J. M. Moorby (a1), M. O. Humphreys (a1), M. K. Theodorou (a1), J. C. MacRae (a2) and N. D. Scollan (a1)...

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