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Effects of level of nitrogen fertilizer application and various nitrogenous supplements on milk production and nitrogen utilization of dairy cows given grass silage-based diets

Published online by Cambridge University Press:  18 August 2016

K.J. Shingfield ,
S. Jaakkola  and
P. Huhtanen
S. Jaakkola
Affiliation:
Animal Production Research, Agricultural Research Centre of Finland, FIN 31600 Jokioinen, Finland
P. Huhtanen
Affiliation:
Animal Production Research, Agricultural Research Centre of Finland, FIN 31600 Jokioinen, Finland
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Abstract

Sixteen Finnish Ayrshire dairy cows were used in a cyclic change-over experiment with four 28-day experimental periods and a 4 ✕ 2 factorial arrangement of treatments to evaluate the effects of dietary nitrogen (N) source on dry-matter (DM) intake, diet digestibility, milk production, blood metabolite concentrations and N utilization. Experimental treatments consisted of two silages prepared from timothy and meadow fescue swards fertilized with either 52 or 104 kg N per ha (SL and SH, respectively) offered ad libitum supplemented with one of four concentrate supplements offered at 10 kg/day. A basal concentrate (control) was formulated from (g/kg on an air-dry basis) barley (307), oats (460), molassed sugar-beet pulp (200) and vitamins and minerals (33). Three isonitrogenous concentrates were prepared by replacement of basal concentrate ingredients (g/kg) with urea (14·4), wheat-gluten meal (WGM; 57·2) and heat-moisture treated mechanically expelled rapeseed cake (RSC; 188), respectively. Harvesting of secondary growths receiving higher applications of fertilizer N increased silage N content (19·2 and 23·6 g/kg DM, for SL and SH, respectively) but decreased water-soluble carbohydrate concentrations (respective values 149 and 93 g/kg DM). Increases in N fertilizer had no effect on silage DM intake, output of energy-corrected milk, milk fat or milk protein secretion, while provision of additional N in concentrate supplements improved all of these parameters. However, the magnitude of silage DM intake responses to additional N in concentrates was higher (P < 0·05) for SL than SH silage-based diets. Increases in dietary N content derived from silage or inclusion of urea, WGM or RSC in concentrate supplements led to a decline in the proportion of N intake secreted in milk. Relative to N derived from silage or urea, WGM and RSC supplements had beneficial effects on milk production. Both the quantity and quality of dietary N sources should be considered in future attempts to improve the efficiency of on-farm N utilization.

Keywords

dairy cowsdigestibilitygrass silagemilk productionnitrogen fertilizer.
Type
Ruminant nutrition, behaviour and production
Information
Animal Science , Volume 73 , Issue 3 , December 2001 , pp. 541 - 554
Copyright
Copyright © British Society of Animal Science 2001

