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Plasma biochemical values in the guanaco (Lama guanicoe) and a comparison with the sheep

Published online by Cambridge University Press:  02 September 2010

M. D. Fraser  and
J. M. Moorby
M. D. Fraser
Affiliation:
Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth SY23 3EB
J. M. Moorby
Affiliation:
Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth SY23 3EB
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Abstract

An initial experiment was conducted to investigate the variability of selected metabolites in the plasma from guanacos. A second experiment directly compared plasma biochemical values for guanacos with those for sheep. During the first experiment, jugular blood samples were collected from five mature castrated guanacos using an embedded experimental design. Weekly blood samples were collected at the same time (09.00 h) on the same day each week for 7 weeks. Daily blood samples were collected at the same time each day (09.00 h) during week 5. On day 2 of week 5, blood samples were collected every 3 h from 09.00 h for a 24-h period. No evidence of a cyclical pattern of plasma parameters was observed on a weekly, daily or 3-h basis. During the second experiment, the metabolic profiles of 11 mature castrated guanacos and 11 mature barren ewes (Merino × Welsh Mountain) were compared. Significant differences in plasma concentrations of all metabolites except urea-nitrogen (guanacos -15·42 mmolll, sheep - 15·60 (s.e.d. 1·506) mmolll) were found with values for guanacos v. sheep as follows: glucose (7·63 v. 3·63 (s.e.d. 0·268) mmolll); acetate (0·26 v. 0·48 (s.e.d. 0·035) mmol/l); β hydroxybutyrate (0·06 v. 0·50 (s.e.d. 0·019) mmol/l); albumin (33·4 v. 29·5 (s.e.d. 0·93) g/l); and total protein (53·8 v. 65·6 (s.e.d. 2·12) g/l); (P < 0·001 for all previous variables); non-esterified fatty acids (0·48 v. 0·29 (s.e.d. 0·048) meq per 1; P < 0·01) and a-amino N (2·44 v. 2·66 (s.e.d. 0·088) mmolll; P < 0·05). This study indicates that the reference plasma metabolite concentrations of sheep are not suitable alternatives for use for nutritional or veterinary purposes with guanacos, but those of llamas or alpacas are. The results also suggest that energy capture and transport in camelids may be markedly different from that in conventional ruminants.

Keywords

camelidaeglucose3-hydroxybutyric acidlama guanicoemetabolites
Type
Research Article
Information
Animal Science , Volume 66 , Issue 1 , February 1998 , pp. 209 - 216
Copyright
Copyright © British Society of Animal Science 1998