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References

Agricultural and Food Research Council. 1992. Technical Committee on Responses to Nutrients, report no. 9. Nutritive requirements of ruminant animals: protein. Nutrition, Abstracts and Reviews, Series B 62: 787–835.Google Scholar
Ahvenjärvi, S., Vanhatalo, A., Huhtanen, P. and Varvikko, T. 1999. Effects of supplementation of a grass silage and barley diet with urea, rapeseed meal and heat-moisture treated rapeseed cake on milk production and omasal digesta flow in lactating dairy cows. Acta Agriculturæ Scandinavica, Section A Animal Science 49: 179–189.Google Scholar
Association of Official Analytical Chemists. 1990. Official methods of analysis, 15th edition. Association of Official Analytical Chemists, Arlington, VA.Google Scholar
Broderick, G. A. and Clayton, M. K. 1997. A statistical evaluation of animal and nutritional factors influencing concentrations of milk urea nitrogen. Journal of Dairy Science 80: 2964–2971.Google Scholar
Castillo, A. R., Kebreab, E., Beever, D. E. and France, J. 2000. A review of efficiency of nitrogen utilisation in lactating dairy cows and its relationship with environmental pollution. Journal of Animal and Feed Sciences 9: 1–32.CrossRefGoogle Scholar
Chamberlain, D. G., Choung, J. J. and Robertson, S. 1992. Protein nutrition of dairy cows receiving grass silage diets: effects of feeding a protein supplement of unbalanced amino acid composition. Journal of the Science of Food and Agriculture 60: 425–430.CrossRefGoogle Scholar
Chamberlain, D. G., Martin, P. A. and Robertson, S. 1989. Optimizing compound feed use in dairy cows with high intakes of silage. In Recent advances in animal nutrition (ed. Haresign, W. and Cole, D.J.A.), pp. 175–193. Butterworths, London.CrossRefGoogle Scholar
Davis, A. W. and Hall, W. B. 1969. Cyclic change-over designs. Biometrica 56: 283–293.Google Scholar
Dewhurst, R. J., Aston, K., Fisher, W. J., Evans, R. T., Dhanoa, M. S. and McAllan, A. B. 1999. Comparison of energy and protein sources offered at low levels in grass-silage-based diets for dairy cows. Animal Science 68: 789–799.CrossRefGoogle Scholar
Dewhurst, R. J., Mitton, A. M., Offer, N. W. and Thomas, C. 1996. Effects of the composition of grass silages on milk production and nitrogen utilization by dairy cows. Animal Science 62: 25–34.CrossRefGoogle Scholar
Friedel, K. 1990. [The estimation of the energetic feeding value of roughages by means of a cellulase method.] Wissenschaftliche Zeitung Universitet Rostock, N-Reihe 39: 78–86.Google Scholar
Gustafsson, A. H. and Palmquist, D. L. 1993. Diurnal variation of rumen ammonia, serum urea, and milk urea in dairy cows at high and low yields. Journal of Dairy Science 76: 475–484.CrossRefGoogle ScholarPubMed
Haacker, K., Block, H. J. and Weissbach, F. 1983. [On the colorimetric determination of lactic acid in silages with p-hydroxydiphenyl.] Archiv für Tierernährung 33: 505–512.Google Scholar
Hermansen, J. E., Ostersen, S. and Aaes, O. 1994. Effects of the levels of N fertilizer, grass and supplementary feeds on nitrogen composition and renneting properties of milk from cows at pasture. Journal of Dairy Research 61: 179–189.Google Scholar
Huhtanen, P. 1998. Supply of nutrients and productive responses in dairy cows given diets based on restrictively fermented grass silage. Agricultural and Food Science in Finland 7: 219–250.Google Scholar
Huhtanen, P., Blauwiekel, R. and Saastamoinen, I. 1998. Effects of intraruminal infusions of propionate and butyrate with two different protein supplements on milk production and blood metabolites in dairy cows receiving grass silage-based diet. Journal of the Science of Food and Agriculture 77: 213–222.Google Scholar
Huhtanen, P. and Heikkilä, T. 1996. Effects of physical treatment of barley and rapeseed meal in dairy cows given grass silage-based diets. Agricultural and Food Science in Finland 5: 399–412.CrossRefGoogle Scholar
Huida, L. 1982. Gas chromatographic determination of water and ethanol in silage by internal standard method. Journal of the Scientific Society of Agricultural Science in Finland 54: 137–143.Google Scholar
Huida, L., Väätäinen, H. and Lampila, M. 1986. Comparison of dry matter contents in grass silage as determined by oven drying and gas chromatographic water analyses. Annales Agriculturae Fenniae 25: 215–230.Google Scholar
Institut National de la Recherche Agronomique. 1989. Ruminant nutrition: recommended allowances and feed tables (ed. Jarrige, R.). INRA publications, John Libbey Eurotext, London.Google Scholar
Jarvis, S. C., Scholefield, D. and Pain, B. F. 1995. Nitrogen cycling in grazing systems. In Nitrogen fertilization in the environment (ed. Bacon, P. E.), pp. 381–419. Marcel Dekker, New York.Google Scholar
Jarvis, S. C., Wilkins, R. J. and Pain, B. F. 1996. Opportunities for reducing the environmental impact of dairy farming managements: a systems approach. Grass and Forage Science 51: 21–31.CrossRefGoogle Scholar
Keady, T. W. J., Mayne, C. S. and Fitzpatrick, D. A. 2000. Prediction of silage feeding value from the analysis of the herbage at ensiling and effects of nitrogen fertilizer, date of harvest and additive treatment on grass silage composition. Journal of Agricultural Science, Cambridge 134: 353–368.Google Scholar
Keady, T. W. J., Murphy, J. J. and Fitzgerald, S. 1995. The effects of level of nitrogen fertilizer application to silage swards on subsequent silage composition, intake and animal performance of lactating dairy cattle. Animal Science 60: 515 (abstr.).Google Scholar
Keady, T. W. J. and O´Kiely, P. 1996. An evaluation of the effects of rate of nitrogen fertilization of grassland on silage fermentation, in-silo losses, effluent production and aerobic stability. Grass and Forage Science 51: 350–352.CrossRefGoogle Scholar
Khalili, H., Huhtanen, P., Jaakkola, S. and Varvikko, T. 1995. The effects of ruminal and duodenal casein infusion on dry matter (DM) intake of red clover silage and rumen pool size, digestion and passage kinetics of neutral detergent fibre (NDF). Annales de Zootechnique 44: (suppl.) 243.Google Scholar
Khorasani, G. R., Boer, G. de, Robinson, P. H. and Kennelly, J. J. 1992. Effect of canola fat on ruminal and total tract digestion, plasma hormones, and metabolites in lactating dairy cows. Journal of Dairy Science 75: 492–501.Google Scholar
Kristensen, V. F., Munksgaard, L., Møller, P. D., Andersen, P. E., Kristiansen, K. R., Munksgaard, L., Werner, H. and Skovberg, E. B. 1987. [The effect of nitrogen fertilizer on the protein value of grass silage for dairy cows and on the quality of milk.] Beretning, Fællesudsvalget for Statens Planteavls-og Hysdyrbrugsforsøg, no. 11.Google Scholar
Licitra, G., Hernandez, T. M. and Van Soest, P. J. 1996. Standardization of procedures for nitrogen fractionation of ruminant feed. Animal Feed Science and Technology 57: 347–358.CrossRefGoogle Scholar
Littell, R. C., Milliken, G. A., Stroup, W. W. and Wolfinger, R. D. 1996. SAS® system for mixed models. SAS Institute Inc., Cary, NC.Google Scholar
McCullough, H. 1967. The determination of ammonia in whole blood by direct colorimetric method. Clinica Chimica Acta 17: 297–304.CrossRefGoogle ScholarPubMed
McDonald, P., Henderson, A. R. and Heron, S. J. E. 1991. The biochemistry of silage, second edition. Chalcombe Publications, Marlow.Google Scholar
Metcalf, J. A., Wray-Cahen, D., Chettle, E. E., Sutton, J. D., Beever, D. E., Crompton, L. A., MacRae, J. C., Bequette, B. J. and Backwell, F. R. C. 1996. The effect of dietary crude protein as protected soybean meal on mammary metabolism in the lactating dairy cow. Journal of Dairy Science 79: 603–611.Google Scholar
Ministry of Agriculture, Fisheries and Food. 1975. Energy allowances and feeding systems for ruminants. Technical bulletin no 33. Ministry of Agriculture, Fisheries and Food. London.Google Scholar
Ministry of Agriculture, Fisheries and Food. 1984. Energy allowances and feeding systems for ruminants. ADAS reference book 433. Her Majesty’s Stationery Office, London.Google Scholar
Mo, M. 1979. The potential of conserved forage for milk production. In Forage conservation in the 80´s (ed. Thomas, C.), British Grassland Society, occasional symposium no. 11, pp. 154–163.Google Scholar
National Reseach Council. 2001. Nutrient requirements of dairy cattle, seventh revised edition. National Academy Press, Washington, DC.Google Scholar