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References

Bradstreet, R. B. 1969. The Kjeldhal method for organic nitrogen. Academic Press Inc., New York.Google Scholar
Britton, R. and Krehbiel, C. 1993. Nutrient metabolism by gut tissues Journal of Dairy Science 76: 2125–2131.CrossRefGoogle ScholarPubMed
Clarke, P. M. and Payton, M. A. 1983. An enzymatic assay for acetate in spent bacterial culture supernatants Analytical Biochemistry 130: 402–405.CrossRefGoogle ScholarPubMed
Fowler, M. E. 1989. Medicine and surgery of South American camelids. Iowa State University Press.Google Scholar
Fowler, M. E. and Zinkl, J. G. 1989. Reference values for hematologic and serum biochemical values in llamas (Lama glama). American Journal of Veterinary Research 50: 2049–2053.Google ScholarPubMed
Gustafsson, A. H. and Palmquist, D. L. 1993. Diurnal variation of rumen ammonia, serum urea, and milk urea in dairy cows at high and low yields Journal of Dairy Science 76: 475–484.CrossRefGoogle ScholarPubMed
Hastings, B. E. and Gasgoyne, S. C. 1992. Trace mineral levels in the guanaco (Lama guanicoe). Veterinary Record 131: 14–15.CrossRefGoogle ScholarPubMed
Hinderer, S. and Engelhardt, W. von. 1975. Urea metabolism in the Ilama. Comparative Biochemistry Physiology 52A: 619–622.CrossRefGoogle Scholar
Kaneko, J. J. 1989. Clinical biochemistry of domestic fourth edition, pp. 886–891. Academic Press, New York.Google Scholar
Kunz, P. L., Blum, J. W., Hart, I. C., Bickel, H. and Landis, J. 1985. Effects of different energy intakes before and after calving on food intake, performance and blood hormones and metabolites in dairy cows Animal Production 40: 219–231.Google Scholar
Lassen, E. D., Pearson, E. G., Long, P., Schmotzer, W. B., Kaneps, A. J. and Riebold, T. W. 1986. Clinical biochemical values of llamas: reference values American Journal of Veterinary Research 47: 2278–2280.Google ScholarPubMed
Lewis, D. 1957. Blood-urea concentration in relation to protein utilization in the ruminant Journal of Agricultural Science, Cambridge 48: 438–446.CrossRefGoogle Scholar
MacRae, J. C. and Armstrong, D. G. 1968. Enzyme method for determination of a-linked glucose polymers in biological materials Journal of the Science of Food and Agriculture 19: 578–581.CrossRefGoogle Scholar
Oddy, V. H. 1974. A semi-automated method for determination of plasma alpha-amino nitrogen Clinica Chimica Acta 51: 151–156.CrossRefGoogle Scholar
Payne, J. M., Dew, S. M., Manston, R. and Faulks, M. 1970. The use of a metabolic profile test in dairy herds Veterinary Record 87: 150–158.CrossRefGoogle ScholarPubMed
Payne, J. M., Rowlands, G. J., Manston, R., Dew, S. M. and Parker, W. H. 1974. A statistical appraisal of the results of the metabolic profile tests on 191 herds in the B.V.A./ A.D.A.S. joint exercise in animal health and productivity. British Veterinary Journal 130: 34–43.CrossRefGoogle ScholarPubMed
Reynolds, C. K. and Huntington, G. H. 1988. Partition of portal-drained visceral net flux in beef steers. 1. Blood flow and net flux of oxygen, glucose, and nitrogenous compounds across stomach and post-stomach tissues. British Journal of Nutrition 60: 539–551.CrossRefGoogle ScholarPubMed
Reynolds, C. K., Tyrrell, H. F. and Armentano, L. E. 1992. Effects of mesenteric vein n-butyrate infusion on liver metabolism by beef steers Journal of Animal Science 70: 2250–2261.CrossRefGoogle ScholarPubMed
Russel, A. J. F. 1993. The role of fibre producing animals in European agriculture Fine Fibre News 2: 1–7.Google Scholar
Russel, A. J. F., Doney, J. M. and Reid, R. L. 1967. The use of biochemical parameters in controlling nutritional state in pregnant ewes, and the effect of undernourishment during pregnancy on lamb birth-weight Journal of Agricultural Science, Cambridge 68: 351–358.CrossRefGoogle Scholar
Russel, A. J. F. and Wright, I. A. 1983. The use of blood metabolites in the determination of energy status in beef cows Animal Production 37: 335–343.Google Scholar
Simons, J. A., Waldron, D. L. and Hennessy, D. P. 1993. Clinical biochemical reference ranges for female alpacas (Lama pacos). Comparative Biochemistry and Physiology 105B: 603–608.Google Scholar
Stangassinger, M. and Giesecke, D. 1986. Splanchnic metabolism of glucose and related energy substrates. In Control of digestion and metabolism in ruminants (ed. Milligan, L., Grovum, W. L. and Dobson, A.), pp. 347–366. Prentice-Hall, Englewood Cliffs, NJ 07632.Google Scholar
Sykes, A. R. 1976. An assessment of the value of plasma urea nitrogen and albumin concentrations as monitors of the protein status of sheep. In The use of blood metabolites in animal production (ed. Lister, D.), British Society of Animal Production occasional publication no. 1, pp. 143–154.Google Scholar
Thomas, T. A. 1977. A n automated procedure for the determination of soluble carbohydrate s in herbage Journal of the Science of Food and Agriculture 28: 639–642.CrossRefGoogle Scholar
Van Soest, P. J. 1963. Use of detergent s in the analysis of fibrous feeds. II. A rapid method for the determination of fibre and lignin. Journal of the Association of Official Analytical Chemists 46: 829–835.Google Scholar
Van Soest, P. J. and Wine, R. H. 1967. Use of detergents in the analysis of fibrous feeds. IV. Determination of plant and cell-wall constituents. Journal of the Association of Official Analytical Chemists 50: 50–55.Google Scholar