Oldham, J. D. 1984. Protein energy relationships in dairy cows. Journal of Dairy Science 67: 1090–1114.Google Scholar
Peyraud, J. L. and Astigarraga, L. 1998. Review of the effect of nitrogen fertilization on the chemical composition, intake, digestion and nutritive value of fresh herbage: consequences on animal nutrition and N balance. Animal Feed Science and Technology 72: 235–259.Google Scholar
Peyraud, J. L., Astigarraga, L. and Faverdin, P. 1997. Digestion of fresh perennial ryegrass fertilized at two levels of nitrogen by lactating dairy cows. Animal Feed Science and Technology 64: 155–171.Google Scholar
Rinne, M., Jaakkola, S., Varvikko, T. and Huhtanen, P. 1999. Effects of the type and amount of rapeseed feed on milk production. Acta Agriculturæ Scandinavica, Section A Animal Science 49: 137–148.Google Scholar
Robertson, J. B. and Van Soest, P. J. 1981. The detergent system of analysis and its application to human foods. In The analyses of dietary fibre in foods (ed. James, W.D.T. and Theander, O.), pp. 123–158. Marcell Dekker, New York.Google Scholar
Shingfield, K. J. and Huhtanen, P. 1998. Evaluation of nutritional factors influencing milk urea concentrations. Journal of Dairy Science 81: (suppl. 1) 316 (abstr.).Google Scholar
Shingfield, K. J., Jaakkola, S. and Huhtanen, P. 1999a. Comparison of the effects of dietary nitrogen supplements on the intake and milk production of Finnish Ayrshire dairy cows fed grass silage-based diets. Proceedings of the 12th international silage conference, SLU, Uppsala, Sweden (ed. Pauly, T.), pp. 159–160.Google Scholar
Shingfield, K. J., Jokela, M., Kaustell, K., Huhtanen, P. and Nousiainen, J. 1999b. Association between protein feeding and reproductive efficiency in the dairy cow: specific emphasis on protein feeding in Finland. Agricultural and Food Science in Finland 8: 365–392.Google Scholar
Sjaunja, L. O., Baevre, L., Junkkarinen, L., Pedersen, J. and Setälä, J. 1990. A Nordic proposal for an energy corrected milk (ECM) formula. 27th session of the International Committee of Recording and Productivity of Milk Animals, Paris, pp. 156–157.Google Scholar
Somogyi, M. 1945. A new reagent for the determination of sugars. Journal of Biological Chemistry 160: 61–68.CrossRefGoogle Scholar
Statistical Analysis Systems Institute. 1989. SAS/STAT® user´s guide, version 6, fourth edition. SAS Institute Inc., Cary, NC.Google Scholar
Thomas, C. and Rae, R. C. 1988. Concentrate supplementation of silage for dairy cows. In Nutrition and lactation in the dairy cow (ed. Garnsworthy, P. C.), pp. 327–354. Butterworths, London.Google Scholar
Tuori, M. 1992. Rapeseed meal as a supplementary protein for dairy cows on grass silage-based diet, with the emphasis on the Nordic AAT-PBV feed protein evaluation system. Agricultural Science in Finland 1: 367–439.Google Scholar
Tuori, M., Kaustell, K. and Huhtanen, P. 1998. Comparison of the protein evaluation systems of feeds for dairy cows. Livestock Production Science 55: 33–46.Google Scholar
Tuori, M., Kaustell, K., Valaja, J., Aimonen, E., Saarisalo, E. and Huhtanen, P. 1996. [Feed tables and feeding recommendations, second edition.] Yliopistopaino, Helsinki.Google Scholar
Vanhatalo, A., Huhtanen, P., Toivonen, V. and Varvikko, T. 1999. Response of dairy cows fed grass silage diets to abomasal infusions of histidine alone or in combinations with methionine and lysine. Journal of Dairy Science 82: 2674–2685.Google Scholar
Van Keulen, J. and Young, B. A. 1977. Acid insoluble ash as a natural marker for digestibility studies. Journal of Animal Science 44: 282–287.Google Scholar
Van Soest, P. J., Robertson, J. B. and Lewis, B. A. 1991. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74: 3583–3597.Google Scholar
Webster, A. J. F. 1992. The metabolisable protein system for ruminants. In Recent advances in animal nutrition (ed. Garnsworthy, P.C., Haresign, W. and J.A.|Cole, D.), pp. 93–110. Butterworths, London.Google Scholar
Whitelaw, F. G., Milne, J. S., Ørskov, E.R. and Smith, J. S. 1986. The nitrogen and energy metabolism of lactating cows given abomasal infusion of casein. British Journal of Nutrition 55: 537–556.Google Scholar